Abstract
Spontaneous shoot regeneration was observed from Agrobacterium rhizogenes-induced hairy roots of Plumbago indica when these were incubated in liquid MS medium for a period of 3 weeks under continuous light. Insertion of the rolB gene in putative transformed plants was confirmed by PCR and sequencing. Transformed plants grown for a period of 1 week on solid MS medium containing 0.5 mg l−1 6-benzyladenine and then transplanted to growth regulator-free medium showed better overall growth than control plants. Transformed plants had a higher root bio-biomass and an increased plumbagin content relative to non-transformed plants.
Abbreviations
- BA:
-
6-Benzyladenine
- HPLC:
-
High performance liquid chromatography
- IAA:
-
Indole acetic acid
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Acknowledgments
The authors thank the Director, Bose Institute for providing the necessary facilities and funding through an Institutional Fellowship to MG. The immensely helpful comments of the anonymous reviewers is thankfully acknowledged.
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11240_2010_9702_MOESM1_ESM.eps
Supplementary Fig. 1: HPLC chromatograms, a. standard plumbagin, b. transformed plant grown in 0.5 mg l−1 BA for 1 week, c. non-transformed plant. (EPS 589 kb)
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Gangopadhyay, M., Chakraborty, D., Bhattacharyya, S. et al. Regeneration of transformed plants from hairy roots of Plumbago indica . Plant Cell Tiss Organ Cult 102, 109–114 (2010). https://doi.org/10.1007/s11240-010-9702-z
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DOI: https://doi.org/10.1007/s11240-010-9702-z