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Sequential Extraction as Novel Approach to Compare 12 Medicinal Plants From Kenya Regarding Their Potential to Release Chromium, Manganese, Copper, and Zinc

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Abstract

This study is focusing on a novel approach to screen a large number of medicinal plants from Kenya regarding their contents and availability of selected metals potentially relevant for treatment of diabetes patients. For this purpose, total levels of zinc, chromium, manganese, and copper were determined by flame atomic absorption spectrometry and inductively coupled plasma mass spectrometry as well as BCR sequential extraction to fractionate the elemental species in anti-diabetic medicinal plants collected from five natural locations in two sub counties in Nyamira County, Kenya. Solanum mauense had the highest zinc level of 123.0 ± 3.1 mg/kg while Warburgia ugandensis had the lowest level of 13.9 ± 0.4 mg/kg. The highest level of copper was in Bidens pilosa (29.0 ± 0.6 mg/kg) while the lowest was in Aloe vera (3.0 ± 0.1 mg/kg). Croton macrostachyus had the highest manganese level of 1630 ± 40 mg/kg while Clerodendrum myricoides had the lowest (80.2 ± 1.2 mg/kg). The highest level of chromium was in Solanum mauense (3.20 ± 0.06 mg/kg) while the lowest (0.04 ± 0.01 mg/kg) were in Clerodendrum myricoides and Warburgia ugandesis among the medicinal plants from Nyamira and Borabu, respectively. The levels of the elements were statistically different from that of other elements while the level of a given element was not statistically different in the medicinal plants from the different sub counties. Sequential extraction was performed to determine the solubility and thus estimate the bioavailability of the four investigated essential and potentially therapeutically relevant metals. The results showed that the easily bioavailable fraction (EBF) of chromium, manganese, zinc, and copper ranged from 6.7 to 13.8%, 4.1 to 10%, 2.4 to 10.2%, and 3.2 to 12.0% while the potentially bioavailable fraction (PBF) ranged from 50.1 to 67.6%, 32.2 to 48.7%, 23.0 to 41.1%, and 34.6 to 53.1%, respectively. Bidens pilosa, Croton macrostachyus, Ultrica dioica, and Solanum mauense medicinal plants used to treat diabetes by 80 % of the herbalists in Nyamira County were found to be rich in chromium, manganese, copper, and zinc. The EBF of zinc, manganese, and chromium constitutes adequate amounts recommended for daily intake not exceeding the ADI and delivered a low percentage of RDA when estimating daily intake during therapy from typically applied doses. The plants did not show any significant differences at p < 0.05 in terms of concentrations of the elements between the two study areas though the levels of the different elements were statistically significant. Another major observation was that high total levels of the metals in a given plant did not necessarily translate to high bioavailable levels, and hence the need to determine bioavailable form as it is the one accessible to the patient.

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References

  1. Adongo SO, Murungi J, Wanjau R, Ndegwa F (2012) Determination of the concentrations of zinc, magnesium and iron in some medicinal plants used by the Chuka community in Kenya. J Sci Technol 1:1–7

    Article  Google Scholar 

  2. Mogwasi R (2016) Speciation of selected trace elements in medicinal plants from Nyamira County (Kenya). PhD Thesis, University of Nairobi

  3. Adedapo A, Jimoh F, Afolayan A (2011) Comparison of the nutritive value and biological activities of the acetone, methanol and water extracts of the leaves of Bidens pilosa and Chenopodium album. Acta Pol Pharm Drug Res 68:83–92

    Google Scholar 

  4. Kokwaro JO (1993) Medicinal plants of East Africa, Kenya Literature Bureau, Nairobi 20–490

  5. Majolagbe AO, Kuteyi V, Onwordi CT, Yusuf KA (2013) Concentration and bioavailability of iron in some selected blood-building medicinal plants in Southwest Nigeria. J Environ 2:19–24

    Google Scholar 

  6. Iakovidis I, Delimaris I, Piperakis MS (2011) Copper and its complexes in medicine: a biochemical approach. Mol Biol Int. doi:10.4061/2011/594529

  7. Kowol J, Kwapulinski J, Dopierala BB, Paukszto A, Bogunia M, Rochel R, Ahnert B (2011) Influence of a transboundary emission on bioavailability of metals of stinging nettle from the soil. Pol J Environ Stud 20:115–124

    CAS  Google Scholar 

  8. Turnlund JR (2006) Copper. In: Shils ME, Shike M, Ross AC, Caballero B, Cousins RJ (eds) Modern nutrition in health and disease, 10th Edited edn. Lippincott Williams & Wilkins, Philadelphia, pp 286–290

    Google Scholar 

  9. Konieczynski P, Wesolowski M (2007) Determination of zinc, iron, nitrogen and phosphorus in several botanical species of medicinal plants. Pol J Environ Stud 16:785–790

