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Incorporation of Alpha-Linolenic Acid and Enhancement of n-3 Fatty Acids in Nile Tilapia: a Factorial Design

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Journal of the American Oil Chemists' Society

Abstract

A 22 factorial design (two factors at two levels, in triplicate) was performed to investigate the influence of factors A (time of treatment, 15 and 30 days) and B (chia oil content in a supplemented diet, at 2.1 and 4.2 %) in three responses of interest referring to: (a) the incorporation of alpha-linolenic acid (LNA) in lipids of Nile tilapia fillet; (b) the enhancement of n-3 fatty acids; and (c) the decrease in the omega-6/omega3 (n-6/n-3) ratio in fish. Factors A and B were significant in the three regression models obtained and the interaction AB was a significant contributor to the LNA and n-6/n-3 ratio. Analysis of variance suggested three significant and predictive mathematical models. Response surfaces analyses from designs indicated higher LNA and n-3 contents and a lower n-6/n-3 ratio using both factors A and B in the higher levels (30 days of treatment and 4.2 % of chia oil in the diet for fish) chosen for this study.

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References

  1. Franco MLRS, Franco NP, Gasparino E, Dorado DM, Prado M, Vesco APD (2013) Comparação das peles de tilápia do Nilo, Pacu e Tambaqui: histologia, composição e resistência. Arch Zootec 62:21–32

    Article  Google Scholar 

  2. Carbonera F, Santos HMC, Montanher PF, Schneider VVA, Lopes AP, Visentainer JV (2014) Distinguishing wild and farm-raised freshwater fish through fatty acid composition: application of statistical tools. Eur J Lipid Sci Technol 116:1363–1371

    Article  CAS  Google Scholar 

  3. Visentainer JV, Souza NE, Makoto M, Hayashi C, Franco MRB (2005) Influence of diets enriched with flaxseed oil on the alpha-linolenic, eicosapentaenoic and docosahexaenoic fatty acid in Nile tilapia (Oreochromis niloticus). Food Chem 90:557–560

    Article  CAS  Google Scholar 

  4. Silva BC, Santos HMC, Montanher PF, Boeing JS, Almeida VC, Visentainer JV (2014) Incorporation of omega-3 fatty acids in Nile Tilapia (Oreochromis niloticus) Fed Chia (Salvia hispanica L.) Bran. J Am Oil Chem Soc 91:429–437

    Article  Google Scholar 

  5. Carbonera F, Bonafé EG, Martin CA, Montanher PF, Ribeiro RP, Figueiredo LC, Almeida VC, Visentainer JV (2014) Effect of replacement of sunflower oil with perilla oil on the absolute fatty acid composition in Nile Tilapia (GIFT). Food Chem 148:230–234

    Article  CAS  Google Scholar 

  6. Santos HMC, Nishiyama MF, Bonafe EG, Oliveira CAL, Matsushita M, Visentainer JV, Ribeiro RP (2014) Influence of a diet enriched with perilla seed bran on the composition of omega-3 fatty acid in Nile tilapia. J Am Oil Chem Soc 91:1939–1948

    Article  Google Scholar 

  7. Ayerza R (2011) The seed’s oil content and fatty acid composition of chia (Salvia hispanica L.) var. iztac 1, grown under six tropical ecosystems conditions. Interciencia 36:620–624

    Google Scholar 

  8. Barros Neto B, Scarminio IS, Bruns RE (2003) Como fazer experimentos—pesquisa e desenvolvimento na ciência e na indústria, 2nd edn. Unicamp, Campinas

    Google Scholar 

  9. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  10. Hartman L, Lago RCA (1973) Rapid preparation of fatty acid methyl esters from lipids. Lab Pract 22:475–477

    CAS  Google Scholar 

  11. Maia EL, Rodriguez-Amaya DB (1993) Avaliação de um método simples e econômico para a metilação de ácidos graxos com lipídios de diversas espécies de peixes. Rev Inst Adolfo Lutz 53:27–35

    CAS  Google Scholar 

  12. Martin CA, Oliveira CC, Visentainer JV, Matsushita M, Souza NE (2008) Optimization of the selectivity of a cyanopropyl stationary phase for the gas chromatographic analysis of trans fatty acids. J Chromatogr A 1194:111–117

    Article  CAS  Google Scholar 

  13. Joseph JD, Ackman RG (1992) Capillary column gas chromatography method for analysis of encapsulated fish oil and fish oil ethyl esters: collaborative study. J AOAC Int 75:488–506

    CAS  Google Scholar 

  14. Visentainer JV (2012) Aspectos analíticos da resposta do detector de ionização em chama para ésteres de ácidos graxos em biodiesel e alimentos. Quim Nova 35:274–279

    Article  CAS  Google Scholar 

  15. Department of Health and Social Security (1984) Diet and cardiovascular disease: report on health and social subjects, London

  16. Youdim KA, Martin A, Joseph JA (2000) Essential fatty acids and the brain: possible health implications. Int J Devl Neuroscience 18:383–399

    Article  CAS  Google Scholar 

  17. Montgomery DC, Peck EA, Vining GG (2001) Introduction to linear regression analysis, 3rd edn. Wiley, New York

    Google Scholar 

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Acknowledgments

The authors are grateful for the support of Federal University of Technology-Paraná and State University of Maringá in conducting this research.

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Authors and Affiliations

  1. Federal University of Technology-Paraná, Marcílio Dias Street, 635, 86812-460, Apucarana, PR, Brazil

    Vanessa Vivian Almeida Schneider

  2. Post-Graduate Program of Food Science, State University of Maringá, 87020-900, Maringá, PR, Brazil

    Vanessa Vivian Almeida Schneider

  3. Department of Chemistry, State University of Maringá, 87020-900, Maringá, PR, Brazil

    Fabiana Carbonera, Paula Fernandes Montanher, Ana Paula Lopes, Makoto Matsushita & Jesuí Vergílio Visentainer

Authors
  1. Vanessa Vivian Almeida Schneider
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  2. Fabiana Carbonera
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  3. Paula Fernandes Montanher
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  4. Ana Paula Lopes
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  5. Makoto Matsushita
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  6. Jesuí Vergílio Visentainer
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Corresponding author

Correspondence to Vanessa Vivian Almeida Schneider.

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Schneider, V.V.A., Carbonera, F., Montanher, P.F. et al. Incorporation of Alpha-Linolenic Acid and Enhancement of n-3 Fatty Acids in Nile Tilapia: a Factorial Design. J Am Oil Chem Soc 92, 693–700 (2015). https://doi.org/10.1007/s11746-015-2632-5

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  • DOI: https://doi.org/10.1007/s11746-015-2632-5

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