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Evidence for antioxidants consumption in the coronary blood of patients with an acute myocardial infarction

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Abstract

Blood flow arrest and reperfusion during myocardial infarction (MI) cause myocyte and endothelium injury through oxidative stress and inflammation, both of which involve Reactive Oxygen Species (ROS) and peroxides that consume antioxidant defenses. The aim of this study was to determine whether serum from the occluded coronary vessel has impaired anti-oxidative defenses as compared to serum from aortic blood or from the periphery of healthy controls. Forty-seven patients (44 men) were included for study. Inclusion criteria were chest pain, ST elevation, and cardiac troponin increase. A photoreaction producing a standardized amount of singlet oxygen (1O2), an excited form of oxygen, was performed in serum samples obtained during primary percutaneous coronary intervention (PCI). Immediately after laser light delivery to 5 % sera containing 5 µg/mL rose bengal, dichloro-dihydro-fluorescein (DCFH) was added and its post-oxidation transformation into the fluorescent DCF, was recorded. At least 5 h after the start of symptoms, the mean secondary ROS production after 1O2 delivery was increased in coronary sera (p < 0.001), but in aortic blood remained similar to that of healthy controls. The peak troponin value correlated with DCF fluorescence throughout the interval between symptoms onset and PCI. A high fluorescence was associated with a higher risk of MACE. These results show that oxidants secondary to 1O2 are increased in occluded vessels during AMI in parallel to c-troponin, demonstrating that antioxidants are consumed. A O2 increase during reperfusion would thus extend the damage resulting from IDM necrosis. The effect of conditioning during PCI could be studied using the described method.

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Abbreviations

1O2 :

Singlet oxygen

AMI:

Acute myocardial infarction

AUC:

Area under the curve

DCF:

Dichlorofluorescein (oxidized, fluorescent)

DCF-DA:

Dichlorofluorescein diacetate

DCFH:

Dichlorofluorescein (reduced, non fluorescent)

MACE:

Major adverse cardiac events

RB:

Rose bengal

ROS:

Reactive oxygen species

SD:

Standard deviation

SOS:

Secondary reactive oxygen species and peroxides

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Acknowledgments

Acknowledgments to the Fondation pour la Recherche Médicale, the Ligue Nationale contre le Cancer, the Etablissement Français du Sang and Dr J Ashton-Chess for English translation.

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Authors and Affiliations

  1. Institut du Thorax, Service d’Hémodynamique, Laënnec Hospital, 44093, Nantes, France

    Patrice Guerin

  2. Biochemistry, Laënnec Hospital, 44093, Nantes, France

    Edith Bigot

  3. Photobiologie des Cancers, Laser Department, Laënnec Hospital, 44093, Nantes, France

    Thierry Patrice

Authors
  1. Patrice Guerin
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  2. Edith Bigot
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  3. Thierry Patrice
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Correspondence to Thierry Patrice.

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Guerin, P., Bigot, E. & Patrice, T. Evidence for antioxidants consumption in the coronary blood of patients with an acute myocardial infarction. J Thromb Thrombolysis 35, 41–47 (2013). https://doi.org/10.1007/s11239-012-0774-y

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  • DOI: https://doi.org/10.1007/s11239-012-0774-y

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