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Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)

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Abstract

Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 μM thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 μM 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA3) to conversion medium containing 5 μM 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries.

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Abbreviations

ABA:

Abscisic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

BA:

6-Benzyladenine

2iP:

2-Isopentyl adenine

GA3 :

Gibberellic acid

TDZ:

Thidiazuron

References

  • Bohanec B, Marijana J, Alojz I, Branka J (1995) Studies of gynogenesis in onion (Allium cepa L.) induction procedures and genetic analysis of regenerents. Plant Sci 104:215–224

    Article  CAS  Google Scholar 

  • Bonga JM (1982) Vegetative propagation in relation to juvenility, maturity and rejuvenation. In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Martinus Nijhoff/Dr W Junk, The Hague, pp 387–412

    Google Scholar 

  • Branton RL, Blake J (1983) Development of organized structures in callus derived from explants of Cocos nucifera L. Ann of Bot 52:673–678

    Google Scholar 

  • Buffard-Morel J, Verdeil J-L, Pannetier C (1988) Vegetative propagation of coconut palm through somatic embryogenesis, obtention of plantlets from leaf expla. In: Durand G, Bobichon L, Florent J (eds) Proceedings of the 8th International Biotechnology Symposium, Paris, p 117

  • Chalupa V (1990) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tilia cordata Mill.). Plant Cell Tiss Organ Cult 9:398–401

    CAS  Google Scholar 

  • Chan JL, Saenz L, Talavera C, Hornung R, Robert M, Oropeza C (1998) Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep 17:515–521

    Article  CAS  Google Scholar 

  • Compton ME (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tiss Organ Cult 37:217–242

    Google Scholar 

  • Conger BV, Novak FJ, Afza R, Erdelsky K (1987) Somatic embryogenesis from cultured leaf segments of Zea mays. Plant Cell Rep 6:345–347

    Article  Google Scholar 

  • Custodio L, Carneiro MF, Romano A (2005) Microsporogenesis and anther culture in carob tree (Ceratonia siliqua L.). Sci Hort 104:65–77

    Article  CAS  Google Scholar 

  • Dransfield J, Uhl NW (1986) An outline classification of palms. Principes 30:3–11

    Google Scholar 

  • Eeckhaut T, Werbrouck S, Dendauw J, Bockstaele EV, Debergh P (2001) Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. Plant Cell Tiss Organ Cult 67:181–189

    Article  CAS  Google Scholar 

  • Fernando SC, Gamage CKA (2000) Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos nucifera L.). Plant Sci 151:193–198

    Article  PubMed  CAS  Google Scholar 

  • Fernando SC, Verdeil J-L, Hocher V, Weerakoon LK, Hirimburegama K (2003) Histological analysis of plant regeneration from plumule explants of Cocos nucifera L. Plant Cell Tiss Organ Cult 72:281–284

    Article  Google Scholar 

  • Fraser LG, Harvey CF (1986) Somatic embryogenesis from anther-derived callus in two Actinidia species. Sci Hort 29:335–346

    Article  Google Scholar 

  • Ghosh B, Sen S (1991) Plant regeneration through somatic embryogenesis from spear callus culture of Asparagus cooperi Baker. Plant Cell Rep 9:667–670

    Article  CAS  Google Scholar 

  • Gmitter FG, Moore GA (1986) Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: Embryo production, germination and plant survival. Plant Cell Tiss Organ Cult 6:139–147

    Article  CAS  Google Scholar 

  • Griffis JL, Litz RE (1997) Advances in the in vitro morphogenesis of several coconut (Cocos nucifera L.) tissues in Florida. International Cashew & Coconut Conference, Tanzania, pp 349–357

  • Gurel S, Gurel E, Kaya Z (2000) Double haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L). Plant Cell Rep 19:1155–1159

    Article  CAS  Google Scholar 

  • Hatanaka T, Arakawa O, Yasuda T, Uchida N, Yamaguchi T (1991) Effect of plant growth regulators on somatic embryogenesis in leaf cultures of Coffea canephora. Plant Cell Rep 10:179–182

    Article  CAS  Google Scholar 

  • Hidaka T, Kajiura I (1988) Plantlet differentiation from callus protoplast induced from citrus embryos. Sci Hort 34:85–92

    Article  Google Scholar 

  • Hornung R (1995) Initiation of callogenesis in coconut palm (Cocos nucifera L.). In: Oropeza C, Howard FW, Ashburner GR (eds) Lethal yellowing: research and practical aspects. Kluwer, Dordrecht, pp 203–215

