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Improved Yield of Micropropagated Sugarcane Following Inoculation by Endophytic Burkholderia vietnamiensis

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Abstract

During a survey of nitrogen-fixing Burkholderia associated with sugarcane in Tamil Nadu, some endophytes were isolated on PCAT medium. Isolation was based on the use of the selective PCAT medium. Four isolates were studied, all belonging to the genus Burkholderia. One of them, MG43 was consistently more active in reducing acetylene and was identified as Burkholderia vietnamiensis. This isolate was used to inoculate micro-propagated sugarcane plantlets in a comparison with two other diazoptrophs, viz. Gluconacetobacter diazotrophicusT and Herbaspirillum seropedicaeT. Inoculated plants and uninoculated controls were used in a pot experiment followed by two field experiments under different rates of nitrogen fertilisers. MG43 and G. diazotrophicus performed best in sugarcane, their natural host. Biomass increase due to MG43 inoculation reached 20% in the field. Inoculated plants were heavily colonised by the inoculated bacterium (up to 115,000 CFU  g−1 root fresh weight). Inoculation by a combinaison of the three strains performed less well than inoculation by a single MG43 suspension. Ecological implications are discussed, as well as the potential of these bacteria to provide a feasible alternative to higher N fertilisers rates in a low input and long term sustainable rural economy.

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References

  • J Balandreau P Roger (1996) Some comments about a better use of biological Nitrogen fixation in Rice cultivation M Rahman (Eds) Biological Nitrogen Fixation Associated with Rice Production, Kluwer Academic Publish UK 1–12

    Google Scholar 

  • J I Baldani B Pot G Kirchhof E Falsen V L D Baldani F L Olivares B Hoste K Kersters A Hartmann M Gillis J Döbereiner (1996) ArticleTitleEmended description of Herbaspirillum; inclusion of [Pseudomonas] rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3 Int. J. Syst. Bacteriol. 46 802–810 Occurrence Handle1:STN:280:BymA2snotlU%3D Occurrence Handle8782693

    CAS  PubMed  Google Scholar 

  • J I Baldani V L D Baldani L Seldin J Dobereiner (1986) ArticleTitleCharacterisation of Herbaspirillum seropedicae gen. nov. sp. nov., a root associated nitrogen fixing bacterium Int. J. Syst. Bacteriol. 36 86–93 Occurrence Handle1:CAS:528:DyaL28XosFSnuw%3D%3D

    CAS  Google Scholar 

  • Baldani V L D, 1996 Efeito de inoculacao de Herbaspirillum spp. no processo de colonizacao e infeccao de plantas de arroz e ocorrencia caracterizacao parcial de una nova bacteria diazotrofica. Ph.D. thesis, Universidade Federal Rural do Rio de Janeiro

  • R Bally D Thomas-Bauzon T Heulin J Balandreau C Richard J Ley ParticleDe (1983) ArticleTitleDetermination of the most frequent N2-fixing bacteria in a rice rhizosphere Can. J. Microbiol. 29 881–887

    Google Scholar 

  • Y Bashan G Holguin (1997) ArticleTitleAzospirillum-plant relationships: Environmental and physiological advances (1990–1997) Can. J. Microbiol. 43 103–121 Occurrence Handle1:CAS:528:DyaK2sXhsFGmsr0%3D

    CAS  Google Scholar 

  • R Bilal G Rasul J A Qureshi K A Malik (1990) ArticleTitleCharacterisation of Azospirillum and related diazotrophs associated with roots of plants growing in saline sodic soils World J. Microbiol. Biotechnol. 6 46–52 Occurrence Handle10.1007/BF01225354

    Article  Google Scholar 

  • R M Boddey V L D Baldani J I Baldani J Dobereiner (1986) ArticleTitleEffect of inoculation of Azospirillum spp. on nitrogen accumulation by field grown wheat Plant Soil 95 109–121

