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Strain Improvement of Streptomyces xanthochromogenes RIA 1098 for Enhanced Pravastatin Production at High Compactin Concentrations

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Abstract

Pravastatin is one of the most popular cholesterol-lowering drugs. Its industrial production represents a two-stage process including the microbial production of compactin and its further biocatalytic conversion to pravastatin. To increase a conversion rate, a higher compactin content in fermentation medium should be used; however, high compactin concentrations inhibit microbial growth. Therefore, the improvement of the compactin resistance of a producer still remains a relevant problem. A multi-step random UV mutagenesis of a Streptomyces xanthochromogenes strain RIA 1098 and the further selection of high-yield compactin-resistant mutants have resulted in a highly productive compactin-resistant strain S 33-1. After the fermentation medium improvement, the maximum bioconversion rate of this strain has reached 91 % at the daily compactin dose equal to 1 g/L and still remained high (83 %) even at the doubled dose (2 g/L). A 1-year study of the mutant strain stability has proved a stable inheritance of its characteristics that provides this strain to be very promising for the pravastatin-producing industry.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312, Russia

    Vakhtang V. Dzhavakhiya, Tatiana M. Voinova, Elena V. Glagoleva, Dmitry V. Petukhov, Alexander I. Ovchinnikov, Maksim I. Kartashov, Boris B. Kuznetsov & Konstantin G. Skryabin

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  1. Vakhtang V. Dzhavakhiya
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  2. Tatiana M. Voinova
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  3. Elena V. Glagoleva
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  4. Dmitry V. Petukhov
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  5. Alexander I. Ovchinnikov
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  6. Maksim I. Kartashov
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  7. Boris B. Kuznetsov
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  8. Konstantin G. Skryabin
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Correspondence to Vakhtang V. Dzhavakhiya.

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Dzhavakhiya, V.V., Voinova, T.M., Glagoleva, E.V. et al. Strain Improvement of Streptomyces xanthochromogenes RIA 1098 for Enhanced Pravastatin Production at High Compactin Concentrations. Indian J Microbiol 55, 440–446 (2015). https://doi.org/10.1007/s12088-015-0537-5

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  • DOI: https://doi.org/10.1007/s12088-015-0537-5

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