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Evaluation of anti-inflammatory and antinociceptive effects of D-002 (beeswax alcohols)

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Abstract

D-002, a mixture of six higher aliphatic alcohols purified from beeswax, displayed anti-inflammatory effects in carrageenan-induced pleurisy and cotton pellet granuloma in rats. The aim of the present study was to confirm the anti-inflammatory properties of D-002 and to explore its potential analgesic effects. Xylene-induced mouse ear oedema was used to assess the anti-inflammatory effect, acetic acid-induced writhing and hot plate responses for the analgesic activity, and the open field and horizontal rotarod tests for motor performance. For anti-inflammatory tests, mice were randomised into a negative vehicle control and five xylene-treated groups: the vehicle, D-002 (25, 50 and 200 mg/kg) and indomethacin 1 mg/kg (reference drug). Treatments were given for 15 days. Effects on oedema formation and myeloperoxidase (MPO) activity were tested. For analgesia and motor performance tests, mice were randomised into a vehicle control and D-002-treated groups (25, 50 and 200 mg/kg). Two sets of experiments were done, which included acute and repeat (15 days) dosing. D-002 (25, 50 and 200 mg/kg) significantly decreased xylene-induced ear oedema (44.7, 60.8 and 76.4%, respectively) and the increase of MPO activity induced by xylene (38.0, 47.0 and 57.0%, respectively), while indomethacin significantly inhibited xylene-induced oedema (59.9%) and MPO activity (57.5%). Single and repeat doses of D-002 (25, 50 and 200 mg/kg) decreased the acetic acid-induced writhing responses by 21.2, 28.2 and 40.1%, for the single doses; 25.2, 35.1 and 43.2%, respectively, for the repeat doses, but did not affect the hot plate, open field and rotarod behaviours. Aspirin 100 mg/kg significantly decreased acetic acid-induced abdominal constrictions and morphine (5 mg/kg) significantly increased the latency of the hot plate response. This study confirmed the anti-inflammatory effects of D-002 and demonstrated its analgesic effects on the acetic acid-induced writhing, but not on the hot plate response, which suggests that the antinociceptive effects of D-002 could be related to its anti-inflammatory activity.

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Authors and Affiliations

  1. Pharmacology Department, Centre of Natural Products, National Centre for Scientific Research, 198 Ave Between 19 and 21 sts., Atabey, Playa, Havana, Cuba

    Yazmin Ravelo, Vivian Molina, Daisy Carbajal, Lilia Fernández, Julio C. Fernández, María L. Arruzazabala & Rosa Más

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  1. Yazmin Ravelo
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  2. Vivian Molina
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  3. Daisy Carbajal
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  4. Lilia Fernández
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  5. Julio C. Fernández
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  6. María L. Arruzazabala
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  7. Rosa Más
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Correspondence to Daisy Carbajal.

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Ravelo, Y., Molina, V., Carbajal, D. et al. Evaluation of anti-inflammatory and antinociceptive effects of D-002 (beeswax alcohols). J Nat Med 65, 330–335 (2011). https://doi.org/10.1007/s11418-010-0496-4

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  • DOI: https://doi.org/10.1007/s11418-010-0496-4

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