    Google Scholar 

  • R M Boddey O C Olivera Particlede S Urquiaga V M Reis F L Olivares Particlede V L D Baldani J Dobereiner (1995) ArticleTitleBiological nitrogen fixation associated with sugarcane and rice: Contributions and propects for improvement Plant Soil 174 195–209 Occurrence Handle10.1007/BF00032247 Occurrence Handle1:CAS:528:DyaK2MXotFalsLw%3D

    Article  CAS  Google Scholar 

  • R Boddey S Urquiaga B J R Alves V Reis (2003) ArticleTitleEndophytic nitrogen fixation in sugarcane: Present knowledge and future applications Plant Soil 252 139–149 Occurrence Handle10.1023/A:1024152126541 Occurrence Handle1:CAS:528:DC%2BD3sXksFWgurk%3D

    Article  CAS  Google Scholar 

  • D A Burbage M Sasser (1982) ArticleTitleA medium selective for Pseudomonas cepacia Phytopathol. Abstracts 72 706

    Google Scholar 

  • J Caballero-Mellado L Martínez-Aguilar G Paredes-Valdez P Estrada-de los Santos (2004) ArticleTitleBurkholderia unamae sp nov., a N2-fixing rhizospheric and endophytic species Int. J. Syst. Evol. Microb. 54 1165–1172 Occurrence Handle1:CAS:528:DC%2BD2cXnt1GisLg%3D

    CAS  Google Scholar 

  • V A Cavalcante J Döbereiner (1988) ArticleTitleA new acid-tolerant nitrogen-fixing bacterium associated with sugarcane Plant Soil 108 23–31

    Google Scholar 

  • T Coenye P Vandamme J R Govan J J Lipuma (2001) ArticleTitleTaxonomy and identification of the Burkholderia cepacia complex J Clin Microbiol. 39 3427–3436 Occurrence Handle1:CAS:528:DC%2BD3MXnslKitLo%3D Occurrence Handle11574551

    CAS  PubMed  Google Scholar 

  • J Dobereiner J M Day (1976) Associative symbiosis in tropical grasses, characterization of microorganisms and dinitrogen fixing sites W E Newton C J Nyman (Eds) Proceedings of the International Symposium on Nitrogen Fixation NumberInSeriesVol. 2. Washington State University Press Pullman 518–538

    Google Scholar 

  • J Dobereiner S Urquiaga (1992) ArticleTitleSoil biology and sustainable agriculture An. Acad. Bras. Ci. 84 127–133

    Google Scholar 

  • P Estrada-de-los-Santos R Bustillo-Cristales J Caballero-Mellado (2001) ArticleTitleBurkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution Appl. Environ. Microbiol. 67 2790–2798 Occurrence Handle1:STN:280:DC%2BD3M3pt1ehuw%3D%3D Occurrence Handle11375196

    CAS  PubMed  Google Scholar 

  • P Estrada P Mavingui B Cournoyer F Fontaine J Balandreau J Caballero-Mellado (2002) ArticleTitleA N2-fixing endophytic Burkholderia spp. associated with maize plants cultivated in Mexico Can. J. Microbiol. 48 285–294 Occurrence Handle10.1139/w02-023 Occurrence Handle1:CAS:528:DC%2BD38XjvVGgs7k%3D Occurrence Handle12030700

    Article  CAS  PubMed  Google Scholar 

  • J Fages (1994) Azospirillum inoculation and field experiments Y Okon (Eds) Azospirillum plant associations Boca Raton, Florida CRC press 87–109

    Google Scholar 

  • M Gillis K Kersters B Hoste D Janssens R M Kroppenstedt M P Stephan K R S Teixeira J Döbereiner J DeLey (1989) ArticleTitleAcetobacter diazotrophicus sp. nov. a nitrogen fixing acetic acid bacterium associated with sugar cane Int. J. Syst. Bacteriol. 39 361–364

    Google Scholar 

  • M Gillis V Tran ParticleVan R Bardin M Goor P Hebbar A Willems P Segers K Kerster T Heulin M P Fernandez (1995) ArticleTitlePolyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and the proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam Int. J. Syst. Bacteriol. 45 274–289 Occurrence Handle1:CAS:528:DyaK2MXmtFWhsrc%3D

