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Effect of green light on the amount and activity of NDH-1–PSI supercomplex in Synechocystis sp. strain PCC 6803

  • Published:
Photosynthetica

Abstract

Cyanobacterial NDH-1 interacts with PSI to form NDH-1–PSI supercomplex. CpcG2, a linker protein for the PSI-specific peripheral antenna CpcG2-phycobilisome, is essential for stabilization of the supercomplex. Green light (GL) increased the expression of CpcG2 but had little effect, if any, on the expression of NDH-1 and PSI, when compared to the abundance of these components under red light (RL). The increased expression of CpcG2 intensified the band of NDH-1–PSI supercomplex after blue-native gel electrophoresis of the thylakoid membrane, possibly by stabilizing the supercomplex. The activity of NDH-1-dependent cyclic electron transport around PSI increased when cells grown under RL were transferred to a low intensity GL but was suppressed when cells were grown under high intensities of GL. The functionality of PSI showed the same trend. We thus conclude that GL increases the expression of CpcG2, thereby increasing the abundance of the NDH-1–PSI supercomplex and its activity at low GL but not at higher GL.

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Abbreviations

BN-PAGE:

blue-native-PAGE

CBB:

Coomassie Brilliant Blue

Chl:

chlorophyll

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

GL:

green light

NDH-CET:

NDH-1-dependent cyclic electron transport around PSI

PBS:

phycobilisome

P m :

the maximal P700 change

RL:

red light

Synechocystis 6803:

Synechocystis sp. strain PCC 6803

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Authors and Affiliations

  1. College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai, 200234, China

    F. Gao & W. Ma

  2. Bioscience Center, Nagoya University, Chikusa, Nagoya, 464–8601, Japan

    T. Ogawa

Authors
  1. F. Gao
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  2. T. Ogawa
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  3. W. Ma
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Corresponding author

Correspondence to W. Ma.

Additional information

Acknowledgements: This work was supported by the National Natural Science Foundation of China (grant nos. 31700205, 31370270, 31570235, and 31770259), China Postdoctoral Science Foundation (grant nos. 2015M581643 and 2017T100304), and Shanghai Science and Technology Committee Key Project (grant no. 17070502900).

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Gao, F., Ogawa, T. & Ma, W. Effect of green light on the amount and activity of NDH-1–PSI supercomplex in Synechocystis sp. strain PCC 6803. Photosynthetica 56, 316–321 (2018). https://doi.org/10.1007/s11099-018-0790-z

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  • DOI: https://doi.org/10.1007/s11099-018-0790-z

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