Abstract
We examined the effectiveness of rhizoaugmentation for treating water contaminated with the nitrophenols (NPs), 2-NP, 3-NP, 4-NP, and 2,4-dinitrophenol (2,4-DNP) using NP-degrading bacteria. We used 2-NP-degrading Pseudomonas sp. (strain ONR1), 3-NP-degrading Cupriavidus sp. (MFR2), 4-NP-degrading Rhodococcus sp. (PKR1), 2,4-DNP-degrading Rhodococcus sp. (DNR2), and giant duckweed (Spirodela polyrhiza). The four bacterial strains readily colonized Spirodela roots, as approximately 1 × 105 colony-forming units [CFUs] plant−1 to 106–107 CFU plant−1. The higher populations remained stable through five sequential 2-day degradation cycles and completely removed all four NPs within each cycle. The root–bacteria association also successfully treated wastewater effluent contaminated with NPs; 52–71 % of 2-NP and 100 % of 3-NP, 4-NP, and 2,4-DNP were removed within each of five 2-day cycles. These results demonstrate the potential of rhizoaugmentation to achieve efficient and sustainable treatment of NP-contaminated waters.
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Agency for Toxic Substances and Disease Registry (ATSDR) (1992). Toxicology profile for nitrophenols. http://www.atsdr.cdc.gov/toxprofiles/tp50.pdf. Accessed 10 Feb 2014.
Chaudhry, Q., Blom-Zandstra, M., Gupta, S., & Joner, E. J. (2005). Utilizing the synergy between plants and rhizosphere microorganisms to enhance breakdown of organic pollutants in the environment. Environmental Science and Pollution Research International, 12(1), 34–48.
Ghazali, F. M., Rahman, R. N. Z. A., Salleh, A. B., & Basri, M. (2004). Biodegradation of hydrocarbons in soil by microbial consortium. International Biodeterioration and Biodegradation, 54, 61–67.
Goux, S., Shapir, N., El Fantroussi, S., Lelong, S., Agathos, S. N., & Pussemier, L. (2003). Long-term maintenance of rapid atrazine degradation in soils inoculated with atrazine degraders. Water, Air, and Soil Pollution, 3, 131–142.
Hazardous Substances Data Bank (HSDB) (1999). Nitrophenols. http://toxnet.nlm.nih.gov/cgi-bin/sis/search/f?./temp/~M3C9oV:1. Accessed 10 Feb 2014.
Heinaru, E., Merimaa, M., Viggor, S., Lehiste, M., Leito, I., Truu, J., et al. (2005). Biodegradation efficiency of functionally important population selected for bioaugmentation in phenol- and oil-polluted area. FEMS Microbiological Ecology, 51(3), 363–373.
Hoang, H., Inoue, D., Momotani, N., Yu, N., Toyama, T., Sei, K., et al. (2009). Characterization of novel 4-n-butylphenol degrading Pseudomonas veronii strains isolated from rhizosphere of giant duckweed, Spirodela polyrhiza. Japanese Journal of Water Treatment Biology, 45, 83–92.
Japan Ministry of Environment (2010). The detailed environmental survey in fiscal year 2009. http://www.env.go.jp/chemi/kurohon/en/http2010e/pdf/04_Chapter2.pdf. Accessed 7 June 2011.
Kristanti, R. A., Kanbe, M., Toyama, T., Tanaka, Y., & Mori, K. (2012a). Accelerated biodegradation of nitrophenols in the rhizosphere of Spirodela polyrhiza. Journal of Environmental Science, 24(5), 800–807.
Kristanti, R. A., Kanbe, M., Hadibarata, T., Toyama, T., Tanaka, Y., & Mori, K. (2012b). Isolation and characterization of 3-nitrophenol degrading bacteria associated with rhizosphere of Spirodela polyrhiza. Environmental Science and Pollution Research, 19, 1852–1858.
Kuiper, I., Lagendijk, E. L., Bloemberg, G. V., & Lugtenberg, B. J. (2004). Rhizoremediation: a beneficial plant-microbe interaction. Molecular Plant-Microbe Interactions, 17(1), 6–15.
Mori, K., Toyama, T., & Sei, K. (2005). Surfactant degrading activities in the rhizosphere of giant duckweed (Spirodela polyrhiza). Japanese Journal of Water Treatment Biology, 41, 129–140.
Mrozik, A., & Piotrowska-Seget, Z. (2010). Bioaugmentation as a strategy for cleaning up of soils contaminated with aromatic compounds. Microbiological Research, 165(5), 363–375.
Ogata, Y., Momotani, N., Toyama, T., Inoue, D., Sei, K., Soda, S., et al. (2009). Occurence of 4-n-butylphenol degradation in aquatic samples caused by the presence of Spirodela polyrhiza. Biodegradation, 24, 191–202.
Olson, P. E., Castro, A., Joern, M., DuTeau, N. M., Pilon-Smits, E. A. H., & Reardon, K. F. (2007). Comparison of plant families in a greenhouse phytoremediation study on an aged polycyclic aromatic hydrocarbon-contaminated soil. Journal of Environmental Quality, 36, 1461–1469.
Phillips, L. A., Greer, C. W., & Germida, J. J. (2006). Culture-based and culture-independent assessment of the impact of the mixed and single plant treatments on rhizosphere microbial communities in hydrocarbon contaminated flare-pit soil. Soil Biology and Biochemistry, 38, 2823–2833.
Ryslava, E., Krejcik, Z., Macek, T., Novakova, H., Demnerova, K., & Mackova, M. (2003). Study of PCB degradation in real contaminated soil. Fresenius Environmental Bulletin, 12, 296–301.
Siciliano, S. D., Germida, J. J., Banks, K., & Greer, C. W. (2003). Changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial. Applied Environmental Microbiology, 69, 483–489.
Spain, J. S. (1995). Biodegradation of nitro-aromatic compounds. Annual Reviews of Microbiology, 49, 523–555.
Toyama, T., Yu, N., Kumada, H., Sei, K., Ike, M., & Fujita, M. (2006). Accelerated aromatic compounds degradation in aquatic environment by use of interaction between Spirodela polyrhiza and bacteria in its rhizosphere. Journal of Bioscience and Bioengineering, 101, 346–353.
US Environmental Protection Agency (2011). Priority pollutant. http://water.epa.gov/scitech/methods/cwa/pollutants.cfm. Accessed 7 June 2011.
Wood, T. K. (2008). Molecular approaches in bioremediation. Current Opinion in Biotechnology, 19(6), 572–578.
World Health Organization (2000). Concise International Chemical Assessment Document 20, Mononitrophenols. http://www.who.int/ipcs/publications/cicad/en/cicad20.pdf. Accessed 7 June 2011.
Acknowledgments
This research was supported by the Japan Science and Technology Agency (JST) as part of the Advanced Low Carbon Technology Research and Development Program (ALCA) with the research theme of “Development of highly-ordered vegetational bioprocesses utilizing symbiotic interactions in the rhizosphere.”
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Kristanti, R.A., Toyama, T., Hadibarata, T. et al. Sustainable Removal of Nitrophenols by Rhizoremediation Using Four Strains of Bacteria and Giant Duckweed (Spirodela polyrhiza). Water Air Soil Pollut 225, 1928 (2014). https://doi.org/10.1007/s11270-014-1928-7
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DOI: https://doi.org/10.1007/s11270-014-1928-7