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Micropropagation and in vitro production of secondary metabolites of Croton floribundus Spreng.

  • Molecular Farming/Metabolic Engineering/Secondary Metabolism
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Abstract

Croton floribundus Spreng., a native plant from South America, was utilized for in vitro micropropagation and phytochemical analyses. The effects of the addition of naphthaleneacetic acid and indole butyric acid, on the production of shoots and leaves, as well as volatile constituent production, were determined. The combination of naphthaleneacetic acid and indole butyric acid at a ratio of 1:1 led to the production of the maximum number of leaves and longest shoots after a 60-d subculture period. Analyses of leaf dichloromethane extracts using gas chromatography–mass spectrometry showed that monoterpenes and sesquiterpenes were the main chemical classes present in both in vivo and in vitro conditions. Use of these plant growth regulators in the medium-induced quantitative changes in the major monoterpenes (neral, geranial, limonene, and carvone). In vitro leaf extracts produced compounds such as carvone as well as a large amount of trans-β-farnesene, with the highest production of carvone (16.8%) being produced on medium supplemented with 1.0 mg L−1 naphthaleneacetic acid. These results suggested the occurrence of biotransformation reactions of limonene and farnesyl cations in culture.

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References

  • Adams RP (2001) Identification of essential oils components by gas chromatography/quadrupole mass spectroscopy. Carol Sream IL: 1st Allured Publishing Corporation, Carol Stream IL, USA

  • Avato P, Fortunato IM, Ruta C, D’Elia R (2005) Glandular hairs and essential oils in micropropagated plants of Salvia officinalis L. Plant Sci 169:29–36

    Article  CAS  Google Scholar 

  • Berry PE, Hipp AL, Wurdack KJ, Van EBW, Riina R (2005) Molecular phylogenetics of the giant genus Croton and tribe Crotoneae (Euphorbiaceae sensu stricto) using ITS and trnL-trnF sequence data. Am J Bot 92:1520–1534

    Article  PubMed  CAS  Google Scholar 

  • Block S, Baccelli C, Tinant B, Meervelt LC, Rozenberg R, Jiwan JLH (2004) Llabrès, G.; Pauw-Gillet, M.C.; Quetin-Leclercq, J. Diterpenes from the leaves of Croton zambesicus. Phytochem 65:1165–1171

    Article  CAS  Google Scholar 

  • Brasil DSB, Muller AH, Guilhon GMSP, Alves CN, Andrade EHA, Silva JKR, Maia JGS (2009) Essential oil composition of Croton palanostigma Klotzsch from north Brazil. J Braz Chem Soc 20(6):1188–1192

    Article  CAS  Google Scholar 

  • Burger W, Huft M (1995) Family 113 Euphorbiaceae. Flora Costaricensis. Fieldiana, Bot New Series 36

  • Carvalho PER (2003) Espécies arbóreas brasileiras. Brasília: Embrapa Inf Tecnol 1:335–341

    Google Scholar 

  • Castro SBR, Leal CAG, Freire FR, Carvalho DA, Oliveira DF Figueiredo HCP (2008) Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria. Braz J Microbiol 39:756–760

    Article  Google Scholar 

  • Chan Y, Walmsley RP (1997) Learning and understanding the Kruskal–Wallis one-way analysis-of-variance-by ranks test for differences among three or more independent groups. Phys Ther 77:1755–1762

    PubMed  CAS  Google Scholar 

  • Craveiro AA, Andrade CHS, Matos FJA, Alencar JW (1978) Anise-like flavour of Croton aff zehntneri Pax. Et Hoffm. J Agric Food Chem 26:772–773

    Article  CAS  Google Scholar 

  • De Carvalho C, Da Fonseca MMR (2003) Towards the bio-production of trans-carveol and carvone from limonene: induction after cell growth on limonene and toluene. Tetrahedron-Asymmetry 14:3925–3931

    Article  Google Scholar 

  • De-Eknamkul W, Potduang B (2003) Biosynthesis of β-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units. Phytochem 62:389–398

    Article  CAS  Google Scholar 

  • Dörnenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzym Microb Technol 17:674–684

    Article  Google Scholar 

  • Duetz WA, Bouwmeester H, Beilen JB, Witholt B (2003) Biotransformation of limonene by bacteria, fungi, yeasts and plants. Appl Microbiol Biotechnol 61:269–277

    PubMed  CAS  Google Scholar 

  • Dunn OJ (1961) Multiple comparisons among means. J Am Stat Assoc. 56:52–64

    Article  Google Scholar 

  • Govaerts R, Frodin DG, Radcliffe-Smith A (2000) World Checklist and bibliography of Euphorbiaceae (and Pandaceae), 4 parts. Royal Botanic Gardens, Kew