    CAS  Google Scholar 

  10. Nischwitz V, Mogwasi R, Zor S, Getenga Z, Kariuki DK, Gunther K (2017) A first comprehensive study total and hot water extractable fractions of selected elements in 19 medicinal plants from various locations in Nyamira County (Kenya). J Trace Elem Med Biol 39:54–61

    Article  CAS  PubMed  Google Scholar 

  11. Choudhury RP, Acharya R, Garg AN (2008) Availability of essential trace elements in medicinal herbs used for diabetes mellitus and their possible correlations. J Trace Elem Med Biol 45:36–52

    Google Scholar 

  12. Saba M, Radhakrishanan N, Lam KW, Arumugam G (2014) HPPA inhibitory effects of embelin and its metal complexes on diabetic complications: an approach with molecular docking studies. J Chem Pharm Res 6:679–682

    Google Scholar 

  13. Devereux M, Maccan M, Leon V, Geraghty M, Mckee V, Wikaira J (2000) Synthesis and bioactivity of manganese (II) complexes of phthalic and isophthalic acid. J Metal Based drugs 7:275–288

    Article  CAS  Google Scholar 

  14. Ansari T, Ikran N, Hag M, Fayyaz Q, Fayyaz I, Ghafoor I, Khalid N (2004) Essential trace metal (zinc, manganese, copper and iron) levels in plants of medicinal importance. J Biol Sci 4:95–99

    Article  Google Scholar 

  15. Garcia E, Cabrera C, Lorenzo ML, Lopez MC (2000) Chromium levels in spices and aromatic herbs. J Sci Total Environ 247:51–56

    Article  CAS  Google Scholar 

  16. Yang X, Dong F, Ren J, Sreejayan N (2006) Insulin-sensitizing and cholesterol-lowering effects of chromium. J Inorg Biochem 100:187–193

    Article  Google Scholar 

  17. Anna SM, Maja W, Wieslaw Z (2013) Multi-element analysis, bioavailability and fractionation of herbal tea products. J Braz Chem Soc 24

  18. Vartika R, Pramod KT, Sayyada K (2014) Effect of chromium on antioxidant potential of Catharanthus roseus varieties and production of their anticancer alkaloids: vincristine and vinblastine. Biomed Res Int. doi:10.1155/2014/934182

  19. Castro A (1998) Chromium in a series of Portuguese plants used in the herbal treatment of diabetes. J Trace Elem Med Biol 62:171–178

    Google Scholar 

  20. Kofi A, Asante IK, Asare C, Asare-Nkansah S, Bayor MT (2010) Profile of heavy metals in some medicinal plants from Ghana commonly used as components of herbal formulations. Pharm Res 2:41–44

    Google Scholar 

  21. Kokkotov E, Kanias G, Magdalini S (2009) Determination of trace and major elements in some medicinal herbs. Analyst 127:115–128

    Google Scholar 

  22. Paul RC, Garg AV (2007) Variation in essential trace and toxic elemental contents in Murnaya koenigii—a spice and medicinal herb from different Indian states. J Food Chem 104:1454–1463

    Article  Google Scholar 

  23. Rathore JS, Upadhyay M (2013) Investigation of zinc concentration in some medicinal plant leaves. Res J Pharm Sci 2:15–17

    Google Scholar 

  24. Raju NJG, Sarita P, Rao SCJ, Rao BCK, Reddy BS (2013) Correlation of trace elemental content in selected anticancer medicinal plants with their curative ability using particle induced x-ray emission (PIXE). J Med Plants Res 7:1081–1086

    Google Scholar 

  25. Masako H, Yoshiyuki S (2006) Anti-inflammatory and anti allergic activity of Bidens pilosa L.Var. radiate Scherff. J F Health Sci 52:711–717

    Article  Google Scholar 

  26. Oyeyiola AO, Olayinka KO, Alo BI (2009) Comparison of three sequential extraction procedures (SEP) for the speciation of potentially toxic metals in sediments of Lagos lagoon system. 4th annual research conference and fair, 2008, University of Lagos, Akoka 179

  27. Uduma AU, Jimoh WLO (2013) Sequential extraction procedure for portioning of lead, copper, cadmium, chromium and zinc in contaminated arable soils of Nigeria. Am J Environ Energy Power Res 1(9):186–208

    Google Scholar 

  28. Australian Government National Health and Medical Research Council (2002) Nutrient reference values for Australia and Newzealand

  29. Kenya Ministry of planning (2009) The Kenyan Census of 2009 report

  30. Brender JR, Hartman K, Nanga RPR (2010) Role of zinc in human islet amyloid polypeptide aggregation. J Am Chem Soc 132(26):8973–8983

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Queralt I, Ovejero M, Carvalho M, Marques A, Llabres J (2005) Quantitative determination of essential and trace element content of medicinal plants and their infusions by XRF and ICP techniques. X-ray Spec 34:213–217

