Abstract
Glycerol is a renewable resource for it is formed as a byproduct during biodiesel production. Because of its large volume production, it seems to be a good idea to develop a technology that converts this waste into products of high value, for example, to 1,3-propanediol (1,3-PD). We suggested an enzymatic bioconversion in a membrane reactor in which the NAD coenzyme can be regenerated, and three key enzymes are retained by a 10-kDa ultrafilter membrane. Unfortunately, some byproducts also formed during successful glycerol to 1,3-PD bioconversion runs, as we used crude enzyme solution of Klebsiella pneumoniae. To study the possibilities to avoid this byproduct formation, we built a mathematical description of this system. The model was also used for simulation bioconversions of high glycerol concentration with and without elimination of byproduct formation and of continuous operation.
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Abbreviations
- 1,3-PD:
-
1,3-propanediol
- DHA:
-
1,3-dihydroxyacetone
- GDH:
-
glycerol dehydrogenase enzyme
- GDHt:
-
glycerol dehydratase enzyme
- PDOR:
-
1,3-propanediol oxydoreductase
- DHAK:
-
dihydroxyacetone kinase
- TPI:
-
triose-phosphate isomerase
- GAPD:
-
glyceraldehyde-3-phosphate dehydrogenase
- PGK:
-
phosphoglycerate kinase
- PGM:
-
phosphoglycerate mutase
- PPH:
-
phosphopyruvate hydratase
- PK:
-
pyruvate kinase
- PS:
-
pyruvate synthase
- PAT:
-
phosphate acetyltransferase
- PTA:
-
phospholipid-translocating ATPase
- LDH:
-
lactate-dehydrogenase
- LMO:
-
lactate 2-monooxygenase
- BB:
-
“black-box”
- Clha:
-
chloro-3-hydroxyacetone
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Acknowledgment
We are thankful to OTKA T032015 and NKFP-3/A/0035/2002 for the financial support of our work.
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Németh, Á., Sevella, B. Development of a New Bioprocess for Production of 1,3-propanediol I.: Modeling of Glycerol Bioconversion to 1,3-propanediol with Klebsiella pneumoniae Enzymes. Appl Biochem Biotechnol 144, 47–58 (2008). https://doi.org/10.1007/s12010-007-0040-5
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DOI: https://doi.org/10.1007/s12010-007-0040-5