Abstract
We demonstrated the effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. To investigate the effect of exosomes from C-MSC on angiogenesis, we injected C-MSC-Exo or PBS intramuscularly into ischemic hind limb. Blood perfusion of limb was evaluated by laser Doppler Imaging. We observed that ischemic limb treated with C-MSC-Exo exhibits improved blood perfusion compared to ischemic limb treated with PBS at 2 weeks and 1 month after induction of limb ischemia. To explore the potential mechanisms underlying C-MSC-Exo’s angiogenetic effect, we performed microRNA array analysis and identify mmu-miR-7116-5p as the most abundant enriched miRNA detected in C-MSC-Exo. Bioinformatics’ analysis shows that miR-7116-5p negatively regulates protein polyubiquitination. In conclusion, our study demonstrated that intramuscular delivery of C-MSC-Exo after limb ischemia improves blood perfusion, and we identified the most abundant miRNAs that are preferentially enriched in C-MSC-Exo.
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Y. Tang was partially supported by the American Heart Association. GRNT31430008, NIH-AR070029, NIH-HL086555, NIH-HL134354.
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The authors declare that they have no conflict of interest.
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Animals were treated according to the approved protocols and animal welfare regulations of the Institutional Animal Care and Use Committee of the Medical College of Georgia, Augusta University. This article does not contain any studies with human participants performed by any of the authors.
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Associate Editor Enrique Lara-Pezzi oversaw the review of this article
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Ju, C., Li, Y., Shen, Y. et al. Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis. J. of Cardiovasc. Trans. Res. 11, 429–437 (2018). https://doi.org/10.1007/s12265-018-9824-y
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DOI: https://doi.org/10.1007/s12265-018-9824-y