Skip to main content
SpringerLink
Log in

A Novel α-Glucosidase Inhibitor Protein from the Rhizomes of Zingiber ottensii Valeton

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The objective of this study was to investigate a new protein with α-glucosidase inhibitory activity from the rhizomes of Zingiber ottensii. With a simple salting-out technique followed by single-step anion-exchange purification, the protein was successfully purified from the rhizomes. This protein was found to have three likely sub-unit types, 32.5, 15.2, and 13.8 kDa, as revealed by native and reducing SDS-PAGE analysis. Determination of the kinetics of the inhibition of α-glucosidase from Saccharomyces cerevisiae by standard enzymatic methods indicated the maximum percent inhibition; IC50 and K i of this protein were 77.5%, 30.15 μg/ml, and 140 μmol, while the K m and V max were 2.35 μmol and 0.11 mM/min, respectively. The inhibitory action was pH-independent within the pH range 2–10, but was potentially affected by buffer salts, and was relatively temperature-stable between 4–35 °C, with a maximum activity at 65 °C. The amino acid sequence of an internal fragment of this purified Z. ottensii rhizomal protein had a similarity to the sequence from the plant cysteine proteinase family. Although this α-glucosidase inhibitory protein was purified from Z. ottensii rhizomes and preliminarily characterized, further studies are needed prior to firm applications being envisaged.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Park, H., Hwang, K. Y., Kim, Y. H., Oh, K. H., Lee, J. Y., & Kim, K. (2008). Bioorganic & Medicinal Chemistry Letters, 18, 3711–3715.

    Article  CAS  Google Scholar 

  2. Gao, H., & Kawabata, J. (2005). Bioorganic & Medicinal Chemistry, 13, 1661–1671.

    Article  CAS  Google Scholar 

  3. Zhu, Y. P., Yin, L. J., Cheng, Y. Q., Yamaki, K., Mori, Y., Su, Y. C., et al. (2008). Food Chemistry, 109, 737–742.

    Article  CAS  Google Scholar 

  4. Taylor, J. R. N. (1994). Journal of the Institute of Brewing, 100, 417–419.

    CAS  Google Scholar 

  5. De Melo, E. B., Gomes, A. D., & Carvalho, I. (2006). Tetrahedron, 62, 10277–10302.

    Article  Google Scholar 

  6. Whitby, K., Pierson, T., Geiss, B., Lane, K., Engle, M., Zhou, Y., et al. (2005). Journal of Virology, 79, 8698–8706.

    Article  CAS  Google Scholar 

  7. Iwata, H., Suzuki, T., Takahashi, K., & Aramaki, I. (2002). Journal of Bioscience and Bioengineering, 93, 296–302.

    Article  CAS  Google Scholar 

  8. Malá, Š., Karasová, P., Marková, M., & Králová, B. (2001). Czech Journal of Food Sciences, 19, 57–61.

    Google Scholar 

  9. Ladas, S., Frydas, A., Papadopoulos, A., & Raptis, S. (1992). Gut, 33, 1246–1248.

    Article  CAS  Google Scholar 

  10. Evan, S. V., Gatehouse, A. M. R., & Fellows, L. E. (1985). Entomologia Experimentalis et Applicata, 37, 257–261.

    Article  Google Scholar 

  11. Lee, D. (2000). Journal of Bioscience and Bioengineering, 89, 271–273.

    Article  CAS  Google Scholar 

  12. Lee, D., & Lee, S. (2001). FEBS Letter, 501, 84–86.

    Article  CAS  Google Scholar 

  13. Jong-Anurakkun, N., Bhandari, M. R., & Kawabata, J. (2007). Food Chemistry, 103, 1319–1323.

    Article  CAS  Google Scholar 

  14. Yogisha, S., & Raveesha, K. A. (2009). Pharmacologyonline, 1, 404–409.

    Google Scholar 

  15. Sancheti, S., Sancheti, S., & Seo, S. U. (2009). American Journal of Pharmacology and Toxicology, 4, 8–11.

    Article  Google Scholar 

  16. Tunsaringkarn, T., Rungsiyothin, A., & Rungrungsi, N. (2009). The Public Health Journal of Burapha University, 4, 54–63.

