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Unintended polar metabolite profiling of carotenoid-biofortified transgenic rice reveals substantial equivalence to its non-transgenic counterpart

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Abstract

Substantial equivalence is a critical concept for biosafety assessment of genetically modified (GM) crops. To investigate substantial equivalence among carotenoid-biofortified GM rice and five conventional rice cultivars having common white (three) and red (two) grain colors, profiles of 52 polar metabolites were analyzed using gas chromatography time-of-flight mass spectrometry. The results were compared to evaluate the differences among GM and non-GM rice cultivars using principal components analysis. The GM rice is more comparable to its non-transgenic counterpart rice variety according to the closer co-separation than for other cultivars tested. This suggests that profiling of unintended polar metabolites could be a useful tool to reveal substantial equivalence of GM rice.

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Acknowledgments

This work was supported by grants from the National Academy of Agricultural Science (Code PJ008484), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Jae Kwang Kim, Soo-Yun Park, Si Myung Lee, Sun-Hyung Lim, Hyo Jin Kim, Sung-Dug Oh, Yunsoo Yeo, Hyun Suk Cho & Sun-Hwa Ha

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  1. Jae Kwang Kim
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  2. Soo-Yun Park
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  3. Si Myung Lee
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  4. Sun-Hyung Lim
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  5. Hyo Jin Kim
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Correspondence to Sun-Hwa Ha.

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Kim, J.K., Park, SY., Lee, S.M. et al. Unintended polar metabolite profiling of carotenoid-biofortified transgenic rice reveals substantial equivalence to its non-transgenic counterpart. Plant Biotechnol Rep 7, 121–128 (2013). https://doi.org/10.1007/s11816-012-0231-6

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  • DOI: https://doi.org/10.1007/s11816-012-0231-6

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