    Article  CAS  Google Scholar 

  32. Basgel S, Erdemoglu SB (2006) Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey. Sci Total Environ 359:82–89

    Article  CAS  PubMed  Google Scholar 

  33. Leach RM, Harris ED (1997) Manganese. In: O’Dell BL, Sunde RA (eds) Handbook of nutritionally essential minerals. Marcel Dekker, New York, pp 335–355

    Google Scholar 

  34. Food and Nutrition Board, Institute of Medicine (2001) Manganese, dietary reference intakes for vitamin A, vitamin K, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. National Academy Press, Washington, D.C., pp 394–419

    Google Scholar 

  35. Kazi TG, Afridi HI, Kazi N (2008) Copper, chromium, manganese, iron, nickel, and zinc levels in biological samples of diabetes mellitus patients. Biol Trace Elem Res 122(1):1–18

    Article  CAS  PubMed  Google Scholar 

  36. Li YV (2014) Zinc and insulin in pancreatic beta-cells. Endocrine 45(2):178–189

    Article  CAS  PubMed  Google Scholar 

  37. Maret W (2013) Zinc biochemistry: from a single zinc enzyme to a key element of life. Adv Nutr 4(1):82–91

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Krystyna P (2007) Chemical speciation and fractionation of metals in wine. Chem Speciat Bioavailab 19(1):1–8

    Article  Google Scholar 

  39. Intawongse M, Dean JR (2008) Use of the physiologically-based extraction test to assess the oral bioaccessibility of metals in vegetable plants grown in contaminated soil. Environ Pollut 152:60–72

    Article  CAS  PubMed  Google Scholar 

  40. Cadkova Z, Szakova J, Miholova D, Horakova B, Kopecky O, Krivska D, Langrova I, Tlustos P (2015) Bioaccessibilty versus bioavailability of essential (Cu, Fe, Mn and Zn) and toxic (Pb) elements from phyto hyperaccumulator Pisti stratiotes: potential risk of dietary intake. J Agric Food Chem 63:2344–2354

    Article  CAS  PubMed  Google Scholar 

  41. Ngugi MP, Murugi JN, Kabiti CM, Ngeranwa JJ, Njue WM, Maina D, Gathumbi KP, Njagi NE (2011) Hypoglycemic activity of some Kenyan plants traditionally used to manage diabetes mellitus in Eastern Province. J Diabetes Metab 2(8)

  42. Rahmatollah R, Zahra H, Lootfollah F, Somayeh N, Sedigheh A, Zahra T (2014) Chromium level in prediction of diabetes in pre-diabetic patients. Adv Biomed Res 3:235

    Article  Google Scholar 

  43. Srivastava AK (2000) Anti-diabetic and toxic effects of vanadium compounds. Mol Cell Biochem 206(1–2):177–182

    Article  CAS  PubMed  Google Scholar 

  44. Ndip RN, Tanih NF, Kuete V (2010) Dialysis treatment is an independent risk factor for foot ulceration in patients with diabetes and stage 4 or 5 chronic kidney disease. Diabetes Care 33:1811–1816

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Gupta D, Raju J, Baquer NZ (1999) Modulation of some gluconeogenic enzymes in diabetic rat liver and kidney: effect of anti diabetic compounds. Indian J Exp Biol 37:196–199

    CAS  PubMed  Google Scholar 

  46. Gätjens J, Meier B, Adachi Y, Sakurai H, Rehder D (2008) Is vanadium a more versatile target in the activity of primordial life forms than hitherto anticipated? Biol Mol Chem 6:957–964

    Google Scholar 

Download references

Acknowledgements

The authors acknowledge the assistance given by the herbalists in the study sites for supplying medicinal plants used and the lab technicians in Chemistry department of Nairobi University in carrying out the research.

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Authors and Affiliations

  1. Chemistry Department, Kisii University, P.O Box 408-40200, Kisii, Kenya

    R. Mogwasi

  2. Forschungszentrum Juelich, Central Institute for Engineering, Electronics and Analytics, ZEA-3, Juelich, Germany

    S. Zor & V. Nischwitz

  3. Chemistry Department, University of Nairobi, P. O. Box 30197-00100, Nairobi, Kenya

    D. K. Kariuki

  4. Chemistry Department, Chuka University, P.O Box 109-60400, Chuka, Kenya

    M. Z. Getenga

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  1. R. Mogwasi
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  2. S. Zor
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  3. D. K. Kariuki
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  4. M. Z. Getenga
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  5. V. Nischwitz
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Correspondence to R. Mogwasi.

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The funding assistance for the work was given by Nacosti-Kenya.

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Mogwasi, R., Zor, S., Kariuki, D.K. et al. Sequential Extraction as Novel Approach to Compare 12 Medicinal Plants From Kenya Regarding Their Potential to Release Chromium, Manganese, Copper, and Zinc. Biol Trace Elem Res 182, 407–422 (2018). https://doi.org/10.1007/s12011-017-1083-2

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  • DOI: https://doi.org/10.1007/s12011-017-1083-2

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