    Google Scholar 

  17. Van de Laar, F., Lucassen, P., Akkermans, R., Van de Lisdonk, E., Rutten, G., & Van Weel. (2005). Diabetes Care, 28, 166–175.

    Google Scholar 

  18. Tsujimoto, T., Shioyama, E., Moriya, K., Kawaratani, H., Shirai, Y., Toyohara, M., et al. (2008). World Journal of Gastroenterology, 14, 6087–6092.

    Article  Google Scholar 

  19. Ravindran, P., & Babu, K. (2005). In ginger: The genus Zingiber. USA: CRC Press.

    Google Scholar 

  20. Akiyama, K., Kikuzaki, H., Aoki, T., Okuda, A., Lajis, N., & Nakatani, N. (2006). Journal of Natural Products, 69, 1637–1640.

    Article  CAS  Google Scholar 

  21. Boukouvalas, J., & Wang, J. (2008). Organic Letters, 10, 3397–3399.

    Article  CAS  Google Scholar 

  22. Tiptara, P., Petsom, A., Roengsumran, S., & Sangvanich, P. (2008). Journal of the Science of Food and Agriculture, 88, 1025–1034.

    Article  CAS  Google Scholar 

  23. Samarkina, O., Popova, A., Gvozdik, E., Chkalina, V., Zvyagin, I., Rylova, Y., et al. (2009). Protein Expression and Purification, 65, 108–113.

    Article  CAS  Google Scholar 

  24. Tipthara, P., Sangvanich, P., Macth, M., & Petsom, A. (2007). Journal of Plant Biology, 50, 167–173.

    Article  CAS  Google Scholar 

  25. Bollag, D. M., Rozycki, M. D., & Edelstein, S. J. (1996). In protein methods (2nd ed.). Wiley-Liss, Inc: New York.

    Google Scholar 

  26. Lamelli, U. K. (1970). Nature, 227, 680–685.

    Article  Google Scholar 

  27. Mortz, E., Vorm, O., Mann, M., & Roepstorff, P. (1994). Biological Mass Spectrometry, 23, 249–261.

    Article  CAS  Google Scholar 

  28. Wang, H., & Ng, T. (2003). Protein Expression and Purification, 28, 9–14.

    Article  CAS  Google Scholar 

  29. Lee, H. (2005). Journal of Agricultural and Food Chemistry, 53, 2446–2450.

    Article  CAS  Google Scholar 

  30. Boonmee, A., Reynolds, C., & Sangvanich, P. (2007). Planta Medica, 73, 1197–1201.

    Article  CAS  Google Scholar 

  31. Ahmed, K. S. O. H., Milosavić, N. B., Popović, M. M., Prodanović, R. M., Knežević, Z. D., & Jankov, R. M. (2007). Journal of the Serbian Chemical Society, 72, 1255–1263.

    Article  CAS  Google Scholar 

  32. Agrawal, P. B., & Pandit, A. B. (2003). Biochemical Engineering Journal, 15, 37–45.

    Article  CAS  Google Scholar 

  33. Shevchenko, A., Sunyaev, S., Loboda, A., Shevchenko, A., Bork, P., Ens, W., et al. (2001). Analytical Chemistry, 73, 1917–1926.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank the 90th Anniversary of Chulalongkorn University fund for financial support of this research. The Institute of Biotechnology and Genetic Engineering and Biotechnology program, the Faculty of Science, Chulalongkorn University, are both acknowledged for support and facilities. We also thank Dr. Robert Butcher (Publication Counselling Unit, Chulalongkorn University) for his constructive comments in preparing this manuscript.

Author information

Authors and Affiliations

  1. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Nathachai Tiengburanatam

  2. Research Center for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Apaporn Boonmee & Polkit Sangvanich

  3. The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand

    Polkit Sangvanich & Aphichart Karnchanatat

Authors
  1. Nathachai Tiengburanatam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Apaporn Boonmee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Polkit Sangvanich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aphichart Karnchanatat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aphichart Karnchanatat.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tiengburanatam, N., Boonmee, A., Sangvanich, P. et al. A Novel α-Glucosidase Inhibitor Protein from the Rhizomes of Zingiber ottensii Valeton. Appl Biochem Biotechnol 162, 1938–1951 (2010). https://doi.org/10.1007/s12010-010-8971-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-010-8971-7

Keyword

Search

Navigation