Abstract
The diet rich in fruits and vegetables reduces the risk of metabolic syndrome, including diabetes development by various mechanisms of action, mainly due to the presence of polyphenolic compounds. Extracts from different conifer species are known to be a rich source of various polyphenols. In the present study we elucidated the in vitro mechanism of anti-diabetic activity of silver fir (Abies alba) wood and bark extracts and compared their activity to non-coniferous sweet chestnut wood extract and standardized maritime pine bark extract. Extracts and lignans were tested for their inhibitory activity of enzymes involved in the regulation of blood glucose in vitro. The ability of extracts to protect against oxidative stress in high glucose environment was tested on mouse myoblast cell line. Silver fir wood and bark extracts were shown to be effective inhibitors of α-glucosidase, α-amylase and dipeptidyl peptidase 4, three enzymes involved in the regulation of blood glucose levels. Coniferous extracts also showed protection against oxidative stress generated in high glucose environment. Lignans, particularly pinoresinol diglucoside, isolariciresinol and secolariciresinol were shown to be important contributors of antihyperglycemic activity through inhibition of dipeptidyl peptidase 4. This corroborates previously published in vivo results on blood glucose level obtained with silver fir wood extract and supports the use of silver fir wood and bark extracts as food supplements or functional foods in borderline diabetes.
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Abbreviations
- DPP4:
-
Dipeptidyl peptidase 4
- ROS:
-
Reactive oxygen species
References
Hameed I, Masoodi SR, Mir SA, Nabi M, Ghazanfar K, Ganai BA (2015) Type 2 diabetes mellitus: from a metabolic disorder to an inflammatory condition. World J Diabetes 6:598–612. https://doi.org/10.4239/wjd.v6.i4.598
Kanat M, DeFronzo RA, Abdul-Ghani MA (2015) Treatment of prediabetes. World J Diabetes 6:1207–1222. https://doi.org/10.4239/wjd.v6.i12.1207
Schulze MB, Hu FB (2005) Primary prevention of diabetes: what can be done and how much can be prevented? Annu Rev Public Health 26:445–467. https://doi.org/10.1146/annurev.publhealth.26.021304.144532
Ceriello A (2005) Postprandial hyperglycemia and diabetes complications: is it time to treat? Diabetes 54:1–7. https://doi.org/10.2337/diabetes.54.1.1
Dhital S, Lin AH-M, Hamaker BR, Gidley MJ, Muniandy A (2013) Mammalian mucosal α-glucosidases coordinate with α-amylase in the initial starch hydrolysis stage to have a role in starch digestion beyond glucogenesis. PLoS One 8:e62546. https://doi.org/10.1371/journal.pone.0062546
Lacroix IME, Li-Chan ECY (2012) Evaluation of the potential of dietary proteins as precursors of dipeptidyl peptidase (DPP)-IV inhibitors by an in silico approach. J Funct Foods 4:403–422. https://doi.org/10.1016/j.jff.2012.01.008
Bakirel T, Bakirel U, Keles OU, Ulgen SG, Yardibi H (2008) In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits. J Ethnopharmacol 116:64–73. https://doi.org/10.1016/j.jep.2007.10.039
Vijayakumar M, Govindarajan R, Rao GM, Rao Ch V, Shirwaikar A, Mehrotra S, Pushpangadan P (2006) Action of Hygrophila auriculata against streptozotocin-induced oxidative stress. J Ethnopharmacol 104:356–361. https://doi.org/10.1016/j.jep.2005.09.030
Wongsa P, Chaiwarit J, Zamaludien A (2012) In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand. Food Chem 131:964–971. https://doi.org/10.1016/j.foodchem.2011.09.088
Xiao JB, Hogger P (2015) Dietary polyphenols and type 2 diabetes: current insights and future perspectives. Curr Med Chem 22:23–38. https://doi.org/10.2174/0929867321666140706130807
Bahadoran Z, Mirmiran P, Azizi F (2013) Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J Diabetes Metab Disord 12:43. https://doi.org/10.1186/2251-6581-12-43
Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI (2005) Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition 21:756–761. https://doi.org/10.1016/j.nut.2004.10.014
Liu X, Wei J, Tan F, Zhou S, Würthwein G, Rohdewald P (2004) Antidiabetic effect of Pycnogenol® French maritime pine bark extract in patients with diabetes type II. Life Sci 75:2505–2513. https://doi.org/10.1016/j.lfs.2003.10.043
Benković ET, Grohar T, Žigon D, Švajger U, Janeš D, Kreft S, Štrukelj B (2014) Chemical composition of the silver fir (Abies alba) bark extract Abigenol® and its antioxidant activity. Ind Crop Prod 52:23–28. https://doi.org/10.1016/j.indcrop.2013.10.005