    Google Scholar 

  • Hornung R, Verdeil J-L (1999) Somatic embryogenesis in coconut from immature inflorescence explants. In: Oropeza C, Verdeil J-L, Ashburner GR, Cardena R, Santamaria JM (eds) Current advances in coconut biotechnology. Kluwer, Dordrecht, pp 297–308

    Google Scholar 

  • Huetteman C, Preece J (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Organ Cult 33:105–119

    Article  CAS  Google Scholar 

  • Karunaratne S, Periyapperuma K (1989) Culture of immature embryos of coconut (Cocos nucifera L.): callus proliferation and somatic embryogenesis. Plant Sci 62:247–253

    Article  Google Scholar 

  • Kochba J, Button J, Spiegel-Roy P, Bornman CH, Kochba M (1974) Stimulation of rooting of Citrus embryoids by gibberellic acid and adenine sulphate. Ann Bot 38:795–802

    CAS  Google Scholar 

  • Kott LS, Kasha KJ (1984) Initiation and morphological development of somatic embryoids from barley cell cultures. Can J Bot 62:1245–1249

    Article  Google Scholar 

  • Kunitake H, Mii M (1990) Somatic embryogenesis and plant regeneration from protoplasts of asparagus (Asparagus officinalis L.). Plant Cell Rep 8:706–710

    Article  CAS  Google Scholar 

  • Levi A, Sink KC (1991) Asparagus somatic embryogenesis: Production in suspension culture and conversion to plantlets on solidified medium as influenced by carbohydrate regime. Plant Cell Tiss Organ Cult 31:115–122

    Article  Google Scholar 

  • Lu C-Y, Chandler SF, Vasil IK (1984) Somatic embryogenesis and plant regeneration from cultured immature embryo of rye (Secale cereale L.). J Plant Physiol 115:237–244

    CAS  Google Scholar 

  • Luthar Z, Bohanec B (1999) Induction of direct somatic embryogenesis in onion (Allium cepa L.) using a two step flower or ovary culture. Plant Cell Rep 18:797–802

    Article  CAS  Google Scholar 

  • Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): evidence for somatic embryogenesis. Protoplasma 110:95–105

    Article  Google Scholar 

  • Pannetier C, Buffard-Morel J (1982) Premiers résultats concernant la production d’embryons somatiques à partir de tissus foliaires de cocotier, Cocos nucifera L. Oleagineux 37:349–354

    Google Scholar 

  • Parrott WA, Merkle SA, Williams EG (1991) Somatic embryogenesis: potential for use in propagation and gene transfer systems. In: Murray DR (ed) Advanced methods in plant breeding and biotechnology. CAB International, UK, pp 158–200

    Google Scholar 

  • Peixe A, Barroso J, Potes A, Pais MS (2004) Induction of haploid morphogenic calluses from in vitro cultured anthers of Prunus armeniaca cv. ‘Harcot’. Plant Cell Tiss Organ Cult 77:35–41

    Article  CAS  Google Scholar 

  • Perera PIP, Hocher V, Verdeil J-L, Doulbeau S, Yakandawala DMD, Weerakoon LK (2007) Unfertilized ovary: a novel explant for coconut (Cocos nucifera L.) somatic embryogenesis. Plant Cell Rep 26:21–28

    Article  PubMed  CAS  Google Scholar 

  • Perez-Nunez MT, Chan JL, Saenz L, Gonzalez T, Verdeil JL, Oropeza C (2006) Improved somatic embryogenesis from coconut (Cocos nucifera L.) plumule explants cultured in vitro. In Vitro Cell Dev Biol-Plant 42:37–43

    Article  Google Scholar 

  • Radojevic L (1985) Tissue culture of maize Zea mays (Cudu) I. Somatic embryogenesis in the callus tissue. J Plant Physiol 119:435–441

    Google Scholar 

  • Ruth SK, Stephen LS, John CB (1993) Ovule culture of sweet potato (Ipomoea batatas) and closely related species. Plant Cell Tiss Organ Cult 32:77–82

    Article  Google Scholar 

  • Rybczynski JJ, Zdunczy KW (1986) Somatic embryogenesis and plant regeneration in the genus Secale I. Somatic embryogenesis and organogenesis from cultured immature embryos of five wild species of rye. Theor Appl Genet 73:267–271

    Article  Google Scholar 

  • SAS Institute Inc. (1999) SAS/STAT user’s guide, version 7–1. SAS Institute, Cary

    Google Scholar 

  • Sivadas P, Kothari SL, Chandra N (1990) High frequency embryoid and plantlet formation from tissue cultures of the finger millet (Eleusine coracana L.). Plant Cell Rep 9:93–96

