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Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection

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Abstract

Drugs that lack the ability to cross the blood-brain barrier (BBB) need to be placed directly into the central nervous system. Our laboratory studies the involvement of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebellomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solution. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.

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Acknowledgements

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – Edital Doenças Neurodegenerativas, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), and Financiadora de Estados e Projetos (FINEP).

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  1. Thainá Garbino dos Santos and Mery Stéfani Leivas Pereira have contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Cellular Neurochemistry, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil

    Thainá Garbino dos Santos, Mery Stéfani Leivas Pereira & Diogo Losch Oliveira

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  1. Thainá Garbino dos Santos
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  2. Mery Stéfani Leivas Pereira
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  3. Diogo Losch Oliveira
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Correspondence to Thainá Garbino dos Santos.

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dos Santos, T.G., Pereira, M.S.L. & Oliveira, D.L. Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection. Neurosci. Bull. 34, 827–832 (2018). https://doi.org/10.1007/s12264-018-0223-9

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  • DOI: https://doi.org/10.1007/s12264-018-0223-9

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