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In vivo Effects of the Anatoxin-a on Striatal Dopamine Release

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Abstract

Anatoxin-a is an important neurotoxin that acts a potent nicotinic acetylcholine receptor agonist. This characteristic makes anatoxin-a an important tool for the study of nicotinic receptors. Anatoxin-a has been used extensively in vitro experiments, however anatoxin-a has never been studied by in vivo microdialysis studies. This study test the effect of anatoxin-a on striatal in vivo dopamine release by microdialysis.

The results of this work show that anatoxin-a evoked dopamine release in a concentration-dependent way. Atropine had not any effect on dopamine release evoked by 3.5 mM anatoxin-a. However, perfusion of nicotinic antagonists mecamylamine and α-bungarotoxin induced a total inhibition of the striatal dopamine release. Perfusion of α7*-receptors antagonists, metillycaconitine or α-bungarotoxin, partially inhibits the release of dopamine stimulated by anatoxin-a. These results show that anatoxin-a can be used as an important nicotinic agonist in the study of nicotinic receptor by in vivo microdialysis technique and also support further in vivo evidences that α7*nicotinic AChRs are implicated in the regulation of striatal dopamine release.

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Acknowledgements

This research was supported by grants from the Direction General de Investigación-Ministerio de Ciencia y Tecnología (BQU2002–00083) and the Xunta de Galicia (PGIDT02PXIB30101PR).

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Authors and Affiliations

  1. Department of Functional Biology and Health Sciences, Faculty of Biology, University of Vigo, Vigo, Pontevedra, Spain

    F. Campos, R. Durán, L. Vidal & M. Alfonso

  2. Department of Physiology, Center of Biological Sciences, UFPA, Belém, PA, Brasil

    L. R. F. Faro

  3. Department of Functional Biology and Health Sciences, Faculty of Biology, University of Vigo, Vigo, Pontevedra, Spain

    F. Campos

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Campos, F., Durán, R., Vidal, L. et al. In vivo Effects of the Anatoxin-a on Striatal Dopamine Release. Neurochem Res 31, 491–501 (2006). https://doi.org/10.1007/s11064-006-9042-x

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