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Sequence-specific resonance assignments of human TAF15-RRM and TAF15-RRM-RanBP2

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Abstract

Human TATA binding protein associated factor 2 N (TAF15) is a RNA/DNA binding protein involved in many aspects of RNA and DNA metabolism. TAF15 contains an N-terminal transcriptional activation domain and C-terminal region comprising the RNA recognition motif (RRM) and RanBP2 type zinc finger domains with interspersed RGG motifs. In this study we report the complete backbone and side chain resonance assignments of human TAF15-RRM and backbone assignments of TAF15-RRM-RanBP2.

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References

  • Baechtold H, Kuroda M, Sok J, Ron D, Lopez BS, Akhmedov AT (1999) Human 75-kDa DNA-pairing protein is identical to the pro-oncoprotein TLS/FUS and is able to promote D-loop formation. J Biol Chem 274:34337–34342

    Article  Google Scholar 

  • Bertolotti A, Bell B, Tora L (1999) The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties. Oncogene 18:8000–8010

    Article  Google Scholar 

  • Hoell JI, Larsson E, Runge S, Nusbaum JD, Duggimpudi S, Farazi TA et al (2011) RNA targets of wild-type and mutant FET family proteins. Nat Struct Mol Biol 18:1428–1431

    Article  Google Scholar 

  • Ibrahim F, Maragkakis M, Alexiou P, Maronski MA, Dichter MA, Mourelatos Z (2013) Identification of in vivo, conserved, TAF15 RNA binding sites reveals the impact of TAF15 on the neuronal transcriptome. Cell Rep 3:301–308

    Article  Google Scholar 

  • Iko Y, Kodama TS, Kasai N, Oyama T, Morita EH, Muto T et al (2004) Domain architectures and characterization of an RNA-binding protein, TLS. J Biol Chem 279:44834–44840

    Article  Google Scholar 

  • Keller R (2004) The Computer Aided Resonance Assignment Tutorial. 1st edn. ISBN 3-85600- 112-3, CANTINA Verlag

  • Liu X, Niu C, Ren J, Zhang J, Xie X, Zhu H et al (2013) The RRM domain of human fused in sarcoma protein reveals a non-canonical nucleic acid binding site. Biochim Biophys Acta 1832:375–385

    Article  Google Scholar 

  • Nguyen CD, Mansfield RE, Leung W, Vaz PM, Loughlin FE, Grant RP et al (2011) Characterization of a family of RanBP2-type zinc fingers that can recognize single-stranded RNA. J Mol Biol 407:273–283

    Article  Google Scholar 

  • Tan AY, Manley JL (2009) The TET family of proteins: functions and roles in disease. J Mol Cell Biol 1:82–92

    Article  Google Scholar 

  • Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4:171–180

    Article  Google Scholar 

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Acknowledgments

We thank Department of Biotechnology (DBT), Government of India for providing financial support for the High Field NMR spectrometers at the ICGEB, New Delhi and NII, New Delhi. MK is a recipient of Indian Council for medical Research (ICMR) senior research fellowship. AKG is a recipient of Department of Biotechnology senior research fellowship. This study is supported by grant to NSB from the Department of Science and Technology (DST), Government of India and  ICGEB core funds.

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  1. Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India

    Maruthi Kashyap, Akshay Kumar Ganguly & Neel Sarovar Bhavesh

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  1. Maruthi Kashyap
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  2. Akshay Kumar Ganguly
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  3. Neel Sarovar Bhavesh
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Correspondence to Neel Sarovar Bhavesh.

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Kashyap, M., Ganguly, A.K. & Bhavesh, N.S. Sequence-specific resonance assignments of human TAF15-RRM and TAF15-RRM-RanBP2. Biomol NMR Assign 9, 103–106 (2015). https://doi.org/10.1007/s12104-014-9553-3

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  • DOI: https://doi.org/10.1007/s12104-014-9553-3

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