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The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart

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Abstract

Lactate dehydrogenase (LDH, EC1.1.1.27), widely expressed in the heart, liver, and other tissues, plays an important role in glycolysis and glyconeogenesis. The activity of LDH is often altered upon inflammatory responses in animals. Nano-TiO2 was shown to provoke various inflammatory responses both in rats and mice; however, the molecular mechanism by which TiO2 exerts its toxicity has not been completely understood. In this report, we investigated the mechanisms of nano-anatase TiO2 (5 nm) on LDH activity in vitro. Our results showed that LDH activity was greatly increased by low concentration of nano-anatase TiO2, while it was decreased by high concentration of nano-anatase TiO2. The spectroscopic assays revealed that the nano-anatase TiO2 particles were directly bound to LDH with mole ratio of [nano-anatase TiO2] to [LDH] was 0.12, indicating that each Ti atom was coordinated with five oxygen/nitrogen atoms and a sulfur atoms of amino acid residues with the Ti–O(N) and Ti–S bond lengths of 1.79 and 2.41 Å. We postulated that the bound nano-anatase TiO2 altered the secondary structure of LDH, created a new metal ion-active site for LDH, and thereby enhanced LDH activity.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 20671067) and by the Medical Development Foundation of Suzhou University (grant no. EE120701) and by the National New Ideas Foundation of Student (grant no. 57315427).

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Authors and Affiliations

  1. Medical College of Soochow University, Suzhou, 215123, People’s Republic of China

    Yanmei Duan, Huiting Liu, Jinfang Zhao, Chao Liu, Jinying Yan, Linglan Ma, Jie Liu, Jie Ruan & Fashui Hong

  2. Nanotechnology Center, University of Arkansas, Little Rock, AR, 72204, USA

    Zhongrui Li

  3. Synchrotron Radiation Laboratory, Institute of High Energy Physics, The Chinese Academy of Science, Beijing, 100039, People’s Republic of China

    Yaning Xie

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  1. Yanmei Duan
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  2. Huiting Liu
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  3. Jinfang Zhao
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  4. Chao Liu
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  5. Zhongrui Li
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  6. Jinying Yan
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  7. Linglan Ma
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  9. Yaning Xie
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  10. Jie Ruan
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  11. Fashui Hong
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Corresponding author

Correspondence to Fashui Hong.

Additional information

Y. Duan, H. Liu, and J. Zhao contributed equally to this work.

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Duan, Y., Liu, H., Zhao, J. et al. The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart. Biol Trace Elem Res 130, 162–171 (2009). https://doi.org/10.1007/s12011-009-8326-9

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  • DOI: https://doi.org/10.1007/s12011-009-8326-9

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