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Presence of rose spring dwarf-associated virus in Chile: partial genome sequence and detection in roses and their colonizing aphids

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Abstract

Rose is one of the most important cut flowers produced in the world. It is also grown in landscape plantings and public gardens for ornamental purposes. However, there is no detailed information available about viruses infecting roses in Chile. In order to gain insight about the viruses that could be present, a plant showing yellow vein chlorosis in its leaves was collected from a garden in Santiago. Double-stranded RNA (dsRNA) was isolated and after a random primed RT-PCR amplification procedure followed by sequencing, Rose spring dwarf-associated virus (RSDaV) presence was established. In order to widen the survey, several additional symptomatic and asymptomatic plants as well as aphids were screened by RT-PCR using two different pairs of virus-specific primers. RSDaV was detected in 24% of the analyzed samples. To our knowledge, this is the first report of RSDaV in Chilean rose plants and Rhodobium porosum (Sanderson) aphids.

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Acknowledgments

This study was partially supported by the CCTE/PFB-16 program from CONICYT (Chile) and Universidad Andrés Bello Young Scientist Award to EAE. Authors thank Dr. Pablo D.T. Valenzuela, Dr. Mario Rosemblatt, and Dr. Erwin Krauskopf for support and comments on this manuscript.

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Authors and Affiliations

  1. Fundación Ciencia para la Vida and Instituto Milenio de Biología Fundamental y Aplicada, Zañartu, 1482, Nunoa, Santiago, Chile

    Paulina A. Rivera & Esteban A. Engel

  2. Facultad de Ciencias Biológicas, Universidad Andrés Bello, República 252, Santiago, Chile

    Esteban A. Engel

Authors
  1. Paulina A. Rivera
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  2. Esteban A. Engel
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Correspondence to Esteban A. Engel.

Additional information

The nucleotide sequence data reported in this article have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers HM236354, HM236355, HM236356, HM236357, HM236358, HM236359, HM236360, HM236361, HM236362, HM236363, HM236364, HM236365, HM236366, and HM236367.

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Rivera, P.A., Engel, E.A. Presence of rose spring dwarf-associated virus in Chile: partial genome sequence and detection in roses and their colonizing aphids. Virus Genes 41, 295–297 (2010). https://doi.org/10.1007/s11262-010-0510-7

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  • DOI: https://doi.org/10.1007/s11262-010-0510-7

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