Abstract
Background and aims
Reliance on nitrogen (N) fertilizers to maintain crop productivity requires a thorough understanding of the transformation of this nutrient within the soil-plant system. Organic matter input from a mixture of crop residues, such intercrop systems, influence N transformations differently compared to sole crops. We tested the hypothesis that N mineralization and immobilization differ between cereal-legume intercrops and sole crops.
Methods
A short-term experiment using 15N isotopic pool dilution was conducted in 2007 and 2012 in maize (Zea mays L.) and soybean (Glycine max L. Merr.) sole crops and 1:2 (1 row maize:2 rows soybean) and 2:3 (two rows maize:3 rows soybean) intercrops. Soil characteristics, gross mineralization and immobilization, and net immobilization to a 10 cm depth were quantified.
Results
Soil characteristics (pH, bulk density, soil organic carbon (C), total N, and C:N) were not significantly different (P < 0.05) among treatments, but differed significantly (P < 0.05) between years (2007 vs. 2012). Soil NH4 +-N was significantly lower (P < 0.05) in the maize sole crop. Gross N mineralization, immobilization and net immobilization, were significantly different (P < 0.05) among treatments and between years. Relative NH4 +-N immobilization was significantly different (P < 0.05) among treatments and between years, showing the lowest values in the intercrops. The amount of NH4 +-N mineralized per day was significantly greater (P < 0.05) in the 2:3 intercrop and was significantly different (P < 0.05) between years in the 2:3 intercrop. Residence time of NH4 +-N was significantly longer (P < 0.05) in the soybean sole crop and 1:2 intercrop followed by the 2:3 intercrop and the maize sole crop.
Conclusions
Intercropping contributed to the long-term immobilization of N and therefore was a more sustainable land-management practice than sole cropping. The adoption of cereal-legume intercrops will curb our currently growing reliance on N fertilizers.
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References
Aber JD (1992) Nitrogen cycling and nitrogen saturation in temperate forest ecosystems. Trends Ecol Evol 7:220–223
Accoe F, Boeckx P, Busschaert J, Hofman G, Van Cleemput O (2004) Gross N transformation rates and net N mineralization rates related to the C and N contents of soil organic matter fractions in grassland soils of different age. Soil Biol Biochem 36:2075–2087
Alvarez CR, Alvarez R, Grigera MS, Lavado RS (1998) Associations between organic matter fractions and the active soil microbial biomass. Soil Biol Biochem 30:767–773
Andrade FH (1995) Analysis of growth and yield of maize, sunflower and soybean growth at Balcarce, Argentina. Field Crop Res 42:1–12
Aparicio V, Costa JL (2007) Soil quality indicators under continuous cropping systems in the Argentinean Pampa. Soil Tillage Res 96:155–165
Arihara J, Srinivasan A (2001) Significance of nutrient uptake mechanisms in cropping systems. In: Noriharu AE, Arihara J, Okada K, Srinivasan A (eds) Plant nutrient acquisition-new perspectives. Springer, Japan, pp 487–503
Baggs EM, Chebii J, Ndufa JK (2006) A short-term investigation of trace gas emissions following tillage and no-tillage of agroforestry residues in western Kenya. Soil Tillage Res 90:69–76
Barrios E, Buresh RJ, Sprent JI (1996) Nitrogen mineralization in density fractions of soil organic matter from maize and legume cropping systems. Soil Biol Biochem 28:1459–1465
Bedoussac L, Justes E, Journet ÉP, Hauggaard-Nielsen H, Naudin C, Corre-Hellou G, Prieur L, Jensen (2014) Intercropping – applying ecological principles for improved nitrogen use efficiency in organic farming systems. In: Bellon S, Penvern S (eds) Organic farming, Prototype for sustainable agricultures. Springer Science, Dordrecht, pp 47–64
