Abstract
Apprehension over exhaustion of fossil fuels and global warming, due to increasing amounts of CO2, has generated a lot of attention for the subject of renewable energy. Renewable energy has an intermittency problem and its output fluctuates depending on natural conditions. Biohydrogen is one of the promising renewable energy sources. Hydrogen produced by photosynthetic bacteria depends on the intensity of light irradiation and also fluctuates with the daily variation of sunlight. The co-culture system of dark-fermentative and photosynthetic bacteria is one solution for reducing the dependency of hydrogen production on light intensity. Because these two strains of bacteria have different processes of hydrogen production, it is possible to combine different outputs so far as the co-culture system works well. This study performed hydrogen production by the co-culture system composed of agar gels embedded with both dark-fermentative bacteria, Clostridium butyricum MIYAIRI, and photosynthetic bacteria, Rhodobacter sphaeroides RV, under a fluctuating light-irradiation. The time-course of hydrogen production was determined for the different conditions of co-culture in the mixing ratios of the two bacterial strains and light-irradiation patterns. As a result, the co-culture system succeeded in producing hydrogen exceeding that in the case of a single culture system and improved its stability against light fluctuation. Hydrogen production by the co-culture system would be applicable to the reduction of intermittency in renewable energies.
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References
M. Ni, D.Y.C. Leung, M.K.H. Leung, K. Sumathy, An overview of hydrogen production from biomass. Fuel Process. Technol. 87, 461–472 (2006)
D.B. Nelson, M.H. Nehrir, C. Wang, Unit sizing and cost analysis of stand-alone hybrid wind/PV/fuel cell power generation system. Renew. Energy 31, 1641–1656 (2006)
A.N. Celik, Optimisation and techno-economic analysis of autonomous photovoltaic–wind hybrid energy systems in comparison to single photovoltaic and wind systems. Energy Convers. Manag. 43, 2453–2468 (2002)
A. Perez-Navarro, D. Alfonso, C. Alvarez, F. Ibanez, C. Sanchez, I. Segura, Hybrid biomass-wind power plant for reliable energy generation. Renew. Energy 35, 1436–1443 (2010)
H. Argun, F. Kargi, Bio-hydrogen production by different operational modes of dark and photo-fermentation: an overview. Int. J. Hydrogen Energy 36, 7443–7459 (2011)
I. Karube, T. Matsunaga, S. Tsuru, S. Suzuki, Continous hydrogen production by immobilized whole cells of Clostridium butyricum. Biochem. Biophys. Acta 444, 338–343 (1976)
I. Karube, N. Urano, T. Matsunaga, S. Suzuki, Hydrogen production from glucose by immobilized growing cells of Clostridium butyricum. Eur. J. Appl. Microbiol. Biotechnol. 16, 5–9 (1982)
J. Miyake, X.Y. Mao, S. Kawamura, Photoproduction of hydrogen from glucose by a co-culture of a photosynthetic bacterium and Clostridium butyricum. J. Ferment. Technol. 62, 531–535 (1984)
J. Ding, B.F. Liu, N.Q. Ren, D.F. Xing, W.Q. Guo, J.F. Xu, G.J. Xie, Hydrogen production from glucose by co-culture of Clostridium Butyricum and immobilized Rhodopseudomonasfaecalis RLD-53. Int. J. Hydrogen Energy 34, 3647–3652 (2009)
B.F. Liu, N.Q. Ren, J. Tang, J. Ding, W.Z. Liu, J.F. Xu, G.L. Cao, W.Q. Guo, G.J. Xie, Bio-hydrogen production by mixed culture of photo- and dark-fermentation bacteria. Int. J. Hydrogen Energy 35, 2858–2862 (2010)
F. Kargi, S. Ozmihci, Effects of dark/light bacteria ratio on bio-hydrogen production by combined fed-batch fermentation of ground wheat starch. Biomass Bioenergy 34, 869–874 (2010)
H. Argun, F. Kargi, I.K. Kadpan, Effects of the substrate and cell concentration on bio-hydrogen production from ground wheat by combined dark and photo-fermentation. Int. J. Hydrogen Energy 34, 6181–6188 (2009)
H. Argun, F. Kargi, Effects of light source, intensity and lighting regime on bio-hydrogen production from ground wheat starch by combined dark and photo-fermentations. Int. J. Hydrogen Energy 35, 1604–1612 (2010)
Y. Asada, M. Tokumoto, Y. Aihara, M. Oku, K. Ishimi, T. Wakayama, J. Miyake, M. Tomiyama, H. Kohno, Hydrogen production by co-cultures of Lactobacillus and a photosynthetic bacterium, Rhodobactersphaeroides RV. Int. J. Hydrogen Energy 31, 1509–1513 (2010)
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Takagi, D., Okamura, S., Tanaka, K. et al. Characterization of hydrogen production by the co-culture of dark-fermentative and photosynthetic bacteria. Res Chem Intermed 42, 7713–7722 (2016). https://doi.org/10.1007/s11164-016-2656-y
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DOI: https://doi.org/10.1007/s11164-016-2656-y