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An Antagomir to MicroRNA-106b-5p Ameliorates Cerebral Ischemia and Reperfusion Injury in Rats Via Inhibiting Apoptosis and Oxidative Stress

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Abstract

We previously observed that microRNA miR-106b-5p significantly increased in serum of patients with acute ischemic stroke. The present study was to determine whether miR-106b-5p antagomir can protect against cerebral ischemia/reperfusion (I/R) injury and elucidate its underlying mechanisms. Middle cerebral artery occlusion (MCAO) was operated on male Sprague Dawley rats. MiR-106b-5p antagomir significantly decreased neurological deficit scores, infarct volumes, and neuronal injury. Furthermore, miR-106b-5p antagomir markedly reduced malondialdehyde (MDA) content, restored superoxide dismutase (SOD) activity, increased the expression of myeloid cell leukemia-1 (Mcl-1) and B cell lymphoma-2 (Bcl-2), and decreased the expression of Bax in the ischemic cortex. In PC12 cells, miR-106b-5p inhibitor increased the Mcl-1 and Bcl-2 expression, which provided protection against glutamate-induced apoptosis and oxidative damage, as evidenced by decreased lactate dehydrogenase (LDH) release, and enhanced SOD activity. Notably, luciferase reported assay proved Mcl-1 was the target gene of miR-106b-5p. In conclusion, our data indicates that the neuroprotective effects of miR-106b-5p antagomir on cerebral I/R injury are associated with its inhibition of apoptosis and oxidative stress, suggesting a potential therapeutic target for ischemic stroke.

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Abbreviations

MiRNA:

MicroRNA

MCAO:

Middle cerebral artery occlusion

CNS:

Central nerve system

IS:

Ischemic stroke

I/R:

Ischemia/reperfusion

MDA:

Malondialdehyde

Bcl-2:

B cell lymphoma-2

LDH:

Lactate dehydrogenase

SOD:

Superoxide dismutase

ROS:

Reactive oxygen species

TTC:

2,3,5-Triphenyltetrazolium chloride

HE:

Hematoxylin and eosin

MRI:

Magnetic resonance imaging

FITC:

Fluorescein isothiocyanate

ROS:

Reactive oxygen species

3′-UTR:

3′-Untranslated region

MCM7:

Mini chromosome maintenance protein 7

SEM:

Standard error of the mean

PI:

Propidium iodide

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 81171659, 11574156, 81373748, and 81403136) and the 333 Project of Jiangsu Province in China (Grant No. BRA2014341). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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    Authors and Affiliations

    1. Department of Clinical Laboratory, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, Jiangsu, China

      Pengfei Li, Feng Gao, Fengmeng Teng & Chunbing Zhang

    2. The Second Clinical College, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China

      Meihong Shen

    3. Basic Medical Sciences, Nanjing University of Chinese Medicine, Nanjing, 210046, Jiangsu, China

      Jinping Wu, Jiahui Zhang & Chunbing Zhang

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    Correspondence to Chunbing Zhang.

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    This study was approved by the Animal Care and Use Committee of the Affiliated Hospital of Nanjing University of Chinese Medicine. All procedures were performed in accordance with the pertinent guidelines. All efforts were made to minimize animal suffering and the number of animals employed.

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    Pengfei Li and Meihong Shen contributed equally to this work.

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    Li, P., Shen, M., Gao, F. et al. An Antagomir to MicroRNA-106b-5p Ameliorates Cerebral Ischemia and Reperfusion Injury in Rats Via Inhibiting Apoptosis and Oxidative Stress. Mol Neurobiol 54, 2901–2921 (2017). https://doi.org/10.1007/s12035-016-9842-1

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