Bengston G, Bengston P, Mansson KF (2003) Gross nitrogen mineralization-, immobilization-, and nitrification rates as a function of soil C/N ratio and microbial activity. Soil Biol Biochem 35:143–154
del Blanco CM, Stoops G (2007) Genesis of pedons with discontinuous argillic horizons in the Holcene loess mantle of the southern Pampean landscape, Argentina. J S Am Earth Sci 23:30–45
Bremner JM (1965) Inorganic forms of nitrogen. In: Black CA (ed) Methods of soil analysis part II: chemical and microbiological properties. American Society of Agronomy, Madison, pp 1149–1176
Burger M, Jackson LE (2003) Microbial immobilization of ammonium and nitrate in relation to ammonification and nitrification rates in organic and conventional cropping systems. Soil Biol Biochem 25:29–36
Chapagain T, Riseman A (2014) Barley-pea intercropping: effects on land productivity, carbon and nitrogen transformations. Field Crop Res 166:18–25
Chapman K, Whittaker JB, Heal OW (1988) Metabolic and faunal activity in litter of tree mixtures compared with pure stands. Agric Ecosyst Environ 24:33–40
Davidson EA, Hart SC, Shanks CA, Firestone MK (1991) Measuring gross nitrogen mineralization, immobilization, and nitrification by 15N isotopic pool dilution in intact soil cores. J Soil Sci 42:349–355
Domínguez G, Diovisalvi N, Studdert G, Monterubbianesi M (2009) Soil organic C and N fractions under continuous cropping with contrasting tillage systems on Mollisols of the southeastern Pampas. Soil Tillage Res 102:93–100
Dyer L, Oelbermann M, Echarte L (2012) Soil carbon dioxide and nitrous oxide emissions during the growing season from temperate maize-soybean intercrops. J Plant Nutr Soil Sci 175:394–400
Echarte L, Della Maggiora A, Cerrudo D, Gonzalez VH, Abbate P, Cerrudo A, Sadra VO, Calviño P (2011) Yield response to plant density of maize and sunflower intercropped with soybean. Field Crop Res 121:423–429
Flavel TC, Murphy DV (2006) Carbon and nitrogen mineralization rates after application of organic amendments to soil. J Environ Qual 35:183–193
Frimpong KA, Yawson DO, Agyarko K, Baggs EM (2012) N2O emission and mineral N release in a tropical Acrisol incorporated with mixed cowpea and maize residues. Agronomy 2:167–186
Galloway JN, Aber JD, Erisman JW, Seitzinger SP, Howarth RW, Cowling EB, Cosby BJ (2003) The nitrogen cascade. Bioscience 53:341–356
Griffin TS (2007) Estimates of gross transformation rates of dairy manure N using 15N pool dilution. Commun Soil Sci Plant Anal 38:1451–1465
Hart SC, Nason GE, Myrold DD, Perry DA (1994) Dynamics of gross nitrogen transformations in an old-growth forest: the carbon connection. Ecology 75:880–891
Hauggaard-Nielsen H, Jensen ES (2001) Evaluating pea and barley cultivars for complementarity in intercropping at different levels of soil N availability. Field Crop Res 72:185–196
Hauggaard-Nielsen H, Mundus S, Jensen ES (2012) Grass-clover undersowing affects nitrogen dynamics in a grain legume-cereal arable cropping system. Field Crop Res 136:23–31
Hood R, Bautista E, Heiling M (2003) Gross mineralization and plant N uptake from animal manures under non-N limiting conditions, measured using the 15N isotope dilution technique. Phytochem Rev 2:113–119
INTA (Instituto Nacional de Tecnología Agropecuaria) (1979) Carta de Suelos de la República Argentina. Hoja: 3757–31, Balcarce., Instituto Nacional de Tecnología Agropecuaria, Secretaría de Agricultura, Ganadería y Pesca, Buenos Aires, Argentina
Jamont M, Piva G, Fustec J (2013) Sharing N resources in the early growth of rapeseed intercropped with faba been: does N transfer matter? Plant Soil 371:641–653
Kirkham D, Bartholomew WV (1954) Equations for following nutrient transformations in soil, utilizing tracer data. Soil Sci Soc Am J 18:33–34
Kutsch WL, Bahn M, Heinemeyer A (2012) Soil carbon relations: an overview. In: Kutsch WL, Bahn M, Heinemeyer A (eds) Soil carbon dynamics-an integrated methodology. Cambridge University Press, Cambridge, pp 1–15
