Skip to main content
SpringerLink
Log in

Ovulatory Response and Embryo Yield in Jakhrana Goats Following Treatments with PMSG and FSH

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Superovulatory response and embryo production efficacy were investigated in adult (age 2–4 years, average body weight: 27–43 kg) cycling Jakhrana goats (n = 15) under semi-arid environmental conditions of India by administering different superovulatory regimens. Goats were reared under semi-intensive system of management in established farm conditions. To synchronize oestrus, a luteolytic dose of carboprost tromethamine (Upjohn, UK) was administered intramuscularly to all does at the dose rate of 5μg per kg body weight in a double dose schedule with an interval of 11 days. For superovulation, 750 IU of PMSG (Folligon, Intervet, Boxmeer, Holland) per goat was administered intramuscularly 24 h before administering a second dose of luteolytic agent in five does (treatment 1). FSH (Sigma, St. Louis, MO, USA) 12.50 IU per goat was administered intramuscularly in a decreasing daily dose schedule (2.50, 2.50; 1.875, 1.875; 1.25, 1.25; 0.625, 0.625) at 12 h intervals over four days, initiated 48 h before administering second dose of carboprost tromethamine in 5 does (treatment 2). FSH (Super-Ov, Ausa Intern, USA) was administered at a uniform dose rate of 8.33 units per goat intramuscularly at 24 h intervals over three consecutive days (total dose was 25 units), initiated 48 h before administering a second dose of carboprost tromethamine in 5 does (treatment 3). To synchronize ovulation in responders, human chorionic gonadotrophin (hCG, Chorulon, Intervet) was injected intramuscularly at a dose rate of 500 IU in each goat on the day of oestrus appearance. Goats were laparotomized 72–82 h following the onset of synchronized oestrus and their genitalia were flushed using a standard collection procedure. Variability (p > 0.05) in superovulatory response (number of established corpora lutea) was observed: FSH (Sigma), 11.8± 2.9; FSH (Super-Ov), 11.6±4.5; PMSG (Intervet), 8.4±2.3. A similar pattern was reflected in mean embryo and transferable embryo recovery, respectively (p > 0.05): FSH (Sigma), 8.0±1.8, 5.2±1.7; FSH (Super-Ov), 6.6±2.4, 5.4±2.4; PMSG, 5.8±1.9, 3.8±2.2. In PMSG-treated does, comparatively more unfertilized ova or retarded embryos were recovered than in FSH-treated does. The superiority of FSH preparations over PMSG was reflected in terms of total and transferable embryo production (p > 0.05). On average, five transferable embryos (excellent and good quality) were recovered per doe treated with FSH of either source. The mean ova/embryo recovery was satisfactory (55–68%). Results indicated that Jakhrana goats can be superovulated for embryo production using FSH of either source to augment productivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FSH:

follicle-stimulating hormone

PMSG:

pregnant mare's serum gonadotrophin

IU:

international unit

hCG:

human chorionic gonadotrophin

i.m.:

intramuscular(ly)

i.v.:

intravenous(ly)

References

  • Agrawal, K.P., Mongha, I.V. and Bhattacharyya, N.K., 1982. Collection and transfer of embryos in goats: surgical method. Indian Veterinary Journal, 59, 298–303

    Google Scholar 

  • Amoah, E.A. and Gelaye, S., 1990. Super ovulation, synchronization and breeding of does. Small Ruminant Research, 3, 63–72

    Google Scholar 

  • Armstrong, D.T., Pfitzner, A.P., Warnes, G.M., Ralph, M.M. and Seamark, R.F., 1983a. Endocrine responses of goats after induction of superovulation with PMSG and FSH. Journal of Reproduction and Fertility, 67, 395–401

    CAS  Google Scholar 

  • Armstrong, D.T., Pfitzner, A.P., Warnes, G.M., Ralph, M.M. and Seamark, R.F., 1983b. Superovulation treatments and embryo transfer in Angora goats. Journal of Reproduction and Fertility, 67, 403–410

    CAS  Google Scholar 

  • Bakshi, S.A., Doijode, S.V., Pargaonkar, M.D. and Pargaonkar, D.R., 1995. Studies on synchronization of oestrus, superovulation and surgical embryo recovery in goats. Proceedings of National Symposium on Embryo Transfer and Genetic Engineering Technologies in Small Ruminants, Central Institute for Research on Goats, Makhdoom, Mathura, India, 7–8 May, 1995 (Abstract no. A-5)