    Google Scholar 

  • Grzegorczyk I, Matkowski A, Wysokinska H (2007) Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem 104:536–541

    Article  CAS  Google Scholar 

  • Jones K (2003) Review of Sangre de Drago (Croton lechleri). A South American tree sap in the treatment of diarrhea, inflammation, insect bites, viral infections, and wounds: traditional uses to clinical research. J Altern Complement Med 9(6):877–896

    Article  PubMed  Google Scholar 

  • Kruskal WH, Wallis WA (1952) Use of ranks in one-criterion variance analysis. J Amer Stat Ass 47: 583–621

    Article  Google Scholar 

  • Lange BM, Wildung MR, Stauber EJ Sanchez C, Pouchnik D, Croteau R (2000) Probing essential oil biosynthesis and secretion by functional evaluation of expressed sequence tags from mint glandular trichomes. Proc Natl Acad Sci USA 97:2934–2939

    Article  PubMed  CAS  Google Scholar 

  • Lima EC, Paiva R, Nogueira RC, Soares FP, Emrich EB, Silva AAN (2008) Callus induction in leaf segments of Croton urucurana Baill. Cienc Agrotec 32:17–22

    Article  CAS  Google Scholar 

  • Lindmark-Henriksson M, Isaksson D, Vaněk T, Valterová I, Högberg HE, Sjödin K (2004) Transformation of terpenes using a Picea abies suspension culture. J biotechnol 107:173–184

  • Luczkiewicz M, Glod D (2003) Callus cultures of Genista plants: in vitro material producing high amounts of isoflavones of phytoestrogenic activity. Plant Sci 165:1101–1108

    Article  CAS  Google Scholar 

  • Luczkiewicz M, Glod D (2005) Morphogenesis-dependent accumulation of phytoestrogens in Genista tinctoria in vitro cultures. Plant Sci 168:967–979

    Article  CAS  Google Scholar 

  • Marasco EK, Schmidt-Dannert C (2007) Biosynthesis of plant natural products and characterization of plant biosynthetic pathways in recombinant microorganisms. In: Verpoorte R, Alfermann AW, Johnson TS (eds) Applications of plant metabolic engineering, Springer, Dordrecht, Netherland, pp 1–43

  • McCaskill D, Croteau R (1997) In: Berger RG (ed) Biotechnology of aroma compounds. Advances in biochemical engineering biotechnology. Springer, Berlin

    Google Scholar 

  • Murashige T, Skoog FA (1962) revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ofusori DA, Oluwayinka OP, Adelakun AE, Keji ST, Oluyemi KA, Adesanya OA, Jeigbe KO, Ayoka AO (2007) Evaluation of the effect of ethanolic extract of Croton zambesicus on the testes of Swiss albino mice. Afr J Biotechnol 6:2434–2438

    Google Scholar 

  • Pinho-da-Silva L, Mendes-Maia PV, do Nascimento GTM, Cruz JS, de Morais SM, Coelho-de-Souza AN, Ralhou S, Leal-Cardoso JH (2010) Croton sonderianus essential oil samples distinctly affect rat airway smooth muscle. Phytomedicine 17:721–725

    Article  PubMed  CAS  Google Scholar 

  • Radulovic N, Mananjaraso E, Harinantenaina L, Yoshinori A (2006) Essential oil composition of four Croton species from Madagascar and their chemotaxonomy. Bioch Syst Ecol 34:648–653

    Article  CAS  Google Scholar 

  • Rao RS, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153

    PubMed  CAS  Google Scholar 

  • Rubin S, Lima CSM, Bandeira JM, Ribeiro MV, Benitz LC, Peters JA, Braga EJB (2007) Reguladores de crescimento na multiplicação in vitro de Thymus vulgaris L. Rev Bras Biocienc 5:480–482

    Google Scholar 

  • Salatino A, Salatino MLF, Negri G (2007) Traditional uses, chemistry and pharmacology of Croton species (Euphorbiaceae). J Braz Chem Soc 18:11–33

    Article  CAS  Google Scholar 

  • Santos PAG, Figueiredo AC, Oliveira MM, Barroso JG, Pedro LG, Deans SG, Scheffer JJC (2005) Growth and essential oil composition of hairy root cultures of Levisticum officinale W.D.J. Koch (lovage). Plant Sci 168:1089–1096

    Article  CAS  Google Scholar 

  • Santos-Gomes PC, Seabra RM, Fernandes-Ferreira M (2002) Phenolic antioxidant compounds produced by in vitro shoots of sage (Salvia officinalis L) Plant Sci 162:981–987

    Article  CAS  Google Scholar 

  • Secco RS (2004) Croton dissectistipulatus, a new species of Euphorbiaceae from Amazonian Brazil. Brittonia 56:353–356

