Abstract
Objective
Stimulated Raman histology (SRH) offers efficient and accurate intraoperative neuropathological tissue analysis without procedural alteration to the diagnostic specimen. However, there are limited data demonstrating one-to-one tissue comparisons between SRH and traditional frozen sectioning. This study explores the non-inferiority of SRH as compared to frozen section on the same piece of tissue in neurosurgical patients.
Methods
Tissue was collected over a 1-month period from 18 patients who underwent resection of central nervous system lesions. SRH and frozen section analyses were compared for diagnostic capabilities as well as assessed for quality and condition of tissue via a survey completed by pathologists.
Results
SRH was sufficient for diagnosis in 78% of specimens as compared to 94% of specimens by frozen section of the same specimen. A Fisher’s exact test determined there was no significant difference in diagnostic capability between the two groups. Additionally, both quality of SRH and condition of tissue after SRH were deemed to be non-inferior to frozen section.
Conclusions
This study provides further evidence for the non-inferiority of SRH techniques. It is also the first study to demonstrate SRH accuracy using one-to-one tissue analysis in neuropathological specimens.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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All authors contributed to the preparation of this manuscript. EHE, AR and FA: literature search was conducted. EHE, AR, FA, RSA and MH: primary writing of the body of the manuscript was performed. MH, SW, GH, PDC, JAE, JAB, DJL, RSA and FA: data collection was conducted. EHE and MH: figures were created. All authors were involved in the editing and revision of this manuscript. All authors read and approved the final manuscript.
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Einstein, E.H., Ablyazova, F., Rosenberg, A. et al. Stimulated Raman histology facilitates accurate diagnosis in neurosurgical patients: a one-to-one noninferiority study. J Neurooncol 159, 369–375 (2022). https://doi.org/10.1007/s11060-022-04071-y
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DOI: https://doi.org/10.1007/s11060-022-04071-y