Abstract
This study was performed to evaluate the effect of concomitant supplementation of genistein and silicon on bone mineral density and bone metabolism-related markers in ovariectomized rat. Three-month-old Sprague Dawley female rats were subjected to bilateral ovariectomy (OVX) or sham surgery, and then the OVX rats were randomly divided into four groups: OVX-GEN, OVX-Si, OVX-GEN-Si, and OVX. Genistein and silicon supplementation was started immediately after OVX and continued for 10 weeks. In the OVX-GEN group, 5 mg genistein per gram body weight was injected subcutaneously. The OVX-Si group was given soluble silicon daily in demineralized water (Si 20 mg/kg body weight/day). The OVX-GEN-Si group was given subcutaneous injections of 5 mg genistein per gram body weight, at the same time, given soluble silicon daily (Si 20 mg/kg body weight/day). The results showed that the genistein supplementation in the OVX rats significantly prevented the loss of uterus weight; however, the silicon supplementation showed no effect on the uterus weight loss. The lumbar spine and femur bone mineral density was significantly decreased after OVX surgery; however, this decrease was inhibited by the genistein and/or silicon, and the BMD of the lumbar spine and femur was the highest in the OVX-GEN-Si-treated group. Histomorphometric analyses showed that the supplementation of genistein and/or silicon restored bone volume and trabecular thickness of femoral trabecular bone in the OVX group. Besides, the treatment with genistein and silicon for 10 weeks increased the serum levels of calcium and phosphorus in the OVX rats; serum calcium and serum phosphorus in the OVX-GEN-Si group were higher than those in the OVX-GEN and OVX-Si group (P < 0.05). At the same time, the treatment with genistein and/or silicon decreased serum alkaline phosphatase (ALP) and osteocalcin, which were increased by ovariectomy; serum ALP and osteocalcin in the OVX-GEN-Si group were lower than those in the OVX-GEN and OVX-Si groups (P < 0.05). The results above indicate that genistein and silicon have synergistic effects on bone formation in ovariectomized rats.
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References
Lecart MP, Reginster JY (2011) Current options for the management of postmenopausal osteoporosis. Expert Opin Pharmacother 12:2533–2552
Rosen CJ (2005) Clinical practice. Postmenopausal osteoporosis. N Engl J Med 353:595–603
Umland EM, Rinaldi C, Parks SM et al (1999) The impact of estrogen replacement therapy and raloxifene on osteoporosis, cardiovascular disease, and gynecologic cancers. Ann Pharmacother 33:1315–1328
Seckin B, Pekcan MK, Inal HA et al (2016) The relationship between breast density and bone mineral density in never users of postmenopausal hormone therapy. Aging Clin Exp Res 7:1–5
Ersen AP, Karsdal MA et al (2007) Hormone replacement as the first-line prevention for postmenopausal osteoporosis. Expert Rev Obstet Gynecol 2:689–700
Lagari VS, Levis S (2014) Phytoestrogens for menopausal bone loss and climacteric symptoms. J Steroid Biochem Mol Biol 139:294–301
Arjmandi BH, Birnbaum R, Goyal NV et al (1998) Bone-sparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. Am J Clin Nutr 68:1364S–1368S
Blum SC, Heaton SN, Bowman BM et al (2003) Dietary soy protein maintains some indices of bone mineral density and bone formation in aged ovariectomized rats. J Nutr 133:1244–1249
Fu SW, Zeng GF, Zong SH et al (2014) Systematic review and meta-analysis of the bone protective effect of phytoestrogens on osteoporosis in ovariectomized rats. Nutr Res 34:467–477
Jugdaohsingh R (2007) Silicon and bone health. J Nutr Health Aging 11:99–110
Carlisle EM (1976) In vivo requirement for silicon in articular cartilage and connective tissue formation in the chick. J Nutr 106:478–484
Carlisle EM (1972) Silicon: an essential element for the chick. Science 178:619–621
Bae YJ, Kim JY, Choi MK, Chung YS, Kim MH (2008) Short-term administration of water-soluble silicon improves mineral density of the femur and tibia in ovariectomized rats. Biol Trace Elem Res 124:157–163
Kim MH, Bae YJ, Choi MK, Chung YS (2009) Silicon supplementation improves the bone mineral density of calcium-deficient ovariectomized rats by reducing bone resorption. Biol Trace Elem Res 128:239–247
