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CHLORIDE CHANNEL 1 promotes drought tolerance in rice, leading to increased grain yield

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Abstract

Plant chloride channels (CLCs) localize to the plasma and organellar membranes; these channels play pivotal roles in the modulation of ion homeostasis and cell turgor. Recent studies have shown that the expression of CLCs is involved in plant responses to environmental stress. Here, we examined the rice (Oryza sativa) tonoplast-localized channel OsCLC1. OsCLC1 is preferentially expressed in roots, and therefore, we generated transgenic rice with root-specific overexpression of OsCLC1 (RCc3::OsCLC1). We also identified a T-DNA mutant line that lacks expression of OsCLC1 (osclc1). We found that RCc3::OsCLC1 rice plants showed increased tiller number and grain yield, whereas the osclc1 plants exhibited decreased tiller number and grain yield, compared with wild type. These observations suggest that expression of OsCLC1 affects rice growth and productivity. Furthermore, RCc3::OsCLC1 plants showed enhanced drought tolerance, leading to increased grain yield compared to wild-type plants grown under the same conditions. By contrast, osclc1 mutants exhibited reduced drought tolerance and productivity compared to wild-type plants. When expression of OsCLC1 was analyzed in drought, jasmonic acid (JA) or abscisic acid (ABA)-treated rice, expression of OsCLC1 was preferentially upregulated in roots in response to drought and JA, and was preferentially upregulated in shoots in response to ABA. Together with the finding that expression of OsCLC1 is positively correlated both with expressions of OsDREB1A and OsbHLH148, key transcription factors in drought and JA responses, respectively, these results suggest that OsCLC1 regulates drought tolerance in rice and JA signaling is involved in this process.

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Acknowledgements

We heartily thank Prof. G. An for donating osclc1 mutant seeds. This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project no. PJ01121501 to Y. D. C. & PJ01364301 to G. J.) Rural Development Administration, Republic of Korea, through the National Center for GM Crops, and the National Research Foundation of Korea Grant funded by the Korean Government (MOE) [NRF-2016R1D1A1B03931167]. A graduate research assistantship to T. Y. U. and S. L. from the Brain Korea 21 Plus project of the MOE is also acknowledged.

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  1. Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea

    Tae Young Um, Sangyool Lee, Geupil Jang & Yang Do Choi

  2. Graduate School of International Agricultural Technology and Crop Biotechnology Institute/Green BioScience and Technology, Seoul National University, Pyeongchang, 25354, South Korea

    Ju-Kon Kim

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Correspondence to Yang Do Choi.

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Um, T.Y., Lee, S., Kim, JK. et al. CHLORIDE CHANNEL 1 promotes drought tolerance in rice, leading to increased grain yield. Plant Biotechnol Rep 12, 283–293 (2018). https://doi.org/10.1007/s11816-018-0492-9

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