Abstract
Normobaric hyperoxia (NBO) is applied for treatment of various clinical conditions related to hypoxia, but it can potentially also induce generation of reactive oxygen species, causing cellular damage. In this study, we examined the effects of 60 h NBO treatment on lipid and protein oxidative damage and activity of superoxide dismutase (Mn-SOD) in brain mitochondria of guinea pigs. Despite significant stimulation of Mn-SOD expression and activity the NBO treatment resulted in accumulation of markers of oxidative lesions, including lipid peroxidation (conjugated dienes, thiobarbituric acid reactive substances) and protein modification (bityrosines, adducts with lipid peroxidation products, oxidized thiols). When inhaled O2 was enriched with oxygen cation, O •+2 , the Mn-SOD expression and activity were stimulated to similar extend, but lipid peroxidation and protein oxidation were prevented. These results suggest that long-term NBO treatment causes oxidative stress, but enrichment of inhaled oxygen by oxygen cation can protect the brain again adverse effects of hyperoxia.
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Acknowledgments
This work was partially supported by grants VEGA 1/0028/11 and VVCE APVV 0064/07 from the Ministry of Education and Science of the Slovak Republic and project “Identification of Novel Markers in Diagnostic Panel of Neurological Diseases”, code:26220220114 co-financed from EC sources and European Regional Development Fund.
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Tatarkova, Z., Engler, I., Calkovska, A. et al. Effect of Long-Term Normobaric Hyperoxia on Oxidative Stress in Mitochondria of the Guinea Pig Brain. Neurochem Res 36, 1475–1481 (2011). https://doi.org/10.1007/s11064-011-0473-7
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DOI: https://doi.org/10.1007/s11064-011-0473-7