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Selenium Ameliorates AFB1−Induced Excess Apoptosis in Chicken Splenocytes Through Death Receptor and Endoplasmic Reticulum Pathways

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Abstract

Aflatoxin B1 (AFB1) can cause hepatotoxicity, genotoxicity, and immunosuppressive effects for a variety of organisms. Selenium (Se), as an essential nutrient element, plays important protective effects against cell apoptosis induced by AFB1. This research aimed to reveal the ameliorative effects of selenium on AFB1-induced excess apoptosis in chicken splenocytes through death receptor and endoplasmic reticulum pathways in vivo. Two hundred sixteen neonatal chickens, randomized into four treatments, were fed with basal diet (control treatment), 0.4 mg/kg Se supplement (+Se treatment), 0.6 mg/kg AFB1 (AFB1 treatment), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se (AFB1 + Se treatment) during 21 days of experiment, respectively. Compared with the AFB1 treatment, the levels of splenocyte apoptosis in the AFB1 + Se treatment were obviously dropped by flow cytometry and TUNEL assays although they were still significantly higher than those in the control or + Se treatments. Furthermore, the mRNA expressions of CASP-3, CASP-8 and CASP-10, GRP78, GRP94, TNF-α, TNF-R1, FAS, and FASL of splenocytes in the AFB1 + Se treatment by qRT-PCR assay were significantly decreased compared with the AFB1 treatment. These results indicate that Se could partially ameliorate the AFB1-caused excessive apoptosis of chicken splenocytes through downregulation of endoplasmic reticulum and death receptor pathway molecules. This research may rich the knowledge of the detoxification mechanism of Se on AFB1-induced apoptosis.

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Funding

This work was supported by the program for Changjiang scholars, the University Innovative Research Team (IRT 0848), the Education Department of Sichuan Province (2012FZ0066) and (2013FZ0072) and Huimin project of Chengdu science and technology (2016-HM01-00337-SF).

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Author notes

  1. Jing Fang and Panpan Zhu contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Jing Fang, Panpan Zhu, Zhicai Zuo, Hengmin Cui, Ping Ouyang, Gang Shu, Zhengli Chen, Chao Huang & Wentao Liu

  2. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Jing Fang, Panpan Zhu, Zhicai Zuo & Hengmin Cui

  3. Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, 611130, Sichuan, People’s Republic of China

    Zhuangzhi Yang

  4. College of Life Sciences, China West Normal University, Nanchong, 637002, Sichuan, People’s Republic of China

    Xi Peng

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  1. Jing Fang
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Correspondence to Xi Peng or Zhicai Zuo.

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Fang, J., Zhu, P., Yang, Z. et al. Selenium Ameliorates AFB1−Induced Excess Apoptosis in Chicken Splenocytes Through Death Receptor and Endoplasmic Reticulum Pathways. Biol Trace Elem Res 187, 273–280 (2019). https://doi.org/10.1007/s12011-018-1361-7

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