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Study of Arsenic-Contaminated Soil Bacterial Community Using Biochip Technology

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Abstract

The ecosystems near arsenic mining industrial areas are characterized with an elevated level of pollutants. In Caucasus region, such a hotspot is presented in Western Georgia: Uravi and Tsana abandoned arsenic production facilities and nearby mining tailings stored in deteriorating conditions that pose a threat to the population. The research presents a study of the local bacteria community of highly arsenic-contaminated soils (from 400 mg/kg at Uravi arsenic sulfide mineral processing facility to 11.3 g/kg at arsenic oxide storage area in Tsana) using an innovative, multitasking microscale bioanalytical method for environmental enquiries – DNA biochip (microarray). The detected Shewanella spp., Bacillus spp., and sulfate-reducing bacteria were considered as promising objects for future projects on in situ recovery of vast arsenic-contaminated areas applying remediation methods.

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Acknowledgments

We would like to acknowledge warmly our colleagues for the support and assistance: Professor Tengiz Urushadze, Head of Michail Sabashvili Institute of Soil Science, Agrochemistry and Melioration, Agricultural University of Georgia, for the guidance in the sphere of soil sciences and characterization of the sampling sites; Professor Guram Gioshvili, Professor George Adamia, and MSc Mirian Makadze for the assistance in the framework of the project.

Funding

This work was supported by grants (#2016–39) from Shota Rustaveli National Science Foundation (SRNSF) and (#6304) from the Science and Technology Center in Ukraine (STCU).

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Authors and Affiliations

  1. Durmishidze Institute of Biochemistry and Biotechnology, Agricultural University of Georgia, Kakha Bendukidze University Campus, 240 David Aghmashenebeli Alley, 0159, Tbilisi, Georgia

    Evgeni Bunin, Gia Khatisashvili & Tamar Varazi

  2. Andronikashvili Institute of Physics, I. Javakhishvili Tbilisi State University, 6 Tamarashvili Str, 0162, Tbilisi, Georgia

    Tamar Kartvelishvili, Nino Asatiani & Nelly Sapojnikova

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  1. Evgeni Bunin
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Correspondence to Nelly Sapojnikova.

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Bunin, E., Khatisashvili, G., Varazi, T. et al. Study of Arsenic-Contaminated Soil Bacterial Community Using Biochip Technology. Water Air Soil Pollut 231, 198 (2020). https://doi.org/10.1007/s11270-020-04575-1

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