Liang B, Zhao W, Yang X, Zhou J (2013) Fate of nitrogen-15 as influenced by soil and nutrient management history in a 19-year wheat-maize experiment. Field Crop Res 144:124–134
Li W, Li L, Sun J, Guo T, Zhang F, Bao X, An P, Tang C (2005) Effects of intercropping and nitrogen application on nitrate present in the profile of an Orthic Anthrosol in northwest China. Agric Ecosyst Environ 105:483–491
Li L, Sun J, Zhang F, Li X, Yang S, Rengel Z (2001) Wheat-maize or wheat-soybean strip intercropping I. Yield advantage and interspecific interactions on nutrients. Field Crop Res 71:123–137
Luxhoi J, Bruun S, Stenberg B, Breland TA, Jensen LS (2006) Prediction of gross and net nitrogen mineralization-immobilization turnover from respiration. Soil Sci Soc Am J 70:1121–1128
Malhi SS, Moulin AP, Johnston AM, Kutcher HR (2008) Short-term and long-term effects of tillage and crop rotation on soil physical properties, organic C and N in a black Chernozem in northeastern Saskatchewan. Can J Soil Sci 88:273–282
Mary B, Recous S, Darwis D, Robin D (1996) Interactions between decomposition of plant residues and nitrogen cycling in soil. Plant Soil 181(7):71–82
Matusso JMM, Mugwe JN, Mucheru-Muna M (2014) Effects of different maize (Zea mays L.) – soybean (Glycine max (L.) Merrill) intercropping patterns on soil mineral-N, N-uptake and soil properties. Afr J Agric Res 9:42–55
Mendham DS, Kumaraswamy S, Balasundran M, Sandaran KV, Corbeels M, Grove TS, O’Connel AM, Rance SJ (2004) Legume cover cropping effects on early growth and soil nitrogen supply in eucalypt plantations in south-western India. Biol Fertil Soils 39:375–382
Midwood AJ, Boutton TW (1998) Soil carbonate decomposition by acid has little effect on δ13C of organic matter. Soil Biol Biochem 30:1301–1307
Monaghan R (1995) Errors in estimates of gross rates of nitrogen mineralization due to non- uniform distributions of 15N label. Soil Biol Biochem 27:855–859
Mulvaney RL, Khan SA, Stevens WB, Mulvaney CS (1997) Improved diffusion methods for determination of inorganic nitrogen in soil extracts and water. Biol Fertil Soils 24:413–420
Nieder R, Benbi DK (2008) Anthropogenic activities and soil carbon and nitrogen. In: Nieder R, Benbi DK (eds) Carbon and nitrogen in the terrestrial environment. Springer, Germany, pp 161–218
Oelbermann M, Regehr A, Echarte L (2015) Changes in soil characteristics after six seasons of cereal-legume intercropping in the southern Pampa. Geoderma Reg 4:100–107
Oelbermann M, Echarte L (2011) Evaluating soil carbon and nitrogen dynamics in recently established maize-soyabean inter-cropping systems. Eur J Soil Sci 62:35–41
Pappa VA, Rees RM, Walker RL, Baddeley JA, Watson CA (2011) Nitrous oxide emissions and nitrate leaching in an arable rotation resulting from the presence of an intercrop. Agric Ecosyst Environ 141:153–165
Pelzer E, Bazot M, Makowski D, Corre-Hellou G, Naudin C, Al Rifai M, Baranger E, Bedoussac L, Biarnes V, Boucheny P, Carrouee B, Dorvillez D, Foissy D, Gaillard B, Guichard L, Mansard MC, Omon B, Prieur L, Yverginaux M, Justes E, Jeuffroy MH (2012) Pea-wheat intercrops in low-input conditions combine high economic performances and low environmental impact. Eur J Agron 40:39–52
Powlson DA, Barraclough D (1993) Mineralization and assimilation in soil-plant systems. In: Knowles R, Blackburn TH (eds) Nitrogen isotope techniques. Academic Press, Inc., San Diego, pp 209–242
Redin M, Recous S, Aita C, Dietrich G, Caitan Skilaude A, Hytalo Ludgke W, Schmatz R, Giancomini SJ (2014) How the chemical composition and heterogeneity of crop residue mixtures decomposing at the soil surface affects C and N mineralization. Soil Biol Biochem 78:65–75
Reents HJ, Möller K (2000) Effects of different green manure catch crops grown after peas on nitrate dynamics in soils and on yield and quality of subsequent potatoes and wheat. Proceedings of the 13th International IFOAM Scientific Conference. IFOAM, Zurich, pp 73–75
