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Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation

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Abstract

Riboflavin transporters (rft-1 and rft-2), orthologous to human riboflavin transporter-3 (hRVFT-3), are identified and characterized in Caenorhabditis elegans. However, studies pertaining to functional contribution of rft-2 in maintaining body homeostatic riboflavin levels and its regulation are very limited. In this study, the expression pattern of rft-2 at different life stages of C. elegans was studied through real-time PCR, and found to be consistent from larval to adult stages that demonstrate its involvement in maintaining the body homeostatic riboflavin levels at whole animal level all through its life. A possible regulation of rft-2 expression at mRNA levels at whole animal was studied after adaptation to low and high concentrations of riboflavin. Abundance of rft-2 transcript was upregulated in riboflavin-deficient conditions (10 nM), while it was downregulated with riboflavin-supplemented conditions (2 mM) as compared with control (10 μM). Further, the 5′-regulatory region of the rft-2 gene was cloned, and transgenic nematodes expressing transcriptional rft-2 promoter::GFP fusion constructs were generated. The expression of rft-2 was found to be adaptively regulated in vivo when transgenic worms were maintained under different extracellular riboflavin levels, which was also mediated partly via changes in the rft-2 levels that directs towards the possible involvement of transcriptional regulatory events.

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Acknowledgments

We gratefully acknowledge University Grants Commission (UGC), India, for their financial support [F.NO/ 39-289/2010 (SR)] to BA. We thank Dr Shin Takagi, Nagoya University, Japan, for providing pPD95.75 vector as a generous gift; Dr K Balamurugan, Alagappa University, Karaikudi, for providing C. elegans Bristol N2 strain and E. coli OP50; UGC-NRCBS Centre, Madurai Kamaraj University, for extending imaging facilities; E. coli Genetic Stock Center, Yale University, USA, for riboflavin-deficient E. coli BSV13.

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Authors and Affiliations

  1. Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India

    Krishnan Gandhimathi, Sellamuthu Karthi, Paramasivam Manimaran & Balasubramaniem Ashokkumar

  2. Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625 021, India

    Perumal Varalakshmi

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  1. Krishnan Gandhimathi
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  2. Sellamuthu Karthi
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  3. Paramasivam Manimaran
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  4. Perumal Varalakshmi
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  5. Balasubramaniem Ashokkumar
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Correspondence to Balasubramaniem Ashokkumar.

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Corresponding editor: Seyed E Hasnain

[Gandhimathi K, Karthi S, Manimaran P, Varalakshmi P and Ashokkumar B 2015 Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation. J. Biosci. 40 1–12] DOI 10.1007/s12038-015-9512-x

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/jun2015/supp/Gandhimathi.pdf

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Gandhimathi, K., Karthi, S., Manimaran, P. et al. Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation. J Biosci 40, 257–268 (2015). https://doi.org/10.1007/s12038-015-9512-x

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  • DOI: https://doi.org/10.1007/s12038-015-9512-x

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