    Article  Google Scholar 

  • Spiegel-Roy P, Vardi A (1984) Citrus. In: Ammirato PV, Evans DR, Sharp WR, Yamada Y (eds) Handbook of plant cell culture, vol 3. Macmillan, New York, pp 355–372

    Google Scholar 

  • Srinivasan C, Vasil IK (1986) Plant regeneration from protoplasts of sugarcane (Saccharum officinarum L.). J Plant Physiol 126:41–48

    CAS  Google Scholar 

  • Tang F, Tao Y, Zhao T, Wang G (2006) In vitro production of haploid and double haploid plants from pollinated ovaries of maize (Zea mays). Plant Cell Tiss Organ Cult 84:233–237

    Article  Google Scholar 

  • Thomas V, Rao PS (1985) In vitro propagation of oil palm (Elaeis giineensis Jacq. var. tenera) through somatic embryogenesis in leaf-derived callus. Current Sci 54:184–185

    Google Scholar 

  • Tisserat B (1979) Propagation of date palm (Phoenix dactylifera L.) in vitro. J Exp Bot 30:1275–1283

    Article  CAS  Google Scholar 

  • Tisserat B (1982) Factors involved in the production of plantlets from date palm callus cultures. Euphytica 31:201–214

    Article  Google Scholar 

  • Van Creij MGM, Kerckhoffs DMFJ, De Bruijn SM, Vreugdenhil D, Van Tuyl JM (2000) The effect of medium composition on ovary-slice culture and ovule culture in interspecific Tulipa gesneriana crosses. Plant Cell Tiss Organ Cult 60:61–67

    Article  Google Scholar 

  • Van Tuyl JM, Van Dien MP, Van Creij MGM, Van Kleinwee TCM, Franken J, Bino RJ (1991) Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci 74:115–126

    Article  Google Scholar 

  • Verdeil J-L, Huet C, Grosdemange F, Buffard-Morel J (1994) Plant regeneration from cultured immature inflorescence of coconut (Cocos nucifera L.): evidence for somatic embryogenesis. Plant Cell Rep 13:218–221

    Article  CAS  Google Scholar 

  • Weerakoon LK, Vidhanaarachchi VRM, Fernando SC, Fernando A, Gamage CKA (2002) Increasing the efficiency of embryo culture technology to promote coconut germplasm collecting and exchange in Sri Lanka. In: Engelmann F, Batugal P, Oliver J (eds) Coconut embryo in vitro culture: part II. IPGRI-APO, Malaysia, pp 27–40

    Google Scholar 

  • Weigel RC, Hughes KW (1985) Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants. Plant Cell Tiss Organ Cult 5:151–162

    Article  Google Scholar 

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Acknowledgments

We are thankful to J.D.J.S. Kularatne, Biometry Division, Coconut Research Institute, Lunuwila, Sri Lanka for his assistance in statistical analysis. Special thanks go to Mr. Kapila Prasanna, Tissue Culture Division, Coconut Research Institute, Sri Lanka, for careful attention in sample collection.

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Authors and Affiliations

  1. Tissue Culture Division, Coconut Research Institute, Lunuwila, 61150, Sri Lanka

    P. I. P. Perera, V. R. M. Vidhanaarachchi, T. R. Gunathilake & L. K. Weerakoon

  2. Department of Botany, University of Peradeniya, Peradeniya, Sri Lanka

    D. M. D. Yakandawala

  3. Institute for Research and Development (IRD), UMR 1098 BEPC, IRD, 911 Av. Agropolis, BP 64501, 34394, Montpellier Cedex 1, France

    V. Hocher

  4. CIRAD, TA40/02 Avenue Agropolis, 34398, Montpellier Cedex 5, France

    J. L. Verdeil

  5. Postgraduate Institute of Science, University of Peradeniya, Peradeniya, Sri Lanka

    P. I. P. Perera & D. M. D. Yakandawala

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  1. P. I. P. Perera
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  2. V. R. M. Vidhanaarachchi
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  3. T. R. Gunathilake
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  6. J. L. Verdeil
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  7. L. K. Weerakoon
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Correspondence to P. I. P. Perera.

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Perera, P.I.P., Vidhanaarachchi, V.R.M., Gunathilake, T.R. et al. Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.). Plant Cell Tiss Organ Cult 99, 73–81 (2009). https://doi.org/10.1007/s11240-009-9577-z

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  • DOI: https://doi.org/10.1007/s11240-009-9577-z

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