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Neonatal Hypothyroidism Affects the Adenine Nucleotides Metabolism in Astrocyte Cultures from Rat Brain

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Abstract

Neonatal hypothyroidism is associated with multiple and severe brain alterations. We recently demonstrated a significant increase in hydrolysis of AMP to adenosine in brain of hypothyroid rats at different ages. However, the origin of this effect was unclear. Considering the effects of adenine nucleotides to brain functions and the harmful effects of neonatal hypothyroidism to normal development of the central nervous system, in this study we investigated the metabolism of adenine nucleotides in hippocampal, cortical and cerebellar astrocyte cultures from rats submitted to neonatal hypothyroidism. ATP and AMP hydrolysis were enhanced by 52 and 210%, respectively, in cerebellar astrocytes from hypothyroid rats. In hippocampus of hypothyroid rats, the 47% increase in AMP hydrolysis was significantly reverted when the astrocytes were treated with T3. Therefore, the imbalance in the ATP and adenosine levels in astrocytes, during brain development, may contribute to some of the effects described in neonatal hypothyroidism.

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Acknowledgement

This work was supported by Conselho de Desenvolvimento Científico e Tecnológico (CNPq-Brazil), Programas de Núcleos de Excelência (PRONEX-Brazil) and Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil).

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Avenida Ramiro Barcellos 2600-ANEXO, 90035-003, Porto Alegre, RS, Brazil

    Elizandra Braganhol, Alessandra Nejar Bruno, Luci Bavaresco, João José Freitas Sarkis & Ana Maria Oliveira Battastini

  2. Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900, São Paulo, SP, Brazil

    Maria Luiza M. Barreto-chaves

  3. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Avenida Ramiro Barcellos 2600-ANEXO, 90035-003, Porto Alegre, RS, Brazil

    Ana Maria Oliveira Battastini

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  1. Elizandra Braganhol
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  2. Alessandra Nejar Bruno
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  3. Luci Bavaresco
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  4. Maria Luiza M. Barreto-chaves
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  5. João José Freitas Sarkis
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  6. Ana Maria Oliveira Battastini
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Corresponding author

Correspondence to Ana Maria Oliveira Battastini.

Additional information

Elizandra Braganhol and Alessandra Nejar Bruno are first authors.

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Braganhol, E., Bruno, A.N., Bavaresco, L. et al. Neonatal Hypothyroidism Affects the Adenine Nucleotides Metabolism in Astrocyte Cultures from Rat Brain. Neurochem Res 31, 449–454 (2006). https://doi.org/10.1007/s11064-006-9041-y

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  • DOI: https://doi.org/10.1007/s11064-006-9041-y

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