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Population structure and diversity assessment of barley (Hordeum vulgare L.) introduction from ICARDA

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Abstract

This study was undertaken to measure the genetic diversity and population structure of 48 barley accessions introduced from ICARDA using 51 polymorphic simple sequence repeat (SSR) markers to select unique parents for breeding. The mean polymorphic information content was 0.491, suggesting high polymorphism for the selected SSR markers among the barley accessions. The population structure indicated a fine genetic base only with two major clusters. All accessions had 100% membership probability in their respective clusters. Analysis of molecular variance revealed that most (78%) of the variation was attributed between populations, while 22% was due to variation among individuals within populations. Neighbour-joining (NJ) tree was constructed using this distance matrix and two major clusters were observed in it. Cluster 1 had all hulled barley accessions and cluster 2 had all hulless barley accessions. Cluster 2 could be further divided into three subclusters. Principal coordinates analysis results were similar to the NJ tree, where the hulled and hulless barley accessions were grouped into separate clusters. This study established the existence of considerable genetic diversity among the 48 tested accessions. The selected genetic resources will be useful for barley breeding in India and other countries.

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Acknowledgement

The authors gratefully acknowledge Mr Deepender Kumar (Ph.D. Scholar, ICAR-IIWBR, Karnal) for his assistance in statistical analysis.

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Authors and Affiliations

  1. Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, 474 002, India

    Pawan Kumar, Prabha Banjarey & A. N. Tikle

  2. ICAR-Indian Institute of Wheat and Barley Research, Karnal, 132 001, India

    Rekha Malik & R. P. S. Verma

  3. International Centre for Agricultural Research in the Dry Areas (ICARDA), 10000, Rabat, Morocco

    R. P. S. Verma

Authors
  1. Pawan Kumar
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  2. Prabha Banjarey
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  3. Rekha Malik
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  4. A. N. Tikle
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  5. R. P. S. Verma
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Correspondence to Pawan Kumar.

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Corresponding editor: Manoj Prasad

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Kumar, P., Banjarey, P., Malik, R. et al. Population structure and diversity assessment of barley (Hordeum vulgare L.) introduction from ICARDA. J Genet 99, 70 (2020). https://doi.org/10.1007/s12041-020-01226-6

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  • DOI: https://doi.org/10.1007/s12041-020-01226-6

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