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Enhanced Enzymatic Hydrolysis of Waste Paper for Ethanol Production Using Separate Saccharification and Fermentation

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Abstract

Ethanol produced from lignocellulosic biomass is a renewable alternative to diminishing petroleum-based liquid fuels. In this study, the feasibility of ethanol production from waste paper using the separate hydrolysis and fermentation (SHF) was investigated. Two types of waste paper materials, newspaper and office paper, were evaluated for their potential to be used as a renewable feedstock for the production of fermentable sugars via enzymatic hydrolysis of their cellulose fractions. Hydrolysis step was conducted with a mixture of cellulolytic enzymes produced locally by Trichoderma reesei Rut-C30 (cellulase-overproducing mutant) and Aspergillus niger F38 cultures. Surfactant pretreatment effect on waste paper enzymatic digestibility was studied and Triton X-100 at 0.5 % (w w−1) has improved the digestibility of newspaper about 45 %. The effects of three factors (dry matter quantity, phosphoric acid pretreatment and hydrolysis time) on the extent of saccharification were also assessed and quantified by using a methodical approach based on response surface methodology. Under optimal hydrolysis conditions, maximum degrees of saccharification of newspaper and office paper were 67 and 92 %, respectively. Sugars released from waste paper were subsequently converted into ethanol (0.38 g ethanol g−1 sugar) with Saccharomyces cerevisiae CTM-30101.

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Acknowledgments

This work received financial support from the Ministry of Higher Education and Scientific Research, Tunisia, granted to the Laboratory of Biomass Valorisation and Protein Production in Eukaryotes (Centre of Biotechnology of Sfax, CBS, Sfax, Tunisia). We would like to express our gratitude to Pr. Hafedh Dhouib, responsible of the National Strains Collection of CBS, Tunisia, who gave us the yeast strain for fermentation. Special thanks are also due to Mr. Nabil Zouari, PHD student at ENIS Sfax, Tunisia, for his help in figure treatment.

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Authors and Affiliations

  1. Laboratory of Biomass Valorisation and Protein Production in Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3038, Sfax, Tunisia

    Mohamed Guerfali, Adel Saidi, Ali Gargouri & Hafedh Belghith

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  1. Mohamed Guerfali
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  2. Adel Saidi
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  3. Ali Gargouri
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  4. Hafedh Belghith
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Correspondence to Mohamed Guerfali.

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Guerfali, M., Saidi, A., Gargouri, A. et al. Enhanced Enzymatic Hydrolysis of Waste Paper for Ethanol Production Using Separate Saccharification and Fermentation. Appl Biochem Biotechnol 175, 25–42 (2015). https://doi.org/10.1007/s12010-014-1243-1

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  • DOI: https://doi.org/10.1007/s12010-014-1243-1

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