  • Baril, G. and Vallet, J., 1990. Time of ovulation in dairy goats induced to super ovulate with porcine follicle stimulating hormone during and out of the breeding season. Theriogenology, 34, 303–311

    Article  Google Scholar 

  • Bonia, K.K., 1992. A study on embryo transfer in Assam local goats, (PhD thesis, Assam Agricultural University, Khanapara, Guwahati, Assam, India)

  • Delgadillo, J.A. and Malpaux, B., 1996. Reproduction of goats in tropics and sub tropics. Proceedings of the 6th International Conference on Goats, Beijing, China, 1996, 785–793

  • Goel, A.K. and Agrawal, K.P., 1990. Superovulation and embryo collection in Jamunapari goats. Theriogenology, 33, 232 (Abstract)

    Article  Google Scholar 

  • Goel, A.K. and Agrawal, K.P., 2000. Oestrus synchronization in Jakhrana goats following administration of prostaglandin and intra-vaginal sponges. Indian Journal of Animal Sciences, 70, 714–715

    CAS  Google Scholar 

  • Goel, A.K. and Agrawal, K.P., 2003. Ovulation in Jakhrana goats native to tropical climates. Small Ruminant Research, 50, 209–212

    Article  Google Scholar 

  • Goel, A.K., Tyagi, S. and Agrawal K.P., 1993. Superovulation and embryo recovery in Jamunapari goats using FSH of equine origin. Indian Journal of Animal Reproduction, 14, 18–19

    Google Scholar 

  • Gogai, A.K., Borgohain, B.N., Deka, B.C. and Chakravarthy, P., 2001. A study on the super ovulatory response and embryo recovery in Assam local goats. Indian Journal of Animal Reproduction, 22, 26–29

    Google Scholar 

  • Greyling, J.P.C., NestVander, M., Schwalbach, L.M.J. and Muller, T., 2002. Super ovulation and embryo transfer in South African Boer and indigenous Feral goats. Small Ruminant Research, 43, 45–51

    Article  Google Scholar 

  • Hafez, E.S.E., 1987. Embryo transfer, IVF and genetic engineering. In: E.S.E. Hafez (ed.), Reproduction in Farm Animals, 5th edn, (Lea and Febiger, Philadelphia)

    Google Scholar 

  • Harvey, W.A., 1990. User's guide for LSMLMW and mix MDL. PC-2 version, Mixed model least squares and maximum likehood computer programme. (ARS, USA)

  • Jabbour, H.N. and Evans, G., 1991. Ovarian and endocrine responses of Merino ewes following treatment with PMSG or GnRH or PMSG anti serum. Animal Reproduction Science, 24, 259–270

    CAS  Google Scholar 

  • Kiessling, A.A., Hughes, W.H. and Blankevoort, M.R., 1986. Super ovulation and embryo transfer in the dairy goat. Journal of American Veterinary Medical Association, 188, 829–832

    CAS  Google Scholar 

  • Lee, C.S., Fang, N.Z., Koo, D.B., Lee, Y.S., Zheng, G.D., Oh, K.B., Youn, W.S., Han, Y.M., Kim, S.J., Lim, J.H., Shin, S.T., Jin, S.W., Lee, K.S., Ko, J.H., Koo, J.S., Park, C.S., Lee, K.S., Yoo, O.J. and Lee, K.K., 2000. Embryo recovery and transfer for the production of transgenic goats from Korean Native strain (Capra hircus aegargus). Small Ruminant Research, 37, 57–63

    Article  Google Scholar 

  • Linder, G.M. and Wright, R.W. Jr., 1983. Bovine embryo morphology and evaluation. Theriogenology, 20, 407–411

    Google Scholar 

  • Mahmood, S., Koul, G.L. and Biswas, J.C., 1989. Superovulatory responses in Pashmina goats. Veterinary Record, 29, 117

    Google Scholar 

  • Mahmood, S., Koul, G.L. and Biswas, J.C., 1991. Comparative efficacy of FSH-P and PMSG on superovulation in Pashmina goats. Theriogenology, 35, 1191–1196