Jugdaohsingh R, Watson AIE, Bhattacharya P et al (2015) Positive association between serum silicon levels and bone mineral density in female rats following oral silicon supplementation with monomethyl silane triol. Osteoporos Int 26:1405–1415
Yang L, Yu Z, Qu H et al (2014) Comparative effects of hispidulin, Genistein, and Icariin with Estrogen on Bone Tissue in Ovariectomized Rats. Cell Biochem Biophys 70:485–490
Dai R, Ma Y, Sheng Z et al (2008) Effects of genistein on vertebral trabecular bone microstructure, bone mineral density, microcracks, osteocyte density, and bone strength in ovariectomized rats. J Bone Miner Metab 26:342–349
Seaborn CD, Nielsen FH (1994) Effects of germanium and silicon on bone mineralization. Biol Trace Elem Res 42:151–164
Seaborn CD, Nielsen FH (2002) Dietary silicon and arginine affect mineral element composition of rat femur and vertebra. Biol Trace Elem Res 89:239–250
Hott M, de Pollak C, Modrowski D, Marie PJ (1993) Short-term effects of organic silicon on trabecular bone in mature ovariectomized rats. Calcif Tissue Int 53:174–179
Rico H, Gallego-Lago JL, Hernández ER et al (2000) Effect of silicon supplement on osteopenia induced by ovariectomy in rats. Calcif Tissue Int 66:53–55
Calomme M, Geusens P, Demeester N et al (2006) Partial prevention of long-term femoral bone loss in aged ovariectomized rats supplemented with choline-stabilized orthosilicic acid. Calcif Tissue Int 78:227–232
Kayongo-Male H, Julson JL (2008) Effects of high levels of dietary silicon on bone development of growing rats and turkeys fed semi-purified diets. Biol Trace Elem Res 123:191–201
Levis S, Strickman-Stein N, Ganjei-Azar P et al (2011) Soy isoflavones in the prevention of menopausal bone loss and menopausal symptoms: a randomized, double-blind trial. Arch Intern Med 171:1363–1369
Duncan AM, Phipps WR, Kurzer MS (2003) Phytooestrogens. Best Pract Res Clin Endocrinol Metab 17:253–271
Zhang X, Shu XO, Li H et al (2005) Prospective cohort study of soy food consumption and risk of bone fracture among postmenopausal women. Arch Intern Med 165:1890–1895
Gutendorf B, Westendorf J (2001) Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens. Toxicology 166:79–89
D’Anna R, Cannata ML, Atteritano M et al (2007) Effects of the phytoestrogen genistein on hot flushes, endometrium, and vaginal epithelium in postmenopausal women: a 1-year randomized, double-blind, placebo-controlled study. Menopause 14:648–655
Li B, Yu S (2003) Genistein prevents bone resorption diseases by inhibiting bone resorption and stimulating bone formation. Biol Pharm Bull 26:780–786
Fanti P, Monier-Faugere MC, Geng Z et al (1998) The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats. Osteoporos Int 8:274–281
Liao MH, Tai YT et al (2014) Genistein induces oestrogen receptor alpha gene expression in osteoblasts through the activation of mitogen-activated protein kinases/NF-kappaB/activator protein-1 and promotes cell mineralisation. Br J Nutr 111:55–63
Wang ZL, Sun JY, Wang DN et al (2006) Pharmacological studies of the large-scaled purified genistein from Huaijiao (Sophora japonica-Leguminosae) on anti-osteoporosis. Phytomedicine. Int J Phytother Phytopharmacol 13:718–723
Acknowledgments
This work was supported by the Qinling-Bashan Mountains Bioresources Comprehensive Development, Collaborative Innovation Center Research Funds QBXT-Z(P)-15-18 and QBXT-Z(P)-15-25, the Natural Science Foundation of Shaanxi Provincial Science and Technology Department 2015JQ8305, the Shaanxi Provincial Science Association youth talent founds 20160128, Postdoctoral Scientific Research Project of Shaanxi Sci-tech University SLGBH16-03, and Doctoral Research Project of Shaanxi Sci-tech University SLGQD 14-01.
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All experimental procedures complied with the criteria in Guide for the Care and Use of Laboratory Animals, and this study was approved by the institutional ethics committee.
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Qi, S., Zheng, H. Combined Effects of Phytoestrogen Genistein and Silicon on Ovariectomy-Induced Bone Loss in Rat. Biol Trace Elem Res 177, 281–287 (2017). https://doi.org/10.1007/s12011-016-0882-1
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DOI: https://doi.org/10.1007/s12011-016-0882-1