Drevenšek G, Lunder M, Tavčar Benković E, Mikelj A, Štrukelj B, Kreft S (2015) Silver fir (Abies alba) trunk extract protects Guinea pig arteries from impaired functional responses and morphology due to an atherogenic diet. Phytomedicine 22:856–861. https://doi.org/10.1016/j.phymed.2015.06.004
Willför S, Nisula L, Hemming J, Reunanen M, Holmbom B (2004) Bioactive phenolic substances in industrially important tree species. Part 2: knots and stemwood of fir species. Holzforschung 58:650-659. https://doi.org/10.1515/HF.2004.119
Tavčar Benković E, Žigon D, Mihailović V, Petelinc T, Jamnik P, Kreft S (2017) Identification, in vitro and in vivo antioxidant activity, and gastrointestinal stability of lignans from silver fir (Abies alba) wood extract. J Wood Chem Technol 37:467–477. https://doi.org/10.1080/02773813.2017.1340958
Rejc T, Petrič U, Debeljak J, Bremec T, Ferk P, Lunder M, Roškar I, Štrukelj B, Kreft S (2016) Zmes naravnih polifenolov iz lesa bele jelke za zmanjšanje postprandialne glukoze. Slovenia, Urad RS za intelektualno lastnino. Patent SI 24984 A 30(11):2016
Roškar I, Štrukelj B, Lunder M (2016) Screening of phenolic compounds reveals inhibitory activity of nordihydroguaiaretic acid against three enzymes involved in the regulation of blood glucose level. Plant Foods Hum Nutr 71:88–89. https://doi.org/10.1007/s11130-016-0530-0
Schäfer A, Högger P (2007) Oligomeric procyanidins of French maritime pine bark extract (Pycnogenol®) effectively inhibit α-glucosidase. Diabetes Res Clin Pract 77:41–46. https://doi.org/10.1016/j.diabres.2006.10.011
Peterson J, Dwyer J, Adlercreutz H, Scalbert A, Jacques P, McCullough ML (2010) Dietary lignans: physiology and potential for cardiovascular disease risk reduction. Nutr Rev 68:571–603. https://doi.org/10.1111/j.1753-4887.2010.00319.x
Debeljak J, Ferk P, Čokolič M, Zavratnik A, Tavčar Benković E, Kreft S, Štrukelj B (2016) Randomised, double blind, cross-over, placebo and active controlled human pharmacodynamic study on the influence of silver fir wood extract (Belinal) on post-prandial glycemic response. Pharmazie 71:566–569. https://doi.org/10.1691/ph.2016.6658
Hu M (2007) Commentary: bioavailability of flavonoids and polyphenols: call to arms. Mol Pharm 4:803–806. https://doi.org/10.1021/mp7001363
Xu Z, Ju J, Wang K, Gu C, Feng Y (2014) Evaluation of hypoglycemic activity of total lignans from Fructus Arctii in the spontaneously diabetic Goto-Kakizaki rats. J Ethnopharmacol 151:548–555. https://doi.org/10.1016/j.jep.2013.11.021
Brownlee M (2001) Biochemistry and molecular cell biology of diabetic complications. Nature 414:813–820. https://doi.org/10.1038/414813a
Kim A, Lee W, Yun JM (2017) Luteolin and fisetin suppress oxidative stress by modulating sirtuins and forkhead box O3a expression under in vitro diabetic conditions. Nutr Res Pract 11(5):430–434. https://doi.org/10.4162/nrp.2017.11.5.430
Xu X, Jiang H, Liu H, Zhang W, Xu X, Li Z (2012) The effects of galanin on dorsal root ganglion neurons with high glucose treatment in vitro. Brain Res Bull 87(1):85–93. https://doi.org/10.1016/j.brainresbull.2011.10.012
Kim YJ, Kim YA, Yokozawa T (2011) Pycnogenol modulates apoptosis by suppressing oxidative stress and inflammation in high glucose-treated renal tubular cells. Food Chem Toxicol 49:2196–2201. https://doi.org/10.1016/j.fct.2011.06.012
Shashi B, Jaswant S, Madhusudana RJ, Kumar SA, Nabi QG (2006) A novel lignan composition from Cedrus deodara induces apoptosis and early nitric oxide generation in human leukemia Molt-4 and HL-60 cells. Nitric Oxide 14:72–88. https://doi.org/10.1016/j.niox.2005.09.009
Hošek J, Šmejkal K (2016) Flavonoids as anti-inflammatory agents. In: Parnham MJ (ed) Compendium of inflammatory diseases. Springer Basel, Basel, pp 482–497. https://doi.org/10.1007/978-3-0348-0620-6_19-1
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M. Lunder, I. Roškar and B. Štrukelj are co-authors of Patent SI 24984 A.
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Lunder, M., Roškar, I., Hošek, J. et al. Silver Fir (Abies alba) Extracts Inhibit Enzymes Involved in Blood Glucose Management and Protect against Oxidative Stress in High Glucose Environment. Plant Foods Hum Nutr 74, 47–53 (2019). https://doi.org/10.1007/s11130-018-0698-6
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DOI: https://doi.org/10.1007/s11130-018-0698-6