Abstract
The marine bacteria Saccharophagus degradans (also known as Microbulbifer degradans), are rod-shaped and gram-negative motile γ-proteobacteria, capable of both degrading a variety of complex polysaccharides and fermenting monosaccharides into ethanol. In order to obtain insights into structure–function relationships of the enzymes, involved in these biochemical processes, we characterized a S. degradans β-glycosidase from glycoside hydrolase family 1 (SdBgl1B). SdBgl1B has the optimum pH of 6.0 and a melting temperature T m of approximately 50 °C. The enzyme has high specificity toward short d-glucose saccharides with β-linkages with the following preferences β-1,3 > β-1,4 ≫ β-1,6. The enzyme kinetic parameters, obtained using artificial substrates p-β-NPGlu and p-β-NPFuc and also the disaccharides cellobiose, gentiobiose and laminaribiose, revealed SdBgl1B preference for p-β-NPGlu and laminaribiose, which indicates its affinity for glucose and also preference for β-1,3 linkages. To better understand structural basis of the enzyme activity its 3D model was built and analysed. The 3D model fits well into the experimentally retrieved low-resolution SAXS-based envelope of the enzyme, confirming monomeric state of SdBgl1B in solution.
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Acknowledgments
The work was supported by the Grants from Brazilian funding agencies Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)#2008/56255-9; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)#490022/2009-0,#158752/2015-5 and by a collaborative Grant agreement between the University of Hamburg, Germany and the University of Sao Paulo (USP), Brazil.
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Brognaro, H., Almeida, V.M., de Araujo, E.A. et al. Biochemical Characterization and Low-Resolution SAXS Molecular Envelope of GH1 β-Glycosidase from Saccharophagus degradans . Mol Biotechnol 58, 777–788 (2016). https://doi.org/10.1007/s12033-016-9977-3
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DOI: https://doi.org/10.1007/s12033-016-9977-3