Abstract
The centromere is an essential chromosomal component assembling the kinetochore for chromosome attachment to the spindle microtubules and for directing the chromosome segregation during nuclear division. Kinetochore assembly requires deposition of the centromeric histone H3 variant (CENH3) into centromeric nucleosomes. CENH3 has a variable N-terminal and a more conserved C-terminal part, including the loop1 region of the histone fold domain, which is considered to be critical for centromere targeting. To investigate the structural requirements for centromere targeting, constructs for EYFP-tagged CENH3 of A. lyrata, A. arenosa, Capsella bursa-pastoris, Zea mays and Luzula nivea (the latter with holocentric chromosomes) were transformed into A. thaliana. Except for LnCENH3, all recombinant CENH3 proteins targeted A. thaliana centromeres, but the more distantly related the heterologous protein is, the lower is the efficiency of targeting. Alignment of CENH3 sequences revealed that the tested species share only three amino acids at loop1 region: threonine2, arginine12 and alanine15. These three amino acids were substituted by asparagine, proline and valine encoding sequences within a recombinant EYFP-AtCENH3 construct via PCR mutagenesis prior to transformation of A. thaliana. After transformation, immunostaining of root tip nuclei with anti-GFP antibodies yielded only diffuse signals, indicating that the original three amino acids are necessary but not sufficient for targeting A. thaliana centromeres.
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Acknowledgments
We thank Joachim Bruder and Andrea Kunze for technical assistance, Twan Rutten for assistance with the use of confocal microscope, and Jörg Fuchs for helping with preparation of figures. This work was supported by a grant from the Deutsche Forschungsgemeinschaft to I.S. (SCHU 951/12-1).
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Izabel C.R. Moraes and Inna Lermontova contributed equally to this work.
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Moraes, I.C.R., Lermontova, I. & Schubert, I. Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein. Plant Mol Biol 75, 253–261 (2011). https://doi.org/10.1007/s11103-010-9723-3
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DOI: https://doi.org/10.1007/s11103-010-9723-3