Skip to main content
SpringerLink
Log in

Hairy root and tissue cultures of Leucojum aestivum L.—relationships to galanthamine content

  • Original Paper
  • Published:
Phytochemistry Reviews Aims and scope Submit manuscript

Abstract

Galanthamine, an isoquinoline alkaloid acetylcholinesterase inhibitor, is an important agent used all around the world for the symptomatic treatment of senile dementia of the Alzheimer’s type. The production of this metabolite and the availability of the plant are limited and prompted the search for an alternative way to obtain this valuable metabolite using in vitro cultures of Leucojum aestivum L. It is known that cell differentiation level shows a major influence upon the accumulation of alkaloids. For this reason, tissue cultures of L. aestivum showing different stages of morphogenesis controlled by exogenous growth regulators were established. Agrobacterium rhizogenes strain LBA 9402 has been tested for its capacity to induce hairy roots of this monocotyledonae plant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bonhomme V, Laurain-Mattar D, Lacoux J, Fliniaux MA, Jacquin A (2000a) Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A,B,C genes only. J Biotechnol 81:151–158

    Article  CAS  Google Scholar 

  • Bonhomme V, Laurain-Mattar D, Fliniaux MA (2000b) The effects of rol C gene on hairy root induction, development and tropane alkaloid production by Atropa belladonna. J Nat Prod 63:1249–1252

    Article  CAS  Google Scholar 

  • Diop MF, Ptak A, Chrétien F, Henry M, Chapleur Y, Laurain-Mattar D (2006) Galanthamine content of bulbs and in vitro cultures of Leucojum aestivum L. Nat Prod Commun 1(6):475–479

    CAS  Google Scholar 

  • Eichhorn J, Takada T, Kita Y, Zenk MH (1998) Biosynthesis of the Amaryllidaceae alkaloid galanthamine. Phytochemistry 49(4):1037–1047

    Article  CAS  Google Scholar 

  • Hooykaas PJJ, Shilperoort RA (1992) Agrobacterium and plant genetic engineering. Plant Mol Biol 19:15–38

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi S, Kihara M, Yuasa K, Imakura Y, Shingu T, Kato A, Hashimoto T (1985) Alkaloidal constituents of Leucojum aestivum L. (Amaryllidaceae). Chem Pharm Bull 33(12):5259–5263

    Google Scholar 

  • Küenburg B, Czollner L, Fröhlich J, Jordis U (1999) Development of a pilot scale process for the anti-Alzheimer drug (-)-galanthamine using large scale phenolic oxidative coupling and crystallisation-induced chiral conversion. Org Process Res Dev 3:425–431

    Article  Google Scholar 

  • Le Flem-Bonhomme V, Laurain-Mattar D, Fliniaux MA (2004) Hairy root induction of Papaver somniferum var album, a difficult-to-transform plant, by A rhizogenes LBA 9402. Planta 218:890–893

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Palazón J, Cusido RM, Gonzalio J, Bonfill M, Morales C, Pinñol T (1998) Relation between the amount of rol Cgene product and indole alkaloid accumulation in Catharanthus roseus transformed root cultures. J Plant Physiol 153:712–718

    Google Scholar 

  • Pinñol MT, Palazón J, Cusido R, Serrano M (1996) Effects of Ri-DNA from Agrobacterium rhizogenes on growth and hyoscyamine production in Datura stramonium root cultures. Bot Acta 109:133–138

    CAS  Google Scholar 

  • Sellés M, Bergonon S, Viladomat F, Bastida J, Codina C (1999) Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids. Plant Cell Rep 18:646–651

    Article  Google Scholar 

  • Stanilova MI, Ilcheva VP, Zagorska NA (1994) Morphogenic potential and in vitro micropropagation of endangered plant species Leucojum aestivumL. and Lilium rhodopaeum Delip. Plant Cell Rep 13:451–453

    Article  CAS  Google Scholar 

  • Trick HN, Finer JJ (1997) SAAT: sonication-assisted Agrobacterium-mediated transformation. Trans Res 6:329–336

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Bruker Daltonics for mass spectroscopy analysis.

Author information

Authors and Affiliations

  1. Groupe S.U.C.R.E.S., U.M.R. 7565 C.N.R.S, Nancy-Université, BP 239, 54506, Nancy-Vandoeuvre, France

    M. F. Diop, F. Chrétien, Y. Chapleur & D. Laurain-Mattar

  2. Laboratoire Agronomie et Environnement, UMR INPL-INRA 1121, ENSAIA, 2 Avenue de forêt de Haye, F-54500, Vandoeuvre les Nancy, France

    A. Hehn, S. Doerper, E. Gontier & F. Bourgaud

  3. Department of Plant Breeding and Seed Science, Agricultural University, Kraków, 31-140, Poland

    A. Ptak

  4. Laboratoire de Botanique,Faculté de Pharmacie, Nancy-Université, 5 rue Albert Lebrun, BP 80403, 54001, Nancy Cedex, France

    M. Henry

Authors
  1. M. F. Diop
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Hehn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Ptak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Chrétien
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Doerper
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. Gontier
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Bourgaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Henry
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Y. Chapleur
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. D. Laurain-Mattar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. Laurain-Mattar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Diop, M.F., Hehn, A., Ptak, A. et al. Hairy root and tissue cultures of Leucojum aestivum L.—relationships to galanthamine content. Phytochem Rev 6, 137–141 (2007). https://doi.org/10.1007/s11101-006-9043-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11101-006-9043-z

Keywords

Search

Navigation