Regehr A (2013) Evaluation of maize and soybean intercropping on soil quality and nitrogen transformations in the Argentine Pampa. M.Sc. Thesis, University of Waterloo
Sakala WD, Cadisch G, Giller KE (2000) Interactions between residues of maize and pigonpea and mineral N fertilizers during decomposition and N mineralization. Soil Biol Biochem 32:679–688
Sanchez JE, Paul EA, Wilson TC, Smeen J, Harwood RR (2002) Corn root effects on the nitrogen-supplying capacity of a conditional soil. Agronomy 94:391–396
Sharma AR, Behera UK (2009) Recycling of legume residues for nitrogen economy and higher productivity in maize (Zea mays)-wheat (Triticum aestivum) cropping system. Nutr Cycl Agroecosyst 83:197–210
Sørensen P, Jensen ES (1991) Sequential diffusion of ammonium and nitrate from soil extracts to a polytetraethylene trap for 15N determination. Anal Chim Acta 252:201–203
Steel GD, Torrie JH, Dickey DA (1997) Principles and procedures of statistics: a biometrical approach. McGraw-Hill, New York
Studdert G, Echeverría HE (2000) Crop rotations and nitrogen fertilization to manage soil organic carbon dynamics. Soil Sci Soc Am J 64:1496–1503
Tracy BF, Frank DS (1998) Herbivore influence on soil microbial biomass and nitrogen mineralization in a northern grassland ecosystem: Yellowstone National Park. Oecologia 114:556–562
Tolon-Becerra A, Tourn M, Botta GF, Lastra-Bravo X (2011) Effects of different tillage regimes on soil compaction, maize (Zea mays L.) seedling emergence and yields in the eastern Argentinean Pampas region. Soil Tillage Res 117:184–190
Vachon KE, Oelbermann M (2011) Crop residue input and decomposition in a temperate maize-soybean intercrop system. Soil Sci 175:157–163
Vandermeer J (1992) The ecology of intercropping. Cambridge University Press, Cambridge
Verchot LV, Holmes Z, Groffman PM, Lovett GM (2001) Gross vs net rates of N mineralization and nitrification as indicators of functional differences between forest types. Soil Biol Biochem 33:1889–1901
Vervaet H, Boeckx P, Boko AMCV, Cleemput O, Hofman G (2004) The role of gross and net N transformation processes and NH4 + and NO3 − immobilization in controlling the mineral N pool of a temperate mixed deciduous forest soil. Plant Soil 264:349–357
Videla C Del Carmen (2004) Mineralização bruta de nitrogênio em um Mollisol do sudeste da Provincia de Buenos Aires (Argentina). Ph.D. Thesis, Centro de Energía Nuclear na Agricultura, Piracicaba, Brazil
Zaman M, Di HJ, Cameron KC, Frampton CM (1999) Gross nitrogen mineralization and nitrification rates and their relationships to enzyme activities and the soil microbial biomass in sols treated with dairy effluent and ammonium fertilizer at different water potentials. Biol Fertil Soils 29:178–186
Zhang F, Li L (2003) Using competitive and facilitative interactions in intercropping systems enhances crop productivity and nutrient-use efficiency. Plant Soil 284:305–312
Acknowledgments
Funding for this study was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Canadian Foundation for Innovation (CFI). This work was supported by the University of Waterloo, the Instituto Nacional de Tecnologia Agropecuaria (INTA), Universidad Nacional de Mar del Plata, the National Research Council of Argentina (CONICET), and a scholarship to A. Regehr from NSERC, the Inter-American Institute for Co-operation on Agriculture (IICA-Canada) and the University of Waterloo. We also thank J. Schimel for his constructive editorial comments, and the anonymous reviewers that helped improve this manuscript.
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Regehr, A., Oelbermann, M., Videla, C. et al. Gross nitrogen mineralization and immobilization in temperate maize-soybean intercrops. Plant Soil 391, 353–365 (2015). https://doi.org/10.1007/s11104-015-2438-0
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DOI: https://doi.org/10.1007/s11104-015-2438-0