    CAS  Google Scholar 

  • P Gyaneshwar E K James N Mathan P M Reddy B Reinhold-Hurek J Ladha (2001) ArticleTitleEndophytic colonization of rice by a diazotrophic strain of Serratia marcescen J. Bacteriol. 183 2634–2645 Occurrence Handle10.1128/JB.183.8.2634-2645.2001 Occurrence Handle1:CAS:528:DC%2BD3MXis1artLw%3D Occurrence Handle11274124

    Article  CAS  PubMed  Google Scholar 

  • K Haahtela R Ronkko T Laakso P H Williams T K Korhonen (1990) ArticleTitleRoot-associated Enterobacter and Klebsiella in Poa pratensis: Characterization of an iron-scavenging system and a substance stimulating root hair production Mol. Plant-Microbe Interact. 3 358–365 Occurrence Handle1:CAS:528:DyaK3MXlslyqsbo%3D

    CAS  Google Scholar 

  • A Hartmann J I Baldani G Kirchhof B Assmus P Hutzler N Springer W Ludwig V L D Baldani J Dobereiner (1995) Taxonomic and ecologic studies of diazotrophic rhizosphere bacteria using phylogenetic probes J. Fendrick M Gallo Particledel J Vanderleyden M. Zamaroczy Particlede (Eds) Azospirillum VI and related microorganisms Springer-Verlag Berlin 415–427

    Google Scholar 

  • T Heulin N Omar M Rahman J Balandreau (1991) Some principles for inoculation of rice by Nitrogen-fixing bacteria under field conditions S K Dutta C Sloger (Eds) Biological Nitrogen fixation associated with rice production Oxford and IBH publishing Co New Delhi 221–227

    Google Scholar 

  • T Heulin M Rahman A M N Omar Z Rafidison J C Pierrat J Balandreau (1989) ArticleTitleExperimental and mathematical procedures for comparing efficiencies of rhizosphere N2 fixing bacteria J. Microbiol. Methods 9 163–173 Occurrence Handle10.1016/0167-7012(89)90034-1

    Article  Google Scholar 

  • A Hiraishi (1992) ArticleTitleDirect automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification Lett. Appl. Microbiol. 15 210–213 Occurrence Handle1:CAS:528:DyaK3sXhsVSku7c%3D Occurrence Handle1280147

    CAS  PubMed  Google Scholar 

  • E C Humphries (1956) Mineral components and ash analysis K Peach M V Tracged (Eds) Modern Methods of Plant Analysis Springer-Verlag Berlin 468–502

    Google Scholar 

  • A Isles I Maclusky M Corey R Gold C Prober P Fleming (1984) ArticleTitlePseudomonas cepacia infection in cystic fibrosis: An emerging problem J. Pediatr. 104 206–210 Occurrence Handle1:STN:280:BiuC3MvgvVY%3D Occurrence Handle6420530

    CAS  PubMed  Google Scholar 

  • C Jacoud D Job P Wadoux R Bally (1999) ArticleTitleInitiation of root growth stimulation by Azospirillum lipoferum CRT1 during maize seed germination Can. J. Microbiol. 45 339–342 Occurrence Handle10.1139/cjm-45-4-339 Occurrence Handle1:CAS:528:DyaK1MXksVWju74%3D

    Article  CAS  Google Scholar 

  • T H Jukes C R Cantor (1969) Evolution of protein molecules H N Munro (Eds) Mammalian protein metabolism Academic Press New York, NY 21–132

    Google Scholar 

  • Y Kapulnik J Kigel Y Okon I Nur Y Henis (1981) ArticleTitleEffects of Azospirillum inoculation on some growth parameters and N-content of wheat, sorghum and panicum Plant Soil 61 65–70 Occurrence Handle10.1007/BF02277363

