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Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord

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Abstract

We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton’s jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1–2 mm³ fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.

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Authors and Affiliations

  1. Department of Cell Processing and Transfusion, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan

    Ikuo Ishige, Tokiko Nagamura-Inoue & Arinobu Tojo

  2. Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan

    Ikuo Ishige & Hiromitsu Nakauchi

  3. Department of Anatomy, School of Dentistry, Nihon University, Kanda-Surugadai 1-8-13, Chiyoda-ku, Tokyo, 101-8310, Japan

    Masaki J. Honda

  4. Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639, Japan

    Ratanakanit Harnprasopwat & Arinobu Tojo

  5. Department of Obstetrics, Japanese Red Cross Medical Center, Hiroo 4-1-22, Shibuya-ku, Tokyo, 150-8935, Japan

    Michiko Kido & Mitsuhiro Sugimoto

Authors
  1. Ikuo Ishige
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  2. Tokiko Nagamura-Inoue
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  3. Masaki J. Honda
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  4. Ratanakanit Harnprasopwat
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  5. Michiko Kido
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  6. Mitsuhiro Sugimoto
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  7. Hiromitsu Nakauchi
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  8. Arinobu Tojo
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Correspondence to Tokiko Nagamura-Inoue.

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Ishige, I., Nagamura-Inoue, T., Honda, M.J. et al. Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord. Int J Hematol 90, 261–269 (2009). https://doi.org/10.1007/s12185-009-0377-3

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  • DOI: https://doi.org/10.1007/s12185-009-0377-3

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