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Enhanced Performance of Rhizopus oryzae Lipase Immobilized on Hydrophobic Carriers and Its Application in Biorefinery of Rapeseed Oil Deodorizer Distillate

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Abstract

In this study, hydrophobic macroporous resin NKA was employed as matrix for immobilization of free Rhizopus oryzae lipase (ROL). The performance of the immobilized ROL was significantly enhanced. The recovery activity was up to 1,293.78 % and the specific activity increased to 152,914 U/g-protein, which was 46-fold higher than that of the free lipase. Moreover, the immobilized lipase showed higher thermostability and better pH-resistance than its free counterpart. Additionally, three different nonaqueous modification strategies (including bioimprinting, lecithin coating, and lyophilization protection) were further utilized to improve the performance of the immobilized lipase. The corresponding enhancements were 33.68 %, 31.98 %, and 99.86 %. When these modifications were combined together, the activity improved 209.51 %. In order to confirm its practical application, the modified ROL was used to biorefine rapeseed oil deodorizer distillate (RODD) for biodiesel production. The highest conversion yield reached 98.23 %, much close to that (97.46 %) of Novozym 435. The results suggest that the prepared lipase in this study is a promising biocatalyst with high stability, efficiency and operational reusability.

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Abbreviations

DD:

Deodorizer distillate

RODD:

Rapeseed oil deodorizer distillate

FFA:

Free fatty acid

ROL:

Rhizopus oryzae lipase

Mtpa:

Million tons per annum

BSA:

Bovine serum albumin

NOV 435:

Novozym 435

FAEEs:

Fatty acid ethyl esters

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Acknowledgments

The authors acknowledge the financial support of the National Natural Science Foundation of P. R. China (NSFC; Nos. 31070089, 31170078, and J1103514), the National High Technology Research and Development Program of P. R. China (863 Program; Nos. 2011AA02A204, 2013AA065805), and the Innovation Foundation of Shenzhen Government (JCYJ20120831111657864). Many thanks are indebted to Ms Chen Hong, from the Centre of Analysis and Test, Huazhong University of Science and Technology for biodiesel analysis.

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Authors and Affiliations

  1. Key Laboratory of Molecular Biophysics, The Ministry of Education; College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Feng Su, Guanlin Li, Houjin Zhang & Yunjun Yan

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  1. Feng Su
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Correspondence to Yunjun Yan.

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Su, F., Li, G., Zhang, H. et al. Enhanced Performance of Rhizopus oryzae Lipase Immobilized on Hydrophobic Carriers and Its Application in Biorefinery of Rapeseed Oil Deodorizer Distillate. Bioenerg. Res. 7, 935–945 (2014). https://doi.org/10.1007/s12155-014-9415-y

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