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Glutathione-Protected Hierarchical Colorimetric Response of Gold Nanoparticles: a Simple Assay for Creatinine Rapid Detection by Resonance Light Scattering Technique

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Abstract

Although numerous methods have been reported for the analysis of creatinine in human serum, the development of a simple, rapid, and practical sensor still remains a great challenge. In this work, a hierarchical colorimetric sensor was demonstrated based on the anti-aggregation effect of glutathione (GSH)-protected gold nanoparticles (AuNPs). Creatinine molecule could induce the aggregation of AuNPs, and the system could show blue color simultaneously. When GSH was added to incubate with AuNPs in advance, the solution could perform a hierarchical color change corresponding to different concentration of creatinine. Consequently, the change of AuNPs in size resulted in the difference of resonance light scattering (RLS) intensity and the quantitative detection of creatinine could be achieved. Meanwhile, the determination of creatinine in human serum could be attained with a detection limit of 1.21 μM, and the colorimetric sensor could be applied to detect creatinine in human serum successfully in a wide range from 10 to 1000 μM. As above, the creatinine in human serum could be distinguished using proper concentration of GSH. More practically, we could identify if the sample exceeded or below the critical value with our naked eye. This sensing proposal was accompanied with prominent simplicity, speediness, and practicability clinically.

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Acknowledgments

We are grateful for the financial support from the Research Start-up Funding of Shantou University (No. NTF10002), the Natural Science Foundation of Guangdong Province (No. S2011010005208), the Guangdong High Education Fund of Science and Technology Innovation (No. 2013KJCX0078), and the National Natural Science Foundation of China (No.51272152/E0208).

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    This manuscript has not been submitted to more than one journal for simultaneous consideration.

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    The manuscript has not been published previously (partly or in full).

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    A single study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time.

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    No data have been fabricated or manipulated (including images) to support the conclusions.

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    No data, text, or theories by others are presented as if they were the authors own.

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    Consent to submit has been received from all coauthors and responsible authorities.

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Authors and Affiliations

  1. Department of Chemistry, Shantou University, Shantou, Guangdong, 515063, China

    Xiaopeng Huang, Yingjie Li, Jiahong Pan, Fushen Lu & Wenhua Gao

  2. Analysis & Testing Center, Shantou University, Shantou, Guangdong, 515063, China

    Yaowen Chen & Wenhua Gao

Authors
  1. Xiaopeng Huang
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  2. Yingjie Li
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  3. Jiahong Pan
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  4. Fushen Lu
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  5. Yaowen Chen
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  6. Wenhua Gao
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Corresponding author

Correspondence to Wenhua Gao.

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Huang, X., Li, Y., Pan, J. et al. Glutathione-Protected Hierarchical Colorimetric Response of Gold Nanoparticles: a Simple Assay for Creatinine Rapid Detection by Resonance Light Scattering Technique. Plasmonics 10, 1107–1114 (2015). https://doi.org/10.1007/s11468-015-9907-4

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