    Article  CAS  Google Scholar 

  • Mishra, O.P., Gawande, P.G., Nema, R.K. and Tiwari, S.K., 2004. Estrus behaviour and super ovulatory response in Black Bengal goats (Capra hircus) following administration of prostaglandin and gonadotropins. Asian-Australasian Journal of Animal Sciences, 17, 1374–1377

    CAS  Google Scholar 

  • Moore, N.W., 1982. Egg transfer in sheep and goats. In: C.E. Adams (ed.), Mammalian Egg Transfer, (CRC Press, Boca Raton, FL), 119–131

    Google Scholar 

  • Nowshari, M.A., Beckers, J.F. and Holtz, W., 1995. Superovulation of goats with purified p-FSH supplemented with defined amount of p-LH. Theriogenology, 43, 797–802

    Article  CAS  Google Scholar 

  • Nowshari, M.A., Yus-Waiti Eti., Puls-Kleingeld, M. and Holtz, W., 1992. Superovulation in peri-pubertal and adult goats treated with PMSG or p-FSH. Pre Conference Proceedings of the V International Conference on Goats, New Delhi, India, 2–8 March, 1992, vol. I

  • Nuti, L.C., Minhas, B.S., Baker, W.C., Capehart, J.S. and Marrack, P., 1987. Super ovulation and recovery of zygotes from Nubian and Alpine dairy goats. Theriogenology, 28, 481–488

    Article  Google Scholar 

  • Pendelton, R.J., Youngs, C.R., Rorie, R.W., Memon, M.A. and Godke, R.A., 1992. Follicle stimulating hormone versus pregnant mare serum gonadotropin for super ovulation of dairy goats. Small Ruminant Research, 8, 217–224

    Google Scholar 

  • Rai, B. and Singh, M.K., 2004. Rearing practices of Jakhrana goat in farmer's flock. Indian Journal of Small Ruminants, 10, 33–35

    Google Scholar 

  • Rai, B., Tiwari, S. and Khan, B.U., 2001. The Jakhrana goat in India. Animal Genetic Resources Information, FAO, Rome, 29, 41–48

    Google Scholar 

  • Rajkhowa, N.K., Baruah, B., Chakraborty, P., Sarmah, B.C. and Talukder, S.C., 1992. Study of stage of embryo at 72 hours post-mating in HCG and PMSG: HCG treated local goats of Assam. Indian Journal of Animal Physiology, 1(1), 15–19

    Google Scholar 

  • Rosniha, Y., Jainudeen, M.R. and Nihayah, M., 1992. Super ovulation and egg recovery in goats in the tropics. Veterinary Record, 130, 93–97

    Google Scholar 

  • Sukhato, P., Ratanapaskon and Eiamvitayakom, 1991. Superovulatory response to pregnant mare serum gonadotropins in Sannen goats. In: S. Saithanoo and B.W. Norton (eds), Goat Production in the Asian Humid Tropics, Proceedings of an International Seminar, Hat Yai, Thialand, 28–31 May, 1991, 196–200

  • Thilager, S., Kathiresan, D., Padmanabhan, V.O. and Dewan Muthu, M.S., 1992. A comparison of two hormonal treatments on oestrous synchronization and embryo recovery in indigenous goats. Pre Conference Proceedings of the V International Conference on Goats, New Delhi, India, 2–8 March, 1992, vol. I, 374

  • Vadnere, S.V. and Mani, I., 1986. Superovulatory responses and embryo collection in goats. Proceedings of the17th Annual Meeting of Endocrine Society of India, Bombay, India, 4–8 November, 18–20

Download references

Author information

Authors and Affiliations

  1. Embryo Transfer Technology Laboratory, Physiology, Reproduction and Shelter Management Division, Central Institute for Research on Goats, Makhdoom, PO Farah-281122, Mathura, UP, India

    A. K. Goel & K. P. Agrawal

Authors
  1. A. K. Goel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. P. Agrawal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. K. Goel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goel, A.K., Agrawal, K.P. Ovulatory Response and Embryo Yield in Jakhrana Goats Following Treatments with PMSG and FSH. Trop Anim Health Prod 37, 549–558 (2005). https://doi.org/10.1007/s11250-005-4223-1

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-005-4223-1

Search

Navigation