Abstract
A rapid recombinant antigen-based latex agglutination test (LAT) has been developed to detect specific anti-leptospiral antibodies from human and dog sera. The recombinant LipL32 antigen developed and used for detecting the antibodies is specific in detection of the pathogenic serovars of Leptospira as the expression of the LipL32 antigen is restricted only to the pathogenic leptospires. The sensitized latex beads are stable and could be stored at 4°C for more than three months without showing loss of activity for both weakly and strongly positive samples. The test is found to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). Moreover, the recombinant antigen-coated latex beads could detect the specific anti-leptospiral antibodies in the acute phase of the illness. The test is simple and inexpensive, and is rapid in the management of large numbers of patients.
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Abbreviations
- LAT:
-
latex agglutination test
- MAT:
-
microscopic agglutination test
- ELISA:
-
enzyme-linked immunosorbent assay
References
Cole, J.R., Sulzer, C.R. and Pursell, A.R., 1973. Improved microtechnique for the leptospiral microscopic agglutination test. Applied Microbiology, 25, 976–980
Cousins, D.V., Robertson, G.M. and Hustas, L., 1985. The use of the enzyme linked immunosorbent assay (ELISA) to detect the IgM and IgG antibody response to Leptospira interrogans serovars hardjo, pomona and tarassovi in cattle. Veterinary Microbiology, 10, 439–450
Cumberland, P.C., Everard, C.O.R. and Levett, P.N., 1999. Assessment of the efficacy of the IgM enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis. American Journal of Tropical Medicine and Hygiene, 61, 731–734
Dey, S., Madhan Mohan, C., Senthil Kumar, T.M.A., Ramadass, P., Mahalinga Nainar, A., Nachimuthu, K., 2004. Recombinant LipL32 antigen based single serum dilution ELISA for detection of canine leptospirosis. Veterinary Microbiology, 103, 99–106
Haake, D.A., Chao, G., Zuerner, R.L., Barnett, J., Barnett, D., Mazel, M., Matsunaga, J., Levett, P.N., and Bolin, C.A., 2000. The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection. Infection and Immunity, 68, 2276–2285
Lengyel, A., Adam, E. and Nasz, I. 1993, Latex agglutination and adenoviruses. II. Detection of antigens. Acta Microbiology Hungarica, 40, 85–90
Levett, P.N., 2001. Leptospirosis. Clinical Microbiological Reviews, 14, 296–326
Muraschi, T.F., 1959. A simple screening test for the detection of leptospirosis in human beings and animals. American Journal of Public Health, 49, 1074–1078
Ramadass, P., Samuel, B. and Nachimuthu, K., 1999. A rapid latex agglutination test for the detection of leptospiral antibodies. Veterinary Microbiology, 70, 137–140
Ribotta, M.J., Higgins, M., Gottschalk, M. and Lallier, R., 2000. Development of an indirect enzyme linked immunosorbent assay for the detection of leptospiral antibodies in dogs. The Canadian Journal of Veterinary Research, 64, 32–37
Smits, H.L., Vander Hoorn, M.A.W.G., Goris, M.G.A., Gussenhoven, G.C., Yersin, C., Sasaki, D.M., Terpstra, W.J. and Hartskeerl, R.A., 2000. Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis. Journal of Clinical Microbiology 38, 1272–1275
Terpstra, W.J., Ligthart, G.S. and Schoone, G.J. 1985. ELISA for the detection of specific IgM and IgG in human leptospirosis. Journal of General Microbiology, 131, 377–385
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Dey, S., Madhan Mohan, C., Ramadass, P. et al. Recombinant Antigen-based Latex Agglutination Test for Rapid Serodiagnosis of Leptospirosis. Vet Res Commun 31, 9–15 (2007). https://doi.org/10.1007/s11259-006-3364-7
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DOI: https://doi.org/10.1007/s11259-006-3364-7