    Article  Google Scholar 

  • I R Kennedy Y T Tchan (1992) ArticleTitleBiological nitrogen fixation in non-leguminous field crops: Recent advances Plant Soil 141 93–118 Occurrence Handle10.1007/BF00011312 Occurrence Handle1:CAS:528:DyaK38Xks1KmsLg%3D

    Article  CAS  Google Scholar 

  • G Kirchhof V M Reis J I Baldani B Eckert J Döbereiner A Hartmann (1997) ArticleTitleOccurrence, physiological and molecular analysis of endophytic diazotrophic bacteria in gramineous energy plants Plant Soil 194 45–55 Occurrence Handle10.1023/A:1004217904546 Occurrence Handle1:CAS:528:DyaK2sXnt1Wju70%3D

    Article  CAS  Google Scholar 

  • S Kumar K Tamura I B Jakobsen M Nei (2001) ArticleTitleMEGA2: Molecular Evolutionary Genetics Analysis Software Bioinformatics 17 1244–1245 Occurrence Handle10.1093/bioinformatics/17.12.1244 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D Occurrence Handle11751241

    Article  CAS  PubMed  Google Scholar 

  • S W Kwon J S Kim I C Park S H Yoon D H Park C K Lim S J Go (2003) ArticleTitlePseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov., and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea Int J Syst Evol Microbiol. 53 21–27 Occurrence Handle1:CAS:528:DC%2BD3sXitVSntrw%3D Occurrence Handle12656147

    CAS  PubMed  Google Scholar 

  • E Mahenthiralingam J Bischof S K Byrne C Radomski J E Davies Y Av-Gay P Vandamme (2000) ArticleTitleDNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, Burkholderia cepacia genomovars I and III J. Clin. Microbiol. 38 3165–3173 Occurrence Handle1:CAS:528:DC%2BD3cXmvFyiurc%3D Occurrence Handle10970351

    CAS  PubMed  Google Scholar 

  • T Murashige F A Skoog (1962) ArticleTitleRevised medium for rapid growth and bioassays with tobacco tissue culture, Physiol Plant. 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm

    CAS  Google Scholar 

  • R Muthukumarasamy G Revathi C Lakshminarasimhan (1999) ArticleTitleDiazotrophic bacterial association of sugarcane Tropical agriculture 76 171–178

    Google Scholar 

  • Y Okon C Labandera-Gonzalez (1994) ArticleTitleAgronomic applications of Azospirillum: An evaluation of 20 years of world-wide field inoculation Soil Biol. Biochem. 26 1591–1601 Occurrence Handle10.1016/0038-0717(94)90311-5 Occurrence Handle1:CAS:528:DyaK2MXisFWrtbY%3D

    Article  CAS  Google Scholar 

  • A L M Oliveira S Urquiaga J Döbereiner J I Baldani (2002) ArticleTitleThe effect of inoculating endophytic N2-fixing bacteria on micropropagated sugarcane plants Plant and Soil 242 205–215 Occurrence Handle10.1023/A:1016249704336 Occurrence Handle1:CAS:528:DC%2BD38XltFKisb8%3D

    Article  CAS  Google Scholar 

  • N Omar O Berge S N Shalaan J L Hubert T Heulin J Balandreau (1992) ArticleTitleInoculation of Rice with Azospirillum brasilense in Egypt. Results of five different trials between 1985 and 1990 Symbiosis 13 281–289

    Google Scholar 

  • C Pallud V Viallard J Balandreau P Normand G Grundmann (2001) ArticleTitleCombined use of a specific probe and PCAT medium to study Burkholderia in soil J. Microbiol. Meth. 47 25–34 Occurrence Handle10.1016/S0167-7012(01)00287-1 Occurrence Handle1:CAS:528:DC%2BD3MXmvVKktLg%3D

    Article  CAS  Google Scholar 

  • J A R Pereira V A Cavalcante J I J Baldani Dobereiner (1988) ArticleTitleField inoculation of Sorghum and rice with Azospirillum spp. and Herbaspirillum seropedicae Plant Soil 110 269–274 Occurrence Handle10.1007/BF02226807

