Abstract
Linum persicum contains significant amount of lignans as podophyllotoxin that may show therapeutic effects against several types of metastatic cancers such as breast cancer. The aim of this study was to establish the cell culture of L. persicum for lignans production and evaluation for anti-carcinogenic activity on MCF7 cell line. Also, lignan contents of the cell, callus, and plantlets and also in different parts including; capsule, leave, stem and root of the wild plants were determined using HPLC technique. The highest podophyllotoxin in capsules and pinoresinol in leaf and root organs were observed. Shoot extract of the in-vitro plantlets have greater cytotoxic effect on breast cancer cells (IC50 = 5 µg/ml) and leads to significant raise in ratio of Bax/Bcl-2 value = 1.42 and Rbl1 gene expression that are well-known as apoptotic inducing agents. In conclusion, due to cytotoxic activity of in vitro plantlet extracts on MCF7 cell line with limited side-effects; the corresponding extracted component could be led to therapeutic approaches in future.
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Change history
07 February 2018
There was an spelling error in the seventh author’s first name in the initial online publication. The original article has been corrected.
Abbreviations
- PTOX:
-
Podophyllotoxin
- FW:
-
Fresh weight
- MPTOX:
-
Methoxy podophyllotoxin
- NAA:
-
1-Naphthalene acetic acid
- Kin:
-
Kinetin
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Acknowledgements
This work was supported financially by Tarbiat Modares University and Stem Cell Technology Research Center.
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This research paper was accomplished with the collaboration of authors. ME performed the experiments, analyzed data and wrote the manuscript. MS designed the study, supervised the study and wrote the manuscript. FM-T analyzed data. HH-A wrote the manuscript. MY advised the study. AJ collected plant samples. SH and MS advised the study and edited the manuscript.
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Communicated by Sergio J. Ochatt.
The original version of this article has been revised: The seventh author's first name has been corrected.
A correction to this article is available online at https://doi.org/10.1007/s11240-018-1386-9.
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Esfandiari, M., Sharifi, M., Mohamadyar-Toupkanlou, F. et al. Optimization of cell/tissue culture of Linum persicum for production of lignans derivatives including Podophyllotoxin. Plant Cell Tiss Organ Cult 133, 51–61 (2018). https://doi.org/10.1007/s11240-017-1360-y
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DOI: https://doi.org/10.1007/s11240-017-1360-y