Abstract
Electron microscopic observation revealed that lupeol induced melanosome maturation in B16 2F2 mouse melanoma cells and we therefore studied the effects of lupeol on the intracellular events responsible for melanosome transport. Incubation with lupeol for 8 h attenuated the actin stress fiber assembly in B16 2F2 mouse melanoma cells, resulting in dendritic formation in the cells. Longer exposure to lupeol (48 h) increased the expression of tyrosinase, MITF (a specific transcription factor for tyrosinase), Rab27a, and myosin-Va, which are required for melanosome transport.
Similar content being viewed by others
Abbreviations
- MITF:
-
Microphthalmia-associated transcriptional factor
- TEM:
-
Transmission electron microscopy
References
Virador VM, Muller J, Wu X, Abdel-Malek ZA, Yu ZX, Ferrans VJ, Kobayashi N, Wakamatsu K, Ito S, Hammer JA, Hearing VJ (2002) Influence of alpha-melanocyte-stimulating hormone and ultraviolet radiation on the transfer of melanosomes to keratinocytes. FASEB J 106:105–107
Vancoillie G, Lambert J, Mulder A, Koerten HK, Mommaas AM, Van Oostveldt P, Naeyaert JM (2000) Kinesin and kinectin can associate with the melanosomal surface and form a link with microtubules in normal human melanocytes. J Invest Dermatol 114:421–429
Byers HR, Yaar M, Eller MS, Jalbert NL, Gilchrest BA (2000) Role of cytoplasmic dynein in melanosome transport in human melanocytes. J Invest Dermatol 114:990–997
Fukuda M, Kuroda TS, Mikoshiba K (2002) Salc2-a/Melanophilin, the missing link between Rab27 and myosin Va. J Biol Chem 277:12432–12436
Langford GM (2002) Myosin V, a versatile mortar for short-range vehicle transport. Traffic 3:859–865
Busca R, Bertolotto C, Ortonne JP, Ballotti R (1995) Inhibition of the phosphatidylinositol 3-kinase/p70S6-kinase pathway induces B16 melanoma cell differentiation. J Biol Chem 271:31824–31830
Busca R, Bertolotto C, Abbe P, Engalo W, Ishizaki T, Narumiya S, Boquet P, Ortonne JP, Balotti R (1998) Inhibition of Rho is required for cAMP-induced melanoma cell differentiation. Mol Cell Biol 9:1367–1378
Passeron T, Bahadoran P, Bertolotto C, Chiaverini C, Buscà R, Valony G, Bille K, Ortonne JP, Ballotti R (2004) Cyclic AMP promotes a peripheral distribution of melanosomes and stimulates melanophilin/Slac2-a and actin association. FASEB J 18:989–991
Hata K, Mukaiyama T, Tsujimura N, Sato Y, Kosaka Y, Sakamoto K, Hori K (2006) Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line. Cytotechnology 52:151–158
Scott G, Leopardi S (2003) The cAMP signaling pathway has opposing effects on Rac and Rho in B16F10cells: implications for dendrite formation in melanocyte cells. Pigment Cell Res 16:139–148
Hata K, Hori K, Murata J, Takahashi S (2005) Remodeling of actin cytoskeleton in lupeol-induced B16 2F2 cell differentiation. J Biochem 138:467–472
Acknowledgments
This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ogiwara, K., Hata, K. Melanoma cell differentiation induced by lupeol separates into two stages: morphological and functional changes. J Nat Med 63, 323–326 (2009). https://doi.org/10.1007/s11418-009-0319-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11418-009-0319-7