    Article  Google Scholar 

  • B Reinhold-Hurek T Hurek M Gillis B Hoste M Vacanneyt K Kersters J deLey (1993) ArticleTitleAzoarcus gen. nov., nitrogen-fixing proteobacteria associated with roots of kallar grass (Leptochloa fusca (L.) Kunth), and description of two species, Azoarcus indigens sp.nov., and Azoarcus communis sp.nov Int. J. Syst. Bacteriol. 43 574–584

    Google Scholar 

  • V M Reis F B Olivares A L M Oliveira F B Reis Junior J I Baldani J Dobereiner (1999) ArticleTitleTechnical approaches to inoculate micropropagated sugarcane plants by Acetobacter diazotrophicus Plant Soil 206 205–211

    Google Scholar 

  • V M Reis F L Olivares J Döbereiner (1994) ArticleTitleImproved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat, World J. Microbiol. Biotechnol. 10 101–104

    Google Scholar 

  • V M Reis P Estrada Los Santos Particlede S Tenorio-Salgado J Vogel M Stoffels S Guyon P Mavingui V L D Baldani M Schmid J I Baldani J Balandreau A Hartmann J Caballero-Mellado (2004) ArticleTitleBurkholderia tropica sp nov: A novel nitrogen-fixing, plant-associated bacterium Int. J. Syst. Evol. Microbiol. 54 IssueID6 2155–2162 Occurrence Handle1:CAS:528:DC%2BD2cXhtFGjs7fI Occurrence Handle15545451

    CAS  PubMed  Google Scholar 

  • Robertson S, Kingston G, Dart P J and Brown S 2001 Occurence of endophytic bacteria in Australian sugarcane. 8th International Symposium on Nitrogen Fixation with Non-Legumes, Sydney, December 3–7. Abstract book. p.140

  • N Saitou M Nei (1987) ArticleTitleThe neighbor-joining method: A new method for reconstructing phylogenetic trees Mol. Biol. Evol. 4 406–425 Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015

    CAS  PubMed  Google Scholar 

  • S Sarig Y Okon A Blum (1992) ArticleTitleEffect of Azospirillum brasilense inoculation on growth dynamics and hydraulic conductivity of Sorghum bicolor roots J. Plant Nutr. 15 805–819

    Google Scholar 

  • M Sevilla R H Burris N Gunapala C Kennedy (2001) ArticleTitleComparison of benefit to sugarcane plant growth and 15N2 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and nif- mutant strains Mol. Plant-Microbe Interact. 14 IssueID3 358–366 Occurrence Handle1:CAS:528:DC%2BD3MXhsVKgsb8%3D Occurrence Handle11277433

    CAS  PubMed  Google Scholar 

  • M Sievers H G Schlegel J Caballero Mellado J Dobereiner W Ludwig (1998) ArticleTitlePhylogenetic identification of two major nitrogen-fixing bacteria associated with sugarcane Syst. Appl. Microbiol. 21 IssueID4 505–508 Occurrence Handle1:STN:280:DyaK1M7itlyqsQ%3D%3D Occurrence Handle9924818

    CAS  PubMed  Google Scholar 

  • J Sreenivasan T V Sreenivasan (1984) ArticleTitleIn vitro propagation of Saccharum officinarum and Sclerostachya fusca hybrid Theor. Appl. Genet. 67 171–174 Occurrence Handle10.1007/BF00317026

    Article  Google Scholar 

  • V Trân ParticleVan O Berge J Balandreau S Ngo Kê T Heulin (1996) ArticleTitleIsolement et activité nitrogénasique de Burkholderia vietnamiensis, bactérie fixatrice d’azote associée au riz (Oryza sativa L) cultivé sur un sol sulfaté acide du Viêt-nam Agronomie 16 479–491

    Google Scholar 

  • V Trân ParticleVan P Mavingui O Berge J Balandreau T Heulin (1994) ArticleTitlePromotion de croissarice du riz inoculé par une bactérie fixatrice d’azote, Burkholderia vietnamiensis, isolée d’un sol sulfaté acide du Vietnam Agronomie 14 697–707