    Article  Google Scholar 

  • Secco RS, Cordeiro I (2002) Notes on the first record of Croton urucurana Baill. (Euphorbiaceae) in Amazonia. Bol Mus Emilio Goeldi, Bot 18:195–201

    Google Scholar 

  • Shibata W, Murai F, Akiyama T, Siriphol M, Matsunaga E, Morimoto H (1996) Micropropagation of Croton sublyratus Kurge. a tropical tree of medicinal importance. Plant Cell Rep 16:147–152

    CAS  Google Scholar 

  • Shimizu Y, Imayoshi Y, Kato M, Maeda K, Iwabuchi H, Shimomura K (2009) Volatiles from leaves of field-grown plants and shoot cultures of Gynura bicolor DC. Flavour Fragr J 24:251–258

    Article  CAS  Google Scholar 

  • Silva S, Sato A, Lage CLS, San Gil RAS, Azevedo DA, Esquibel MA (2005) Essential oil composition of Melissa officinalis L. in vitro produced under the influence of growth regulators. J Braz Chem Soc 16:387–1390

    Google Scholar 

  • Simões C, Mattos JCP, Sabino KCC, Caldeira de Araújo A, Coelho MGP, Albarello N, Figueiredo SFL (2006) Medicinal potencial from in vivo and acclimatized plants of Cleome rosea Vahl ex DC. (Capparaceae). Fitoterapia 77:94–99

    Article  PubMed  Google Scholar 

  • Stockigt J, Obitz P, Falkenhagen H, Lutterbach R, Endreb S (1995) Natural products and enzymes from plant cell cultures. Plant Cell Tissue Organ Cult 43:97–109

    Article  Google Scholar 

  • Sudriá C, Pinol MT, Palazon J, Cusido RM, Vila R, Morales C, Bonfill M, Canigueral S (1999) Influence of plant growth regulators on the growth and essential oil content of cultured Lavandula dentata plantlets. Plant Cell Tiss Organ Cult 58:177–184

    Article  Google Scholar 

  • Sylvestre M, Pichette A, Longtin A, Nagaub F, Legault J (2006) Essential oil analysis and anticancer activity of leaf essential oil of Croton flavens L. from Guadeloupe. J Ethnopharmacol 103:99–102

    Article  PubMed  CAS  Google Scholar 

  • Vanek T, Valterová I, Vankov R, Vaisar T (1999) Biotransformation of (−)-limonene using Solanum aviculare and Dioscorea deltoidea immobilized plant cells. Biotechnol Lett 21:625–628

    Article  CAS  Google Scholar 

  • Wagner H, Bladt S (1995) Plant drug analysis, 2nd ed. Springer, Berlin

    Google Scholar 

  • Wungsintaweekul J, Sriyapai C, Kaewkerd S, Tewtrakul S, Kongduang D, De-Eknamkul W (2007) Establishment of Croton stellatopilosus suspension culture for geranylgeraniol production and diterpenoid biosynthesis. Z Naturforsch C 62:389–396

    PubMed  CAS  Google Scholar 

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Acknowledgments

The first author is grateful to Capes (Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro) for providing her with a doctoral fellowship.

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Authors and Affiliations

  1. Centro de Ciências da Saúde, Instituto de Biologia, Bl. A, Dep. Botânica, Laboratório de Ultraestrutura Vegetal, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Ilha do Fundão, Rio de Janeiro, RJ, Brazil, CEP: 21941-902

    Bianca Ortiz da Silva & Ricardo P. Louro

  2. Laboratório de Plantas Medicinais e Derivados, Departamento de Produtos Naturais, Farmanguinhos, Fiocruz, Rio de Janeiro, RJ, 21041-250, Brazil

    Ana Claudia F. Amaral & José Luiz P. Ferreira

  3. Laboratório de Biodiversidade e Biotecnologia—Departamento de Botânica, Instituto de Biociências, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Laura Jane Moreira Santiago

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  1. Bianca Ortiz da Silva
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  2. Ana Claudia F. Amaral
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  3. José Luiz P. Ferreira
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  4. Laura Jane Moreira Santiago
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  5. Ricardo P. Louro
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Correspondence to Ricardo P. Louro.

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Editor: Rida A. Shibli

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da Silva, B.O., Amaral, A.C.F., Ferreira, J.L.P. et al. Micropropagation and in vitro production of secondary metabolites of Croton floribundus Spreng.. In Vitro Cell.Dev.Biol.-Plant 49, 366–372 (2013). https://doi.org/10.1007/s11627-013-9494-z

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  • DOI: https://doi.org/10.1007/s11627-013-9494-z

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