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Treatment of chronical myocardial ischemia by adenovirus-mediated hepatocyte growth factor gene transfer in minipigs

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Abstract

Growth factor gene transfer-induced therapeutic angiogenesis has become a novel approach for the treatment of myocardial ischemia. In order to provide a basis for the clinical application of an adenovirus with hepatocyte growth factor gene (Ad-HGF) in the treatment of myocardial ischemia, we established a minipig model of chronically ischemic myocardium in which an Ameroid constrictor was placed around the left circumflex branch of the coronary artery (LCX). A total of 18 minipigs were randomly divided into 3 groups: a surgery control group, a model group and an Ad-HGF treatment group implanted with Ameroid constrictor. Ad-HGF or the control agent was injected directly into the ischemic myocardium, and an improvement in heart function and blood supply were evaluated. The results showed that myocardial perfusion remarkably improved in the Ad-HGF group compared with that in both the control and model groups. Four weeks after the treatment, the density of newly formed blood vessels was higher and the number of collateral blood vessels was greater in the Ad-HGF group than in the model group. The area of myocardial ischemia reduced evidently and the left ventricular ejection fraction improved significantly in the Ad-HGF group. These results suggest that HGF gene therapy may become a novel approach in the treatment of chronically ischemic myocardium.

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Authors and Affiliations

  1. The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

    Biao Yuan, Zhong Zhao & Jun Huang

  2. Beijing Institute of Radiation Medicine, Beijing, 100850, China

    YouRong Zhang, DanLi Wu, LiZhen Yuan, Bin Wu & LiSheng Wang

Authors
  1. Biao Yuan
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  2. YouRong Zhang
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  3. Zhong Zhao
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  4. DanLi Wu
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  5. LiZhen Yuan
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  6. Bin Wu
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  7. LiSheng Wang
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  8. Jun Huang
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Corresponding author

Correspondence to Jun Huang.

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Contributed equally to this work

Supported by National “863” Programme (Grant Nos. 2003AA216080 and 2007AA021007)

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Yuan, B., Zhang, Y., Zhao, Z. et al. Treatment of chronical myocardial ischemia by adenovirus-mediated hepatocyte growth factor gene transfer in minipigs. SCI CHINA SER C 51, 537–543 (2008). https://doi.org/10.1007/s11427-008-0073-1

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  • DOI: https://doi.org/10.1007/s11427-008-0073-1

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