Abstract
Jatropha curcas microshoots excised from well-established multiple shoot cultures of CSMCRI-1 were used as microscions. Different age microscion (6 to18-month-old shoots) and rootstock (15 to 90-day-old) were used for grafting. Among all the different combinations, 18-month-old scions and 60-day-old rootstocks developed grafts with 58.4 % success rate. The grafting efficiency was further increased by treating scion and rootstock with 0.05 mg/L BAP (6-benzylaminopurine) and 0.05 mg/L ZN (zeatin). BAP treated scion grafted on 60 days old seedlings established early union. Graft union was dressed with tissue paper wetted with 0.1 % Carbemedozo and Macrinite (CM-75) and 400 mg/L amoxicillin-clavulanic acid for infection free graft. Dressing significantly increased the rate of survival (97 %) of the grafted plants. 97 % microshoots were successfully rooted via grafting. Grafting altered the plant architecture and made them dwarf. The net photosynthetic rate of grafted plants was higher than non-grafted plants. The seed yield of grafted plants was significantly higher than cuttings and seedling.
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Acknowledgments
CSIR-CSMCRI Communication No. 079/2015. The financial support received from Ministry of New and Renewable Energy, and Council of Scientific and Industrial Research, New Delhi, India (project GAP1060 and MLP0014) are thankfully acknowledged.
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Singh, A., Agrawal, P.K. Jatropha curcas micrografting modifies plant architecture and increases tolerance to abiotic stress: grafting modifies the architecture of Jatropha curcas . Plant Cell Tiss Organ Cult 128, 243–246 (2017). https://doi.org/10.1007/s11240-016-1098-y
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DOI: https://doi.org/10.1007/s11240-016-1098-y