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Protective Effect of Salidroside on Mitochondrial Disturbances via Reducing Mitophagy and Preserving Mitochondrial Morphology in OGD-induced Neuronal Injury

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Summary

Salidroside is the active ingredient extracted from Rhodiola rosea, and has been reported to show protective effects in cerebral ischemia, but the exact mechanisms of neuronal protective effects are still unrevealed. In this study, the protective effects of salidroside (1 µmol/L) in ameliorating neuronal injuries induced by oxygen-glucose deprivation (OGD), which is a classical model of cerebral ischemia, were clarified. The results showed that after 8 h of OGD, the mouse hippocampal neuronal cell line HT22 cells showed increased cell death, accompanied with mitochondrial fragmentation and augmented mitophagy. However, the cell viability of HT22 cells showed significant restoration after salidroside treatment. Mitochondrial morphology and mitochondrial function were effectively preserved by salidroside treatment. The protective effects of salidroside were further related to the prevention of mitochondrial over-fission. The results showed that mTOR could be recruited to the mitochondria after salidroside treatment, which might be responsible for inhibiting excessive mitophagy caused by OGD. Thus, salidroside was shown to play a protective role in reducing neuronal death under OGD by safeguarding mitochondrial function, which may provide evidence for further translational studies of salidroside in ischemic diseases.

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Author notes

  1. These authors contribute equally to the work.

Authors and Affiliations

  1. Department of Cardiology, Wuhan Red Cross Hospital, Wuhan, 430015, China

    Cai-ying Hu

  2. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Qian-ying Zhang, Jie-hui Chen, Bin Wen, Wei-jian Hang, Kai Xu & Juan Chen

  3. Department of Clinical Laboratory, Affiliated Wuhan Mental Health Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Qian-ying Zhang

  4. Division of Cardiology, Department of Internal Medicine, and Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Wei-jian Hang

  5. Department of Cardiovascular Medicine, Lichuan People’s Hospital, Lichuan, 445400, China

    Ben-hong He

Authors
  1. Cai-ying Hu
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  2. Qian-ying Zhang
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  7. Juan Chen
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  8. Ben-hong He
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Corresponding authors

Correspondence to Juan Chen or Ben-hong He.

Additional information

This work was supported by grants from the National Natural Science Foundation of China (Nos. 81873725, 81371416, and 31670778) and Hubei Province’s Outstanding Medical Academic Leader Program.

Conflict of Interest Statement

The authors declare no conflict of interest regarding the work.

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Hu, Cy., Zhang, Qy., Chen, Jh. et al. Protective Effect of Salidroside on Mitochondrial Disturbances via Reducing Mitophagy and Preserving Mitochondrial Morphology in OGD-induced Neuronal Injury. CURR MED SCI 41, 936–943 (2021). https://doi.org/10.1007/s11596-021-2374-6

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  • DOI: https://doi.org/10.1007/s11596-021-2374-6

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