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Prediction of Transcription Factors and Their Involvement in Regulating Rifamycin Production in Amycolatopsis mediterranei S699

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Abstract

Amycolatopsis mediterranei S699 produces rifamycin B and successors of this strain are in use for the industrial production of rifamycin B. Semisynthetic derivatives of rifamycin B are used against Mycobacterium tuberculosis that causes tuberculosis. Although the rifamycin biosynthetic gene cluster was characterized two decades ago, the regulation of rifamycin B biosynthesis in Amycolatopsis mediterranei S699 is poorly understood. In this study, we analysed the genome and proteome of Amycolatopsis mediterranei S699 and identified 1102 transcription factors which comprise about 10% of the total genome. Using interactomics approaches we delineated 30 unique transcription factors directly involved in secondary metabolism that regulate rifamycin B biosynthesis. We also predict the role of RifN as hub in controlling the regulation of other genes involved in rifamycin biosynthesis. RifN is important for maintaining the integrity of the rifamycin-network. Thus, these transcription factor can be exploited to improve rifamycin B production in Amycolatopsis mediterranei S699.

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Acknowledgements

RL acknowledges The National Academy of Sciences, India, for support under the NASI‐Senior Scientist Platinum Jubilee Fellowship Scheme. NS acknowledges Council of Scientific and Industrial Research, New Delhi (CSIR) for doctoral fellowship.

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Author notes

  1. Priya Singh

    Present address: Acharya Narendra Dev College, University of Delhi, New Delhi, 110019, India

  2. Nirjara Singhvi and Vipin Gupta have contributed equally.

Authors and Affiliations

  1. Department of Zoology, University of Delhi, Delhi, 110007, India

    Nirjara Singhvi, Vipin Gupta, Priya Singh & Yogendra Singh

  2. National Centre for Cell Sciences, Pune, Maharashtra, 411007, India

    Om Prakash

  3. Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs University, 79104, Freiburg, Germany

    Andreas Bechthold

  4. The Energy and Resources Institute, Darbari Seth Block, IHC Complex, New Delhi, 110033, India

    Rup Lal

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  1. Nirjara Singhvi
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Singhvi, N., Gupta, V., Singh, P. et al. Prediction of Transcription Factors and Their Involvement in Regulating Rifamycin Production in Amycolatopsis mediterranei S699. Indian J Microbiol 60, 310–317 (2020). https://doi.org/10.1007/s12088-020-00868-5

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