Abstract
We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV–vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding.
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Abbreviations
- CD:
-
Circular dichroism
- Col:
-
Colemanite
- CT-DNA:
-
Calf thymus DNA
- FT-IR:
-
Fourier transform infrared
- Ule:
-
Ulexite
- UV–vis:
-
Ultraviolet–visible
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Acknowledgments
The authors are thankful to the Turkish National Boron Research Institute (Ulusal Bor Araştırma Enstitüsü (BOREN), Ankara, Turkey) for financial support through Research Grant No. 2012.ç0356 and to E. Kalyoncu, A. D. Özkan, and R. T. Gursacli for their help and support in conducting the experiments described. A. Ozdemir is supported by TUBITAK BIDEB (2211) PhD fellowship. O.F. Sarioglu acknowledges TUBITAK BIDEB (2211-C) for National PhD Scholarship.
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Ozdemir, A., Sarioglu, O.F. & Tekinay, T. Spectroscopic Evaluation of DNA–Borate Interactions. Biol Trace Elem Res 168, 508–515 (2015). https://doi.org/10.1007/s12011-015-0369-5
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DOI: https://doi.org/10.1007/s12011-015-0369-5