    Google Scholar 

  • V Trân ParticleVan O Berge S Ngô Kê J Balandreau T Heulin (2000) ArticleTitleRepeated beneficial effects of rice inoculation with a strain of Burkholderia vietnamiensis on early and late yield components in low fertility sulphate acid soils of Vietnam Plant Soil 218 273–284

    Google Scholar 

  • T Ueda Y Suga N Yahiro T Matsuguchi (1995) ArticleTitleRemarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences J.␣Bacteriol. 177 1414–1417 Occurrence Handle1:CAS:528:DyaK2MXktVSnu7k%3D Occurrence Handle7868622

    CAS  PubMed  Google Scholar 

  • S Urquiaga K H S Cruz R M Boddey (1992) ArticleTitleContribution of nitrogen fixation to sugarcane nitrogen−15 and nitrogen balance estimates Soil Sci. Soc. Am. J. 56 105–114

    Google Scholar 

  • P Vandamme B Holmes M Vancanneyt T Coenye B Hoste R Coopman H Revets S Lauwers M Gillis K Kersters J R W Govan (1997) ArticleTitleOccurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov Int. J. Syst. Bacteriol. 47 1188–1200 Occurrence Handle1:STN:280:ByiH2sfgtFA%3D Occurrence Handle9336927

    CAS  PubMed  Google Scholar 

  • K Vermis T Coenye E Mahenthiralingam H J Nelis P Vandamme (2002) ArticleTitleEvaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex J. Med. Microbiol. 51 1–4

    Google Scholar 

  • K Vermis T Coenye J J LiPuma E Mahenthiralingam J H Nelis P Vandamme (2004) ArticleTitleProposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov Int. J. Syst. Evol.Microbiol. 54 689–691 Occurrence Handle1:CAS:528:DC%2BD2cXlt1enurY%3D Occurrence Handle15143009

    CAS  PubMed  Google Scholar 

  • V Viallard I Poirier B Cournoyer J Haurat S Wiebkin K Ophel-Keller J Balandreau (1998) ArticleTitleBurkholderia graminis sp. nov. a novel species of rhizospheric Burkholderia and reassessment of Pseudomonas phenazinium, Ps. pyrrocinia and Ps. glathei into Burkholderia Int. J. Syst. Bacteriol. 48 549–563 Occurrence Handle1:CAS:528:DyaK1cXkt1ahtLs%3D Occurrence Handle9731297

    CAS  PubMed  Google Scholar 

  • Y Yamada K Hoshino T Ishikawa (1997) ArticleTitleThe phylogeny of acetic acid bacteria based on the partial sequences of 16S ribosomal RNA: The elevation of the subgenus Gluconacetobacter to the generic level Biosci. Biotechnol. Biochem. 61 1244–1251 Occurrence Handle1:CAS:528:DyaK2sXlvFeqtL8%3D Occurrence Handle9301103

    CAS  PubMed  Google Scholar 

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  1. Main Biocontrol Res. Lab., Goodwill avenue, Vembakkam, Chengalpattu-1, India

    Munusamy Govindarajan, Ramachandran Muthukumarasamy & Gopalakrishnan Revathi

  2. Ecologie Microbienne, UMR CNRS 5557, Bât Gregor Mendel, Université Cl. Bernard Lyon 1, 43 Bd du 11 Novembre, F-69622, Villeurbanne cedex, France

    Jacques Balandreau

  3. PR Group of Institutions, Tanjavur, India

    Cunthipuram Lakshminarasimhan

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  1. Munusamy Govindarajan
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  2. Jacques Balandreau
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Correspondence to Jacques Balandreau.

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Govindarajan, M., Balandreau, J., Muthukumarasamy, R. et al. Improved Yield of Micropropagated Sugarcane Following Inoculation by Endophytic Burkholderia vietnamiensis. Plant Soil 280, 239–252 (2006). https://doi.org/10.1007/s11104-005-3223-2

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