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Characterization and purification of a bacteriocin from Lactobacillus paracasei subsp. paracasei BMK2005, an intestinal isolate active against multidrug-resistant pathogens

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Abstract

A strain of Lactobacillus paracasei subsp. paracasei BMK2005 isolated from healthy infant faeces has shown a remarkable antibacterial activity against 32 bacterial pathogenic strains of human clinical isolates. Among them, 13 strains belonging to species of Escherichia coli, Citrobacter freundii, Citrobacter diversus, Klebsiella oxytoca, Enterobacter cloacae and Pseudomonas aeruginosa were resistant to Cefotaxime (CTX) and Ceftazidime (CAZ), and 4 strains of Staphylococcus aureus were resistant to Methicillin (MRSA). This antibacterial activity was attributed to a bacteriocin designated as Paracaseicin A. It was heat-stable up to 120°C for 5 min and active within the pH range of 2–5. Its activity was lost when treated with proteases, which reveals its proteinaceous nature. This bacteriocin was successfully purified only by two steps of reversed phase chromatography. Its molecular mass, determined by mass spectrometry analysis, was 2,462.5 Da. To our knowledge, the present study is the first report on characterization and purification of a bacteriocin, produced by a L. paracasei subsp. paracasei strain exhibiting an antibacterial activity against various multidrug-resistant species of Gram-positive and Gram-negative bacteria, which reveals its potential for use in prevention or treatment of infections caused by multidrug-resistant species especially in cases of antibiotics-associated diarrhea (AAD).

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Acknowledgments

This study has been accomplished thanks to a research project financed by the Algerian Ministry of Higher Education and Scientific Research, and the Laboratory of ISM2, Faculty of Saint–Jerome, University of Aix-Marseille III, France. The authors would like to thank Dr. Marius Reglier, Dr. Emmanuelle Crost, Dr. André Moulin, Dr. Adjendouz El-Hassen and Dr. Harivoni Rakotoarivonina, from the laboratory of ISM2 for their collaboration and for helping to perform the experiments with HPLC and DNA analysis. As well, we are grateful to Dr. Claude Villard, Faculty of Pharmacy, La Timone, France, for the MALDI-TOF mass spectrometry analysis.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire de Microbiologie Appliquée (LMA), Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université de Béjaia, Route de Targa Ouzemmour, Béjaia, Algeria

    Kamel Bendjeddou & Djamila Sadoun

  2. Laboratoire ISM2/Biosciences UMR CNRS 6263, Université Paul Cézanne Aix-Marseille III, Faculté des Sciences et Techniques de St Jérôme, Service 342, 13397, Marseille Cedex 20, France

    Michel Fons & Pierre Strocker

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  1. Kamel Bendjeddou
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  4. Djamila Sadoun
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Correspondence to Kamel Bendjeddou.

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Bendjeddou, K., Fons, M., Strocker, P. et al. Characterization and purification of a bacteriocin from Lactobacillus paracasei subsp. paracasei BMK2005, an intestinal isolate active against multidrug-resistant pathogens. World J Microbiol Biotechnol 28, 1543–1552 (2012). https://doi.org/10.1007/s11274-011-0958-1

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  • DOI: https://doi.org/10.1007/s11274-011-0958-1

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